A 14‐3‐3 mRNA Is Up‐Regulated in Amyotrophic Lateral Sclerosis Spinal Cord
: We have recently isolated a 2.2‐kb cDNA clone (1C5) from ahuman spinal cord cDNA library with partial identity to the 14‐3‐3 proteinmRNA encoding the θ protein (YWHAQ). 14‐3‐3 protein transcripts arehighly expressed in large projection neurones of the hippocampus, cerebellum,and spinal cord and ha...
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| Veröffentlicht in: | Journal of neurochemistry 2000-12, Vol.75 (6), p.2511-2520 |
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| creator | Malaspina, Andrea Kaushik, Narendra Belleroche, Jackie de |
| description | : We have recently isolated a 2.2‐kb cDNA clone (1C5) from ahuman spinal cord cDNA library with partial identity to the 14‐3‐3 proteinmRNA encoding the θ protein (YWHAQ). 14‐3‐3 protein transcripts arehighly expressed in large projection neurones of the hippocampus, cerebellum,and spinal cord and have been found to be significantly up‐regulated in ratmotor neurones following hypoglossal nerve axotomy. In this study weinvestigated whether the 1C5 transcript (YWHAQ) isolated from spinal cord wasinvolved in amyotrophic lateral sclerosis (ALS). We found a significantup‐regulation of 1C5 (YWHAQ) in lumbar spinal cord from patients with sporadicALS compared with controls, with the highest levels of expression being foundin individuals with predominant lower motor neurone involvement. A 6‐bp tandemrepeat in the 5′‐untranslated region of the gene was found to bepolymorphic, but no significant association with disease was found followinggenomic analysis of this region. The localisation of 1C5 (YWHAQ) to chromosome2 was determined and coincides with that reported for clone HS1 (EMBLaccession no. X57347). These results show the marked up‐regulation of the14‐3‐3 isoform (YWHAQ) in ALS spinal cord and indicate the involvement of apotential 14‐3‐3‐mediated survival pathway in the pathogenesis of ALS. |
| doi_str_mv | 10.1046/j.1471-4159.2000.0752511.x |
| format | Article |
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In this study weinvestigated whether the 1C5 transcript (YWHAQ) isolated from spinal cord wasinvolved in amyotrophic lateral sclerosis (ALS). We found a significantup‐regulation of 1C5 (YWHAQ) in lumbar spinal cord from patients with sporadicALS compared with controls, with the highest levels of expression being foundin individuals with predominant lower motor neurone involvement. A 6‐bp tandemrepeat in the 5′‐untranslated region of the gene was found to bepolymorphic, but no significant association with disease was found followinggenomic analysis of this region. The localisation of 1C5 (YWHAQ) to chromosome2 was determined and coincides with that reported for clone HS1 (EMBLaccession no. X57347). These results show the marked up‐regulation of the14‐3‐3 isoform (YWHAQ) in ALS spinal cord and indicate the involvement of apotential 14‐3‐3‐mediated survival pathway in the pathogenesis of ALS.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.2000.0752511.x</identifier><identifier>PMID: 11080204</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>14-3-3 protein ; 14-3-3 Proteins ; 14‐3‐3 mRNA ; Adolescent ; Aged ; Aged, 80 and over ; Alleles ; Amino Acid Sequence ; Amyotrophic lateral sclerosis ; Amyotrophic Lateral Sclerosis - genetics ; Amyotrophic Lateral Sclerosis - metabolism ; Base Sequence ; Biological and medical sciences ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Female ; Frontal Lobe - chemistry ; Frontal Lobe - metabolism ; Frontal Lobe - pathology ; Gene Expression Profiling ; Humans ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; mRNA expression ; Neurology ; Organ Specificity - genetics ; Physical Chromosome Mapping ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; RNA, Messenger - metabolism ; Sequence Analysis, DNA ; Spinal cord ; Spinal Cord - chemistry ; Spinal Cord - metabolism ; Tandem Repeat Sequences - genetics ; Trinucleotide Repeats - genetics ; Tyrosine 3-Monooxygenase - genetics ; Tyrosine 3-Monooxygenase - metabolism</subject><ispartof>Journal of neurochemistry, 2000-12, Vol.75 (6), p.2511-2520</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5821-e1b8ccb3ea573477e6e285c3aeac4e6477f25845ce746ebd8ad728a2ff035363</citedby><cites>FETCH-LOGICAL-c5821-e1b8ccb3ea573477e6e285c3aeac4e6477f25845ce746ebd8ad728a2ff035363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.2000.0752511.