A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs
The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at...
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| Veröffentlicht in: | Life sciences (1973) 2000-10, Vol.67 (23), p.2889-2896 |
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| container_end_page | 2896 |
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| container_issue | 23 |
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| container_title | Life sciences (1973) |
| container_volume | 67 |
| creator | Lam, T.L. Lam, M.L. Au, T.K. Ip, D.T.M. Ng, T.B. Fong, W.P. Wan, D.C.C. |
| description | The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their
in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of
Prunella vulgaris and
Scutellaria baicalensis and the methanol extracts of
Woodwardia unigemmata,
Paeonica suffruticosa and
Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 μg/ml. |
| doi_str_mv | 10.1016/S0024-3205(00)00864-X |
| format | Article |
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in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of
Prunella vulgaris and
Scutellaria baicalensis and the methanol extracts of
Woodwardia unigemmata,
Paeonica suffruticosa and
Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 μg/ml.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/S0024-3205(00)00864-X</identifier><identifier>PMID: 11106004</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Chinese medicinal herbs ; Fluorogenic assay ; HIV Protease - metabolism ; HIV Protease Inhibitors - pharmacology ; HIV-1 - enzymology ; HIV-1 protease ; HPLC assay ; Human immunodeficiency virus 1 ; Humans ; Kinetics ; Magnoliopsida ; Medicine, Chinese Traditional ; Methanol ; Phytotherapy ; Plant Extracts - pharmacology ; Prunella vulgaris ; Recombinant Proteins - antagonists & inhibitors ; Water</subject><ispartof>Life sciences (1973), 2000-10, Vol.67 (23), p.2889-2896</ispartof><rights>2000 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-841e79f5b970c81337d0f9d287613ef6f6e68f7b21301a26e942a9a97e825edb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0024-3205(00)00864-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11106004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lam, T.L.</creatorcontrib><creatorcontrib>Lam, M.L.</creatorcontrib><creatorcontrib>Au, T.K.</creatorcontrib><creatorcontrib>Ip, D.T.M.</creatorcontrib><creatorcontrib>Ng, T.B.</creatorcontrib><creatorcontrib>Fong, W.P.</creatorcontrib><creatorcontrib>Wan, D.C.C.</creatorcontrib><title>A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their
in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of
Prunella vulgaris and
Scutellaria baicalensis and the methanol extracts of
Woodwardia unigemmata,
Paeonica suffruticosa and
Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 μg/ml.</description><subject>Chinese medicinal herbs</subject><subject>Fluorogenic assay</subject><subject>HIV Protease - metabolism</subject><subject>HIV Protease Inhibitors - pharmacology</subject><subject>HIV-1 - enzymology</subject><subject>HIV-1 protease</subject><subject>HPLC assay</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Magnoliopsida</subject><subject>Medicine, Chinese Traditional</subject><subject>Methanol</subject><subject>Phytotherapy</subject><subject>Plant Extracts - pharmacology</subject><subject>Prunella vulgaris</subject><subject>Recombinant Proteins - antagonists & inhibitors</subject><subject>Water</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EYpqBTwB5hWARqMrDjldo1OIljcQCkGZnOUlFMUrsxnZa07_BF-N-CJazshfn1q26l7GXCO8QULz_DlDWRVVC8wbgLUAr6uLuEdtgK1UBosLHbPMPuWLPYvwFAE0jq6fsChFBANQb9ueG937ZmWCjd9yPfFoX47hdltX5gUbbW3L9ge9tWCNPhx0VyHfBJzKRuHWT7WyyWWr6ZPf5S5F3B54m4ub3Sj6LjBv4Qmkyzs-c7lPIaDxabSfrKE9ZaMg2zsx8otDF5-zJaOZILy7vNfv56eOP7Zfi9tvnr9ub26JvEFLR1khSjU2nJPQtVpUcYFRD2UqBFY1iFCTaUXYlVoCmFKTq0iijJLVlQ0NXXbPX57n5nLxqTHqxsad5Nu64t5ZlXUms4UEQpSyVqjGDzRnsg48x0Kh3wS4mHDSCPramT63pYyUaQJ9a03dZ9-pisHY5jP-qS00Z-HAGKOextxR0PPWSgwvUJz14-4DFX0apqXc</recordid><startdate>20001027</startdate><enddate>20001027</enddate><creator>Lam, T.L.</creator><creator>Lam, M.L.</creator><creator>Au, T.K.</creator><creator>Ip, D.T.M.</creator><creator>Ng, T.B.</creator><creator>Fong, W.P.</creator><creator>Wan, D.C.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20001027</creationdate><title>A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs</title><author>Lam, T.L. ; Lam, M.L. ; Au, T.K. ; Ip, D.T.M. ; Ng, T.B. ; Fong, W.P. ; Wan, D.C.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-841e79f5b970c81337d0f9d287613ef6f6e68f7b21301a26e942a9a97e825edb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Chinese medicinal herbs</topic><topic>Fluorogenic assay</topic><topic>HIV Protease - metabolism</topic><topic>HIV Protease Inhibitors - pharmacology</topic><topic>HIV-1 - enzymology</topic><topic>HIV-1 protease</topic><topic>HPLC assay</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Magnoliopsida</topic><topic>Medicine, Chinese Traditional</topic><topic>Methanol</topic><topic>Phytotherapy</topic><topic>Plant Extracts - pharmacology</topic><topic>Prunella vulgaris</topic><topic>Recombinant Proteins - antagonists & inhibitors</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lam, T.L.</creatorcontrib><creatorcontrib>Lam, M.L.</creatorcontrib><creatorcontrib>Au, T.K.</creatorcontrib><creatorcontrib>Ip, D.T.M.</creatorcontrib><creatorcontrib>Ng, T.B.</creatorcontrib><creatorcontrib>Fong, W.P.</creatorcontrib><creatorcontrib>Wan, D.C.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lam, T.L.</au><au>Lam, M.L.</au><au>Au, T.K.</au><au>Ip, D.T.M.</au><au>Ng, T.B.</au><au>Fong, W.P.</au><au>Wan, D.C.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2000-10-27</date><risdate>2000</risdate><volume>67</volume><issue>23</issue><spage>2889</spage><epage>2896</epage><pages>2889-2896</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their
in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of
Prunella vulgaris and
Scutellaria baicalensis and the methanol extracts of
Woodwardia unigemmata,
Paeonica suffruticosa and
Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 μg/ml.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>11106004</pmid><doi>10.1016/S0024-3205(00)00864-X</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Chinese medicinal herbs Fluorogenic assay HIV Protease - metabolism HIV Protease Inhibitors - pharmacology HIV-1 - enzymology HIV-1 protease HPLC assay Human immunodeficiency virus 1 Humans Kinetics Magnoliopsida Medicine, Chinese Traditional Methanol Phytotherapy Plant Extracts - pharmacology Prunella vulgaris Recombinant Proteins - antagonists & inhibitors Water |
| title | A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs |
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