Rapid method of extraction and analysis of extended-spectrum β-lactamases from clinical strains of Klebsiella pneumoniae
The extraction of periplasmic β-lactamases from Gram-negative bacilli is a necessary preliminary step to analytical isoelectric focusing. Previously described methods are time-consuming and require large amounts of broth. We describe a lysozyme-based method which needs just 5 mL broth and requires l...
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| Veröffentlicht in: | Clinical microbiology and infection 2001-12, Vol.7 (12), p.709-711 |
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| container_title | Clinical microbiology and infection |
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| creator | Paterson, D.L. Rice, L.B. Bonomo, R.A. |
| description | The extraction of periplasmic β-lactamases from Gram-negative bacilli is a necessary preliminary step to analytical isoelectric focusing. Previously described methods are time-consuming and require large amounts of broth. We describe a lysozyme-based method which needs just 5 mL broth and requires less than 24 h to perform. The method was reproducible in extracting β-lactamases from reference strains containing known β-lactamases. We applied the method to a collection of more than 70 extended-spectrum β-lactamase-producing isolates from a multinational study of bacteremic isolates of Klebsiella pneumoniae. Further studies are being undertaken to assess the method's applicability to other bacterial species. |
| doi_str_mv | 10.1046/j.1469-0691.2001.00363.x |
| format | Article |
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Previously described methods are time-consuming and require large amounts of broth. We describe a lysozyme-based method which needs just 5 mL broth and requires less than 24 h to perform. The method was reproducible in extracting β-lactamases from reference strains containing known β-lactamases. We applied the method to a collection of more than 70 extended-spectrum β-lactamase-producing isolates from a multinational study of bacteremic isolates of Klebsiella pneumoniae. 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| ispartof | Clinical microbiology and infection, 2001-12, Vol.7 (12), p.709-711 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | beta-Lactamases - analysis beta-Lactamases - isolation & purification Humans Isoelectric Focusing Klebsiella pneumoniae - enzymology Muramidase |
| title | Rapid method of extraction and analysis of extended-spectrum β-lactamases from clinical strains of Klebsiella pneumoniae |
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