Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts
Myocardial dysfunction is a common and important problem in donor hearts. The mechanisms responsible remain unclear. We have studied the cytokines tumor necrosis factor (TNF)-alpha and interleukin-6 (IL-6) in the myocardium and serum from donors with myocardial dysfunction (unused donors) and compar...
Gespeichert in:
| Veröffentlicht in: | Circulation (New York, N.Y.) N.Y.), 2000-11, Vol.102 (19 Suppl 3), p.III352-III358 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | III358 |
|---|---|
| container_issue | 19 Suppl 3 |
| container_start_page | III352 |
| container_title | Circulation (New York, N.Y.) |
| container_volume | 102 |
| creator | Birks, E J Burton, P B Owen, V Mullen, A J Hunt, D Banner, N R Barton, P J Yacoub, M H |
| description | Myocardial dysfunction is a common and important problem in donor hearts. The mechanisms responsible remain unclear. We have studied the cytokines tumor necrosis factor (TNF)-alpha and interleukin-6 (IL-6) in the myocardium and serum from donors with myocardial dysfunction (unused donors) and compared them with donors with good ventricular function (used donors) and patients with advanced heart failure (HF).
Clinical details and ventricular function were assessed in 46 donors (31 used, 15 unused). Real-time reverse transcription-polymerase chain reaction, Western blotting, and immunocytochemistry were performed on myocardium and immunoassays on serum. TNF-alpha mRNA was 1.6-fold higher in unused than in used donors (P: |
| doi_str_mv | 10.1161/circ.102.suppl_3.III-352 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72424775</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72424775</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1583-d7d027e243a5436bba28699578b0cc30e3bd621fc13bdaec24a4ca3b490562213</originalsourceid><addsrcrecordid>eNpFkMtOwzAQRS0EoqXwC8grdgl-5rFEVYFIldjA2nIchxpiO9gJUv8eFyKxmrmauXc0BwCIUY5xge-VCSrHiORxHsdB0LxpmoxycgbWmBOWMU7rc7BGCNVZSQlZgasYP5IsaMkvwQpjVBGG6RrY3aC_5aQ7OM3WB-i0Cj6aCHupJh8yOYwHCaXroHGTDoOeP43LiqSgPXolQ2dm-zuPOqTO99DKoZ-dmox3xr3DzruUe9AyTPEaXPRyiPpmqRvw9rh73T5n-5enZvuwzxTmFc26skOk1IRRyRkt2laSqqhrXlYtUooiTduuILhXODVSK8IkU5K2rEa8IATTDbj7yx2D_5p1nIQ1UelhkE77OYqSMMLKkqfF6m_x9HUMuhdjMFaGo8BInFCLE-okiFhQi4RaJNTJervcmFuru3_jwpb-AGKxf_E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72424775</pqid></control><display><type>article</type><title>Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts</title><source>MEDLINE</source><source>American Heart Association Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Journals@Ovid Ovid Autoload</source><creator>Birks, E J ; Burton, P B ; Owen, V ; Mullen, A J ; Hunt, D ; Banner, N R ; Barton, P J ; Yacoub, M H</creator><creatorcontrib>Birks, E J ; Burton, P B ; Owen, V ; Mullen, A J ; Hunt, D ; Banner, N R ; Barton, P J ; Yacoub, M H</creatorcontrib><description><![CDATA[Myocardial dysfunction is a common and important problem in donor hearts. The mechanisms responsible remain unclear. We have studied the cytokines tumor necrosis factor (TNF)-alpha and interleukin-6 (IL-6) in the myocardium and serum from donors with myocardial dysfunction (unused donors) and compared them with donors with good ventricular function (used donors) and patients with advanced heart failure (HF).
Clinical details and ventricular function were assessed in 46 donors (31 used, 15 unused). Real-time reverse transcription-polymerase chain reaction, Western blotting, and immunocytochemistry were performed on myocardium and immunoassays on serum. TNF-alpha mRNA was 1.6-fold higher in unused than in used donors (P:<0.005) and 1.74-fold higher than in 36 patients with HF. IL-6 mRNA was 2.4-fold higher in unused than in used donors (P:<0.0001) and 4.67-fold higher than in HF (P:<0.0001). Western blotting showed higher TNF-alpha in unused (218. 3+/-6.4, n=4 versus 187.3+/-5.4, n=3 OD units) than used donors (P:<0.05). TNF-alpha expression was localized to cardiac myocytes. Serum TNF-alpha was higher in unused (8.72+/-1.3 pg/mL, n=13) than in used (6.12+/-0.8 pg/mL, n=25, P:<0.05) donors and HF (4.0+/-0.4 pg/mL, n=17, P:<0.005). Serum TNF-alpha receptors did not differ between unused (4.3+/-0.8 and 8.6+/-1.6 ng/mL, n=10) and used (3. 5+/-0.4 and 6.5+/-1.1 ng/mL, n=24) donors. There was a trend for higher serum IL-6 in unused (16.5+/-2.9 pg/mL, n=9) compared with used (13.9+/-1.6 pg/mL, n=26, P:=NS) donors.
