Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii
Abstract A multiplex PCR assay with five primers targeting the 16S and 23S rRNA genes was developed for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. The selected primers amplify a 257-bp fragment from A. cryaerophilus, a 401...
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| Veröffentlicht in: | FEMS microbiology letters 2000-12, Vol.193 (1), p.89-94 |
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| creator | Houf, Kurt Tutenel, Ann De Zutter, Lieven Van Hoof, Jan Vandamme, Peter |
| description | Abstract
A multiplex PCR assay with five primers targeting the 16S and 23S rRNA genes was developed for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. The selected primers amplify a 257-bp fragment from A. cryaerophilus, a 401-bp fragment from A. butzleri and a 641-bp fragment from A. skirrowii. No PCR product was generated for closely related bacteria including Campylobacter and Helicobacter species. The assay was useful to identify cultures after in vitro cultivation and to detect and identify A. butlzeri and A. cryaerophilus from poultry samples present in 24-h old enrichment in Arcobacter broth with cefoperazone, amphotericin and teicoplanin (CAT)-supplement. |
| doi_str_mv | 10.1111/j.1574-6968.2000.tb09407.x |
| format | Article |
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A multiplex PCR assay with five primers targeting the 16S and 23S rRNA genes was developed for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. The selected primers amplify a 257-bp fragment from A. cryaerophilus, a 401-bp fragment from A. butzleri and a 641-bp fragment from A. skirrowii. No PCR product was generated for closely related bacteria including Campylobacter and Helicobacter species. The assay was useful to identify cultures after in vitro cultivation and to detect and identify A. butlzeri and A. cryaerophilus from poultry samples present in 24-h old enrichment in Arcobacter broth with cefoperazone, amphotericin and teicoplanin (CAT)-supplement.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2000.tb09407.x</identifier><identifier>PMID: 11094284</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Arcobacter ; Arcobacter - classification ; Arcobacter - genetics ; Arcobacter - isolation & purification ; Arcobacter butzleri ; Arcobacter cryaerophilus ; Assaying ; Bacteriology ; Biological and medical sciences ; Biotechnology ; Campylobacter ; Cefoperazone ; Chickens - microbiology ; Cultivation ; Culture Media ; DNA Primers ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; DNA, Ribosomal - analysis ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; Genetic engineering ; Genetic technics ; In vitro gene amplification. Pcr technique ; Meat - microbiology ; Methods. Procedures. Technologies ; Microbiology ; Multiplex PCR ; Multiplexing ; Polymerase Chain Reaction - methods ; Poultry ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 23S - genetics ; rRNA 16S ; rRNA 23S ; Sensitivity and Specificity ; Skin - microbiology ; Systematics ; Teicoplanin</subject><ispartof>FEMS microbiology letters, 2000-12, Vol.193 (1), p.89-94</ispartof><rights>2000 Federation of European Microbiological Societies 2000</rights><rights>2000 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4189-6d834a23a7ac689fb3ca7c63215e67d0ec329d93074517f30b87bc6aa4bdae163</citedby><cites>FETCH-LOGICAL-c4189-6d834a23a7ac689fb3ca7c63215e67d0ec329d93074517f30b87bc6aa4bdae163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2000.tb09407.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2000.tb09407.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5692016$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11094284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Houf, Kurt</creatorcontrib><creatorcontrib>Tutenel, Ann</creatorcontrib><creatorcontrib>De Zutter, Lieven</creatorcontrib><creatorcontrib>Van Hoof, Jan</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><title>Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
A multiplex PCR assay with five primers targeting the 16S and 23S rRNA genes was developed for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. The selected primers amplify a 257-bp fragment from A. cryaerophilus, a 401-bp fragment from A. butzleri and a 641-bp fragment from A. skirrowii. No PCR product was generated for closely related bacteria including Campylobacter and Helicobacter species. The assay was useful to identify cultures after in vitro cultivation and to detect and identify A. butlzeri and A. cryaerophilus from poultry samples present in 24-h old enrichment in Arcobacter broth with cefoperazone, amphotericin and teicoplanin (CAT)-supplement.