Validity of vaginal testing in detecting human papillomavirus (HPV) genotypes
The aim of this study was to assess the validity and usefulness of vaginal scrapes in detecting cervical human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR). The study group comprised 23 women tested positive and 28 women tested negative for cervical HPV DNA by PCR, and confirmed b...
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| Veröffentlicht in: | Journal of clinical virology 2000-12, Vol.19 (3), p.163-168 |
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| creator | Finan, Ramzi R Irani-Hakime, Noha Tamim, Hala Almawi, Wassim Y |
| description | The aim of this study was to assess the validity and usefulness of vaginal scrapes in detecting cervical human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR). The study group comprised 23 women tested positive and 28 women tested negative for cervical HPV DNA by PCR, and confirmed by histopathology. At the time of specimen collection, both vaginal and endocervical scrapes were taken from these women, and tested for HPV DNA by PCR, using MY09/MY11 primer system. HPV genotypes were analyzed by hybridizing PCR products with HPV type-specific biotinylated probes. HPV DNA was detected in both vaginal and cervical scrapes from the HPV-positive, but not from HPV-negative group. In the HPV-positive group, the same HPV type was found in vaginal and endocervical scrapes, giving a positive predictive value of 1.0. The results indicate that HPV types can be detected in vaginal scrapes, and recommend utilization of the less invasive vaginal testing for the routine detection of HPV DNA. |
| doi_str_mv | 10.1016/S1386-6532(00)00097-4 |
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The study group comprised 23 women tested positive and 28 women tested negative for cervical HPV DNA by PCR, and confirmed by histopathology. At the time of specimen collection, both vaginal and endocervical scrapes were taken from these women, and tested for HPV DNA by PCR, using MY09/MY11 primer system. HPV genotypes were analyzed by hybridizing PCR products with HPV type-specific biotinylated probes. HPV DNA was detected in both vaginal and cervical scrapes from the HPV-positive, but not from HPV-negative group. In the HPV-positive group, the same HPV type was found in vaginal and endocervical scrapes, giving a positive predictive value of 1.0. The results indicate that HPV types can be detected in vaginal scrapes, and recommend utilization of the less invasive vaginal testing for the routine detection of HPV DNA.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/S1386-6532(00)00097-4</identifier><identifier>PMID: 11090752</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adult ; Biological and medical sciences ; Cervical scrapes ; Cervix Uteri - virology ; DNA Probes ; DNA, Viral - analysis ; Female ; Genotype ; HPV ; Human papillomavirus ; Human viral diseases ; Humans ; Infectious diseases ; Medical sciences ; Pap smear ; Papillomaviridae - genetics ; Papillomaviridae - isolation & purification ; Papillomavirus Infections - virology ; PCR ; Polymerase Chain Reaction ; Reproducibility of Results ; Tumor Virus Infections - virology ; Vagina - virology ; Vaginal scrapes ; Viral diseases ; Viral diseases of the genital and urinary system</subject><ispartof>Journal of clinical virology, 2000-12, Vol.19 (3), p.163-168</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-402db394f64ab4ada516bd2be8350d3015e7074f0fa6407bb54df31bf71721443</citedby><cites>FETCH-LOGICAL-c467t-402db394f64ab4ada516bd2be8350d3015e7074f0fa6407bb54df31bf71721443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1386-6532(00)00097-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=824964$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11090752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Finan, Ramzi R</creatorcontrib><creatorcontrib>Irani-Hakime, Noha</creatorcontrib><creatorcontrib>Tamim, Hala</creatorcontrib><creatorcontrib>Almawi, Wassim Y</creatorcontrib><title>Validity of vaginal testing in detecting human papillomavirus (HPV) genotypes</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>The aim of this study was to assess the validity and usefulness of vaginal scrapes in detecting cervical human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR). The study group comprised 23 women tested positive and 28 women tested negative for cervical HPV DNA by PCR, and confirmed by histopathology. At the time of specimen collection, both vaginal and endocervical scrapes were taken from these women, and tested for HPV DNA by PCR, using MY09/MY11 primer system. HPV genotypes were analyzed by hybridizing PCR products with HPV type-specific biotinylated probes. HPV DNA was detected in both vaginal and cervical scrapes from the HPV-positive, but not from HPV-negative group. In the HPV-positive group, the same HPV type was found in vaginal and endocervical scrapes, giving a positive predictive value of 1.0. The results indicate that HPV types can be detected in vaginal scrapes, and recommend utilization of the less invasive vaginal testing for the routine detection of HPV DNA.