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.2000.0752511.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=869577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11080204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malaspina, Andrea</creatorcontrib><creatorcontrib>Kaushik, Narendra</creatorcontrib><creatorcontrib>Belleroche, Jackie de</creatorcontrib><title>A 14‐3‐3 mRNA Is Up‐Regulated in Amyotrophic Lateral Sclerosis Spinal Cord</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: We have recently isolated a 2.2‐kb cDNA clone (1C5) from ahuman spinal cord cDNA library with partial identity to the 14‐3‐3 proteinmRNA encoding the θ protein (YWHAQ). 14‐3‐3 protein transcripts arehighly expressed in large projection neurones of the hippocampus, cerebellum,and spinal cord and have been found to be significantly up‐regulated in ratmotor neurones following hypoglossal nerve axotomy. In this study weinvestigated whether the 1C5 transcript (YWHAQ) isolated from spinal cord wasinvolved in amyotrophic lateral sclerosis (ALS). We found a significantup‐regulation of 1C5 (YWHAQ) in lumbar spinal cord from patients with sporadicALS compared with controls, with the highest levels of expression being foundin individuals with predominant lower motor neurone involvement. A 6‐bp tandemrepeat in the 5′‐untranslated region of the gene was found to bepolymorphic, but no significant association with disease was found followinggenomic analysis of this region. The localisation of 1C5 (YWHAQ) to chromosome2 was determined and coincides with that reported for clone HS1 (EMBLaccession no. X57347). These results show the marked up‐regulation of the14‐3‐3 isoform (YWHAQ) in ALS spinal cord and indicate the involvement of apotential 14‐3‐3‐mediated survival pathway in the pathogenesis of ALS.</description><subject>14-3-3 protein</subject><subject>14-3-3 Proteins</subject><subject>14‐3‐3 mRNA</subject><subject>Adolescent</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Amyotrophic lateral sclerosis</subject><subject>Amyotrophic Lateral Sclerosis - genetics</subject><subject>Amyotrophic Lateral Sclerosis - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Female</subject><subject>Frontal Lobe - chemistry</subject><subject>Frontal Lobe - metabolism</subject><subject>Frontal Lobe - pathology</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>mRNA expression</subject><subject>Neurology</subject><subject>Organ Specificity - genetics</subject><subject>Physical Chromosome Mapping</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Spinal cord</subject><subject>Spinal Cord - chemistry</subject><subject>Spinal Cord - metabolism</subject><subject>Tandem Repeat Sequences - genetics</subject><subject>Trinucleotide Repeats - genetics</subject><subject>Tyrosine 3-Monooxygenase - genetics</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkctO3DAUhq2qqAzQV6gsKrFLOL4HdtNRL1QjQFzWlsc5aTNKJqk9I5gdj9Bn5ElwNBFdoi4s6_z-zu9j_4QcM8gZSH26zJk0LJNMneUcAHIwiivG8sd3ZPJ69J5MADjPBEi-Tw5iXAIwLTX7QPYZgwI4yAm5nlImn5_-imHR9uZySi8ive9TeYO_No1bY0nrFZ22224duv537ek8icE19NY3GLpYR3rb16skzLpQHpG9yjURP477Ibn79vVu9iObX32_mE3nmVcFZxmyReH9QqBTRkhjUCMvlBcOnZeok1JxVUjl0UiNi7JwpeGF41UFQgktDsnJzrYP3Z8NxrVt6-ixadwKu020hkuuBfA3wfSBWgv5NshMGiAZJvB8B_r0-Biwsn2oWxe2loEdArJLO6RghxQGf7BjQPYxNX8ab9ksWiz_tY6JJODzCLjoXVMFt_J1fOUKfaaMSdSXHfVQN7j9jwHsz8vZWIgXQQisVQ</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Malaspina, Andrea</creator><creator>Kaushik, Narendra</creator><creator>Belleroche, Jackie de</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200012</creationdate><title>A 14‐3‐3 mRNA Is Up‐Regulated in Amyotrophic Lateral Sclerosis Spinal Cord</title><author>Malaspina, Andrea ; Kaushik, Narendra ; Belleroche, Jackie de</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5821-e1b8ccb3ea573477e6e285c3aeac4e6477f25845ce746ebd8ad728a2ff035363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>14-3-3 