</description><subject>Animals</subject><subject>Arcobacter</subject><subject>Arcobacter - classification</subject><subject>Arcobacter - genetics</subject><subject>Arcobacter - isolation & purification</subject><subject>Arcobacter butzleri</subject><subject>Arcobacter cryaerophilus</subject><subject>Assaying</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Campylobacter</subject><subject>Cefoperazone</subject><subject>Chickens - microbiology</subject><subject>Cultivation</subject><subject>Culture Media</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - analysis</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>In vitro gene amplification. Pcr technique</subject><subject>Meat - microbiology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Multiplex PCR</subject><subject>Multiplexing</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Poultry</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>rRNA 16S</subject><subject>rRNA 23S</subject><subject>Sensitivity and Specificity</subject><subject>Skin - microbiology</subject><subject>Systematics</subject><subject>Teicoplanin</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkd-K1DAUxoMo7uzqK0hQ2Stb869JK3ixjK4KI4rodUjTlM2YabpJ6874ND6q6basiyhibgLn_L4v5-QD4DFGOU7n-TbHhWAZr3iZE4RQPtSoYkjk-ztgddO6C1aIijLDqBJH4DjGbUIZQfw-OMKpyEjJVuDHK_PNON_vTDdA30IFd6MbbO_MHn5cf4IqRnWArQ9wuDAw2qmrOuPHCBszGD1Y30HVNdA2ycG2VqvrUrI6C9rXSg8mwHocvjsT7LPbRR0OygTfX1iX3CaPW8341Ybgr6x9AO61ykXzcLlPwJfz15_Xb7PNhzfv1mebTDNcVhlvSsoUoUoozcuqralWQnNKcGG4aJDRlFRNRZFgBRYtRXUpas2VYnWjDOb0BJzOvn3wl6OJg9zZqI1z87ZSEEYZ4dU_QSwEwwUqEvjkN3Drx9ClJSShKQEuuGCJejFTOvgYg2llH-xOhYPESE5xy62cMpVTpnKKWy5xy30SP1qeGOudaX5Jl3wT8HQBVNTKtUF12sYbruAVQdfLv5yxK-vM4T8mkOfvN-X0J8Ws92P_F3X2p_l_Auuc2cA</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Houf, Kurt</creator><creator>Tutenel, Ann</creator><creator>De Zutter, Lieven</creator><creator>Van Hoof, Jan</creator><creator>Vandamme, Peter</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200012</creationdate><title>Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii</title><author>Houf, Kurt ; Tutenel, Ann ; De Zutter, Lieven ; Van Hoof, Jan ; Vandamme, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4189-6d834a23a7ac689fb3ca7c63215e67d0ec329d93074517f30b87bc6aa4bdae163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Arcobacter</topic><topic>Arcobacter - classification</topic><topic>Arcobacter - genetics</topic><topic>Arcobacter - isolation & purification</topic><topic>Arcobacter butzleri</topic><topic>Arcobacter cryaerophilus</topic><topic>Assaying</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Campylobacter</topic><topic>Cefoperazone</topic><topic>Chickens - microbiology</topic><topic>Cultivation</topic><topic>Culture Media</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - analysis</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>In vitro gene amplification. Pcr technique</topic><topic>Meat - microbiology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Multiplex PCR</topic><topic>Multiplexing</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Poultry</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>rRNA 16S</topic><topic>rRNA 23S</topic><topic>Sensitivity and Specificity</topic><topic>Skin - microbiology</topic><topic>Systematics</topic><topic>Teicoplanin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Houf, Kurt</creatorcontrib><creatorcontrib>Tutenel, Ann</creatorcontrib><creatorcontrib>De Zutter, Lieven</creatorcontrib><creatorcontrib>Van Hoof, Jan</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Houf, Kurt</au><au>Tutenel, Ann</au><au>De Zutter, Lieven</au><au>Van Hoof, Jan</au><au>Vandamme, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2000-12</date><risdate>2000</risdate><volume>193</volume><issue>1</issue><spage>89</spage><epage>94</epage><pages>89-94</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
A multiplex PCR assay with five primers targeting the 16S and 23S rRNA genes was developed for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. The selected primers amplify a 257-bp fragment from A. cryaerophilus, a 401-bp fragment from A. butzleri and a 641-bp fragment from A. skirrowii. No PCR product was generated for closely related bacteria including Campylobacter and Helicobacter species. The assay was useful to identify cultures after in vitro cultivation and to detect and identify A. butlzeri and A. cryaerophilus from poultry samples present in 24-h old enrichment in Arcobacter broth with cefoperazone, amphotericin and teicoplanin (CAT)-supplement.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11094284</pmid><doi>10.1111/j.1574-6968.2000.tb09407.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2000-12, Vol.193 (1), p.89-94 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Arcobacter Arcobacter - classification Arcobacter - genetics Arcobacter - isolation & purification Arcobacter butzleri Arcobacter cryaerophilus Assaying Bacteriology Biological and medical sciences Biotechnology Campylobacter Cefoperazone Chickens - microbiology Cultivation Culture Media DNA Primers DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Ribosomal - analysis DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Genes, rRNA Genetic engineering Genetic technics In vitro gene amplification. Pcr technique Meat - microbiology Methods. Procedures. Technologies Microbiology Multiplex PCR Multiplexing Polymerase Chain Reaction - methods Poultry RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics rRNA 16S rRNA 23S Sensitivity and Specificity Skin - microbiology Systematics Teicoplanin |
| title | Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii |
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