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cervical scrapes</subject><subject>Cervix Uteri - virology</subject><subject>DNA Probes</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Genotype</subject><subject>HPV</subject><subject>Human papillomavirus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Pap smear</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomaviridae - isolation & purification</subject><subject>Papillomavirus Infections - virology</subject><subject>PCR</subject><subject>Polymerase Chain Reaction</subject><subject>Reproducibility of Results</subject><subject>Tumor Virus Infections - virology</subject><subject>Vagina - virology</subject><subject>Vaginal scrapes</subject><subject>Viral diseases</subject><subject>Viral diseases of the genital and urinary system</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNlKxTAQhoMo7o-gFATRi-pkb69ExA0UBZfbkDbJMdLNpD1w3t6eRb30ambgm5mfD6EDDGcYsDh_wTQTqeCUnACcAkAuU7aGtnEmacpzIdfH_gfZQjsxfgJgTpncRFsYQw6Sk230-K4rb3w_S1qXTPXEN7pKeht730wS3yTG9rZcDB9DrZuk052vqrbWUx-GmJzcPb-fJhPbtP2ss3EPbThdRbu_qrvo7eb69eoufXi6vb-6fEhLJmSfMiCmoDlzgumCaaM5FoUhhc0oB0PHmFaCZA6cFgxkUXBmHMWFk1gSzBjdRcfLu11ov4Yxrap9LG1V6ca2Q1SSMIqB5P-CWGYkyzgfQb4Ey9DGGKxTXfC1DjOFQc2Fq4VwNbepANRCuJonOVw9GIramr-tleEROFoBOpa6ckE3pY-_XEZYLuZnLpaUHa1NvQ0qlt42pTU-jP6Vaf0_Qb4BfYCbjA</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Finan, Ramzi R</creator><creator>Irani-Hakime, Noha</creator><creator>Tamim, Hala</creator><creator>Almawi, Wassim Y</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20001201</creationdate><title>Validity of vaginal testing in detecting human papillomavirus (HPV) genotypes</title><author>Finan, Ramzi R ; Irani-Hakime, Noha ; Tamim, Hala ; Almawi, Wassim Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-402db394f64ab4ada516bd2be8350d3015e7074f0fa6407bb54df31bf71721443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cervical scrapes</topic><topic>Cervix Uteri - virology</topic><topic>DNA Probes</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Genotype</topic><topic>HPV</topic><topic>Human papillomavirus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Pap smear</topic><topic>Papillomaviridae - genetics</topic><topic>Papillomaviridae - isolation & purification</topic><topic>Papillomavirus Infections - virology</topic><topic>PCR</topic><topic>Polymerase Chain Reaction</topic><topic>Reproducibility of Results</topic><topic>Tumor Virus Infections - virology</topic><topic>Vagina - virology</topic><topic>Vaginal scrapes</topic><topic>Viral diseases</topic><topic>Viral diseases of the genital and urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finan, Ramzi R</creatorcontrib><creatorcontrib>Irani-Hakime, Noha</creatorcontrib><creatorcontrib>Tamim, Hala</creatorcontrib><creatorcontrib>Almawi, Wassim Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finan, Ramzi R</au><au>Irani-Hakime, Noha</au><au>Tamim, Hala</au><au>Almawi, Wassim Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validity of vaginal testing in detecting human papillomavirus (HPV) genotypes</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>19</volume><issue>3</issue><spage>163</spage><epage>168</epage><pages>163-168</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>The aim of this study was to assess the validity and usefulness of vaginal scrapes in detecting cervical human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR). The study group comprised 23 women tested positive and 28 women tested negative for cervical HPV DNA by PCR, and confirmed by histopathology. At the time of specimen collection, both vaginal and endocervical scrapes were taken from these women, and tested for HPV DNA by PCR, using MY09/MY11 primer system. HPV genotypes were analyzed by hybridizing PCR products with HPV type-specific biotinylated probes. HPV DNA was detected in both vaginal and cervical scrapes from the HPV-positive, but not from HPV-negative group. In the HPV-positive group, the same HPV type was found in vaginal and endocervical scrapes, giving a positive predictive value of 1.0. 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| subjects | Adult Biological and medical sciences Cervical scrapes Cervix Uteri - virology DNA Probes DNA, Viral - analysis Female Genotype HPV Human papillomavirus Human viral diseases Humans Infectious diseases Medical sciences Pap smear Papillomaviridae - genetics Papillomaviridae - isolation & purification Papillomavirus Infections - virology PCR Polymerase Chain Reaction Reproducibility of Results Tumor Virus Infections - virology Vagina - virology Vaginal scrapes Viral diseases Viral diseases of the genital and urinary system |
| title | Validity of vaginal testing in detecting human papillomavirus (HPV) genotypes |
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