Automatic analysis for amperometrical recordings of exocytosis
Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA...
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| Veröffentlicht in: | Journal of neuroscience methods 2000-11, Vol.103 (2), p.151-156 |
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| creator | Segura, Fernando Brioso, Miguel A Gómez, José F Machado, J.David Borges, Ricardo |
| description | Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters; (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis. |
| doi_str_mv | 10.1016/S0165-0270(00)00309-5 |
| format | Article |
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The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters; (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/S0165-0270(00)00309-5</identifier><identifier>PMID: 11084207</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Action Potentials - physiology ; Adrenal Medulla - cytology ; Adrenal Medulla - metabolism ; Adrenaline ; Algorithms ; Animals ; Biological and medical sciences ; Catecholamines ; Catecholamines - metabolism ; Cattle ; Cell physiology ; Chromaffin cells ; Chromaffin Cells - cytology ; Chromaffin Cells - metabolism ; Digital filtering ; Electrophysiology - instrumentation ; Electrophysiology - methods ; Exocytosis - physiology ; Fundamental and applied biological sciences. Psychology ; IGOR macros ; Microelectrodes - standards ; Models, Neurological ; Molecular and cellular biology ; Secretion ; Secretion. Exocytosis ; Secretory Vesicles - metabolism ; Secretory Vesicles - ultrastructure ; Software - standards ; Spike analysis</subject><ispartof>Journal of neuroscience methods, 2000-11, Vol.103 (2), p.151-156</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ff8a25be8d1c649753ae9cb08f90b25efb72aaeb29019e6c774b8a5643a0a6133</citedby><cites>FETCH-LOGICAL-c389t-ff8a25be8d1c649753ae9cb08f90b25efb72aaeb29019e6c774b8a5643a0a6133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165027000003095$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=901053$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11084207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Segura, Fernando</creatorcontrib><creatorcontrib>Brioso, Miguel A</creatorcontrib><creatorcontrib>Gómez, José F</creatorcontrib><creatorcontrib>Machado, J.David</creatorcontrib><creatorcontrib>Borges, Ricardo</creatorcontrib><title>Automatic analysis for amperometrical recordings of exocytosis</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters; (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis.</description><subject>Action Potentials - physiology</subject><subject>Adrenal Medulla - cytology</subject><subject>Adrenal Medulla - metabolism</subject><subject>Adrenaline</subject><subject>Algorithms</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Catecholamines</subject><subject>Catecholamines - metabolism</subject><subject>Cattle</subject><subject>Cell physiology</subject><subject>Chromaffin cells</subject><subject>Chromaffin Cells - cytology</subject><subject>Chromaffin Cells - metabolism</subject><subject>Digital filtering</subject><subject>Electrophysiology - instrumentation</subject><subject>Electrophysiology - methods</subject><subject>Exocytosis - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IGOR macros</subject><subject>Microelectrodes - standards</subject><subject>Models, Neurological</subject><subject>Molecular and cellular biology</subject><subject>Secretion</subject><subject>Secretion. Exocytosis</subject><subject>Secretory Vesicles - metabolism</subject><subject>Secretory Vesicles - ultrastructure</subject><subject>Software - standards</subject><subject>Spike analysis</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF2r1DAQhoMo7p7Vn6AUBDleVCdN07Q3yrIcP2DBCxW8C9N0IpG2WZNW3H9_Uresl8Iwc_PMzMvD2DMOrznw6s2X1GQOhYJbgFcAAppcPmBbXqsir1T9_SHbXpENu4nxJwCUDVSP2YZzqMsC1Ja93c-TH3ByJsMR-3N0MbM-ZDicKPiBpuAM9lkg40Pnxh8x8zajP96cJ5_YJ-yRxT7S03Xu2Lf3d18PH_Pj5w-fDvtjbkTdTLm1NRaypbrjpiobJQVSY1qobQNtIcm2qkCktmiAN1QZpcq2RlmVAgErLsSOvbzcPQX_a6Y46cFFQ32PI_k5alWUAriQCZQX0AQfYyCrT8ENGM6ag17E6b_i9GJFw1JJnF72nq8P5nag7t_WaioBL1YAYzJiA47GxSuXgoNccr67UJRk_HYUdDSORkOdSwon3Xn3nyD3MN2KEw</recordid><startdate>20001130</startdate><enddate>20001130</enddate><creator>Segura, Fernando</creator><creator>Brioso, Miguel A</creator><creator>Gómez, José F</creator><creator>Machado, J.David</creator><creator>Borges, Ricardo</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001130</creationdate><title>Automatic analysis for amperometrical recordings of exocytosis</title><author>Segura, Fernando ; Brioso, Miguel A ; Gómez, José F ; Machado, J.David ; Borges, Ricardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ff8a25be8d1c649753ae9cb08f90b25efb72aaeb29019e6c774b8a5643a0a6133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Action Potentials - physiology</topic><topic>Adrenal Medulla - cytology</topic><topic>Adrenal Medulla - metabolism</topic><topic>Adrenaline</topic><topic>Algorithms</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Catecholamines</topic><topic>Catecholamines - metabolism</topic><topic>Cattle</topic><topic>Cell physiology</topic><topic>Chromaffin cells</topic><topic>Chromaffin Cells - cytology</topic><topic>Chromaffin Cells - metabolism</topic><topic>Digital filtering</topic><topic>Electrophysiology - instrumentation</topic><topic>Electrophysiology - methods</topic><topic>Exocytosis - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>IGOR macros</topic><topic>Microelectrodes - standards</topic><topic>Models, Neurological</topic><topic>Molecular and cellular biology</topic><topic>Secretion</topic><topic>Secretion. Exocytosis</topic><topic>Secretory Vesicles - metabolism</topic><topic>Secretory Vesicles - ultrastructure</topic><topic>Software - standards</topic><topic>Spike analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Segura, Fernando</creatorcontrib><creatorcontrib>Brioso, Miguel A</creatorcontrib><creatorcontrib>Gómez, José F</creatorcontrib><creatorcontrib>Machado, J.David</creatorcontrib><creatorcontrib>Borges, Ricardo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Segura, Fernando</au><au>Brioso, Miguel A</au><au>Gómez, José F</au><au>Machado, J.David</au><au>Borges, Ricardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automatic analysis for amperometrical recordings of exocytosis</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2000-11-30</date><risdate>2000</risdate><volume>103</volume><issue>2</issue><spage>151</spage><epage>156</epage><pages>151-156</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters; (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11084207</pmid><doi>10.1016/S0165-0270(00)00309-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Action Potentials - physiology Adrenal Medulla - cytology Adrenal Medulla - metabolism Adrenaline Algorithms Animals Biological and medical sciences Catecholamines Catecholamines - metabolism Cattle Cell physiology Chromaffin cells Chromaffin Cells - cytology Chromaffin Cells - metabolism Digital filtering Electrophysiology - instrumentation Electrophysiology - methods Exocytosis - physiology Fundamental and applied biological sciences. Psychology IGOR macros Microelectrodes - standards Models, Neurological Molecular and cellular biology Secretion Secretion. Exocytosis Secretory Vesicles - metabolism Secretory Vesicles - ultrastructure Software - standards Spike analysis |
| title | Automatic analysis for amperometrical recordings of exocytosis |
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