Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii

Abstract A multiplex PCR assay with five primers targeting the 16S and 23S rRNA genes was developed for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. The selected primers amplify a 257-bp fragment from A. cryaerophilus, a 401...

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Veröffentlicht in:	FEMS microbiology letters 2000-12, Vol.193 (1), p.89-94
Hauptverfasser:	Houf, Kurt, Tutenel, Ann, De Zutter, Lieven, Van Hoof, Jan, Vandamme, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arcobacter Arcobacter - classification Arcobacter - genetics Arcobacter - isolation & purification Arcobacter butzleri Arcobacter cryaerophilus Assaying Bacteriology Biological and medical sciences Biotechnology Campylobacter Cefoperazone Chickens - microbiology Cultivation Culture Media DNA Primers DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Ribosomal - analysis DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Genes, rRNA Genetic engineering Genetic technics In vitro gene amplification. Pcr technique Meat - microbiology Methods. Procedures. Technologies Microbiology Multiplex PCR Multiplexing Polymerase Chain Reaction - methods Poultry RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics rRNA 16S rRNA 23S Sensitivity and Specificity Skin - microbiology Systematics Teicoplanin
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