A Fluorescence-Based Method for Determining the Surface Coverage and Hybridization Efficiency of Thiol-Capped Oligonucleotides Bound to Gold Thin Films and Nanoparticles

Using a fluorescence-based method, we have determined the number of thiol-derivatized single-stranded oligonucleotides bound to gold nanoparticles and their extent of hybridization with complementary oligonucleotides in solution. Oligonucleotide surface coverages of hexanethiol 12-mer oligonucleotid...

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Veröffentlicht in:Analytical chemistry (Washington) 2000-11, Vol.72 (22), p.5535-5541
Hauptverfasser: Demers, Linette M, Mirkin, Chad A, Mucic, Robert C, Reynolds, Robert A, Letsinger, Robert L, Elghanian, Robert, Viswanadham, Garimella
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Sprache:eng
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