Detection of hairy cell leukaemia in blood and bone marrow using multidimensional flow cytometry with CD45-PECy5 and SS gating

CD45 and right angle light scatter (SS) gating are used commonly in clinical flow cytometry to differentiate cells of various lineages (Stelzer et al., 1993). We have used CD45‐PECy5 (Clone J33) since 1998 and have noticed that malignant lymphoid cells such as hairy cells can form distinct populatio...

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Veröffentlicht in:	Clinical and laboratory haematology 2001-12, Vol.23 (6), p.385-390
Hauptverfasser:	Tytherleigh, L., Taparia, M., Leahy, M.F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Blood Cells - pathology Bone Marrow Cells - pathology CD45-PECy5 flow cytometry Flow Cytometry - methods Hairy cell leukaemia Humans Immunophenotyping - instrumentation Immunophenotyping - methods Leukemia, Hairy Cell - diagnosis Leukemia, Hairy Cell - immunology Leukemia, Hairy Cell - pathology Leukocyte Common Antigens - analysis Leukocyte Common Antigens - immunology mean cell fluorescence Scattering, Radiation
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