protein</topic><topic>14-3-3 Proteins</topic><topic>14‐3‐3 mRNA</topic><topic>Adolescent</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Amyotrophic lateral sclerosis</topic><topic>Amyotrophic Lateral Sclerosis - genetics</topic><topic>Amyotrophic Lateral Sclerosis - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Female</topic><topic>Frontal Lobe - chemistry</topic><topic>Frontal Lobe - metabolism</topic><topic>Frontal Lobe - pathology</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>mRNA expression</topic><topic>Neurology</topic><topic>Organ Specificity - genetics</topic><topic>Physical Chromosome Mapping</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Spinal cord</topic><topic>Spinal Cord - chemistry</topic><topic>Spinal Cord - metabolism</topic><topic>Tandem Repeat Sequences - genetics</topic><topic>Trinucleotide Repeats - genetics</topic><topic>Tyrosine 3-Monooxygenase - genetics</topic><topic>Tyrosine 3-Monooxygenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malaspina, Andrea</creatorcontrib><creatorcontrib>Kaushik, Narendra</creatorcontrib><creatorcontrib>Belleroche, Jackie de</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malaspina, Andrea</au><au>Kaushik, Narendra</au><au>Belleroche, Jackie de</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A 14‐3‐3 mRNA Is Up‐Regulated in Amyotrophic Lateral Sclerosis Spinal Cord</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2000-12</date><risdate>2000</risdate><volume>75</volume><issue>6</issue><spage>2511</spage><epage>2520</epage><pages>2511-2520</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: We have recently isolated a 2.2‐kb cDNA clone (1C5) from ahuman spinal cord cDNA library with partial identity to the 14‐3‐3 proteinmRNA encoding the θ protein (YWHAQ). 14‐3‐3 protein transcripts arehighly expressed in large projection neurones of the hippocampus, cerebellum,and spinal cord and have been found to be significantly up‐regulated in ratmotor neurones following hypoglossal nerve axotomy. In this study weinvestigated whether the 1C5 transcript (YWHAQ) isolated from spinal cord wasinvolved in amyotrophic lateral sclerosis (ALS). We found a significantup‐regulation of 1C5 (YWHAQ) in lumbar spinal cord from patients with sporadicALS compared with controls, with the highest levels of expression being foundin individuals with predominant lower motor neurone involvement. A 6‐bp tandemrepeat in the 5′‐untranslated region of the gene was found to bepolymorphic, but no significant association with disease was found followinggenomic analysis of this region. The localisation of 1C5 (YWHAQ) to chromosome2 was determined and coincides with that reported for clone HS1 (EMBLaccession no. X57347). These results show the marked up‐regulation of the14‐3‐3 isoform (YWHAQ) in ALS spinal cord and indicate the involvement of apotential 14‐3‐3‐mediated survival pathway in the pathogenesis of ALS.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11080204</pmid><doi>10.1046/j.1471-4159.2000.0752511.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 14-3-3 protein 14-3-3 Proteins 14‐3‐3 mRNA Adolescent Aged Aged, 80 and over Alleles Amino Acid Sequence Amyotrophic lateral sclerosis Amyotrophic Lateral Sclerosis - genetics Amyotrophic Lateral Sclerosis - metabolism Base Sequence Biological and medical sciences Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Female Frontal Lobe - chemistry Frontal Lobe - metabolism Frontal Lobe - pathology Gene Expression Profiling Humans Male Medical sciences Middle Aged Molecular Sequence Data mRNA expression Neurology Organ Specificity - genetics Physical Chromosome Mapping Protein Isoforms - genetics Protein Isoforms - metabolism RNA, Messenger - metabolism Sequence Analysis, DNA Spinal cord Spinal Cord - chemistry Spinal Cord - metabolism Tandem Repeat Sequences - genetics Trinucleotide Repeats - genetics Tyrosine 3-Monooxygenase - genetics Tyrosine 3-Monooxygenase - metabolism |
| title | A 14‐3‐3 mRNA Is Up‐Regulated in Amyotrophic Lateral Sclerosis Spinal Cord |
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