This study documented an increase in the expression of TNF-alpha and IL-6 in the myocardium of all donor hearts that was more marked in the dysfunctional (unused) donor hearts. This was accompanied by similar changes in the serum. This might have important therapeutic implications.]]></description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/circ.102.suppl_3.III-352</identifier><identifier>PMID: 11082413</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Biomarkers - blood ; Blotting, Western ; Female ; Heart - physiopathology ; Heart Transplantation - standards ; Humans ; Immunohistochemistry ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Male ; Middle Aged ; Myocardium - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Tissue Donors - classification ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; Ventricular Dysfunction - blood ; Ventricular Dysfunction - diagnosis</subject><ispartof>Circulation (New York, N.Y.), 2000-11, Vol.102 (19 Suppl 3), p.III352-III358</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1583-d7d027e243a5436bba28699578b0cc30e3bd621fc13bdaec24a4ca3b490562213</citedby><cites>FETCH-LOGICAL-c1583-d7d027e243a5436bba28699578b0cc30e3bd621fc13bdaec24a4ca3b490562213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3674,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11082413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Birks, E J</creatorcontrib><creatorcontrib>Burton, P B</creatorcontrib><creatorcontrib>Owen, V</creatorcontrib><creatorcontrib>Mullen, A J</creatorcontrib><creatorcontrib>Hunt, D</creatorcontrib><creatorcontrib>Banner, N R</creatorcontrib><creatorcontrib>Barton, P J</creatorcontrib><creatorcontrib>Yacoub, M H</creatorcontrib><title>Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description><![CDATA[Myocardial dysfunction is a common and important problem in donor hearts. The mechanisms responsible remain unclear. We have studied the cytokines tumor necrosis factor (TNF)-alpha and interleukin-6 (IL-6) in the myocardium and serum from donors with myocardial dysfunction (unused donors) and compared them with donors with good ventricular function (used donors) and patients with advanced heart failure (HF).
Clinical details and ventricular function were assessed in 46 donors (31 used, 15 unused). Real-time reverse transcription-polymerase chain reaction, Western blotting, and immunocytochemistry were performed on myocardium and immunoassays on serum. TNF-alpha mRNA was 1.6-fold higher in unused than in used donors (P:<0.005) and 1.74-fold higher than in 36 patients with HF. IL-6 mRNA was 2.4-fold higher in unused than in used donors (P:<0.0001) and 4.67-fold higher than in HF (P:<0.0001). Western blotting showed higher TNF-alpha in unused (218. 3+/-6.4, n=4 versus 187.3+/-5.4, n=3 OD units) than used donors (P:<0.05). TNF-alpha expression was localized to cardiac myocytes. Serum TNF-alpha was higher in unused (8.72+/-1.3 pg/mL, n=13) than in used (6.12+/-0.8 pg/mL, n=25, P:<0.05) donors and HF (4.0+/-0.4 pg/mL, n=17, P:<0.005). Serum TNF-alpha receptors did not differ between unused (4.3+/-0.8 and 8.6+/-1.6 ng/mL, n=10) and used (3. 5+/-0.4 and 6.5+/-1.1 ng/mL, n=24) donors. There was a trend for higher serum IL-6 in unused (16.5+/-2.9 pg/mL, n=9) compared with used (13.9+/-1.6 pg/mL, n=26, P:=NS) donors.
This study documented an increase in the expression of TNF-alpha and IL-6 in the myocardium of all donor hearts that was more marked in the dysfunctional (unused) donor hearts. This was accompanied by similar changes in the serum. This might have important therapeutic implications.]]></description><subject>Adult</subject><subject>Biomarkers - blood</subject><subject>Blotting, Western</subject><subject>Female</subject><subject>Heart - physiopathology</subject><subject>Heart Transplantation - standards</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myocardium - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Tissue Donors - classification</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Ventricular Dysfunction - blood</subject><subject>Ventricular Dysfunction - diagnosis</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtOwzAQRS0EoqXwC8grdgl-5rFEVYFIldjA2nIchxpiO9gJUv8eFyKxmrmauXc0BwCIUY5xge-VCSrHiORxHsdB0LxpmoxycgbWmBOWMU7rc7BGCNVZSQlZgasYP5IsaMkvwQpjVBGG6RrY3aC_5aQ7OM3WB-i0Cj6aCHupJh8yOYwHCaXroHGTDoOeP43LiqSgPXolQ2dm-zuPOqTO99DKoZ-dmox3xr3DzruUe9AyTPEaXPRyiPpmqRvw9rh73T5n-5enZvuwzxTmFc26skOk1IRRyRkt2laSqqhrXlYtUooiTduuILhXODVSK8IkU5K2rEa8IATTDbj7yx2D_5p1nIQ1UelhkE77OYqSMMLKkqfF6m_x9HUMuhdjMFaGo8BInFCLE-okiFhQi4RaJNTJervcmFuru3_jwpb-AGKxf_E</recordid><startdate>20001107</startdate><enddate>20001107</enddate><creator>Birks, E J</creator><creator>Burton, P B</creator><creator>Owen, V</creator><creator>Mullen, A J</creator><creator>Hunt, D</creator><creator>Banner, N R</creator><creator>Barton, P J</creator><creator>Yacoub, M H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001107</creationdate><title>Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts</title><author>Birks, E J ; Burton, P B ; Owen, V ; Mullen, A J ; Hunt, D ; Banner, N R ; Barton, P J ; Yacoub, M H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1583-d7d027e243a5436bba28699578b0cc30e3bd621fc13bdaec24a4ca3b490562213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Biomarkers - blood</topic><topic>Blotting, Western</topic><topic>Female</topic><topic>Heart - physiopathology</topic><topic>Heart Transplantation - standards</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myocardium - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Tissue Donors - classification</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Ventricular Dysfunction - blood</topic><topic>Ventricular Dysfunction - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Birks, E J</creatorcontrib><creatorcontrib>Burton, P B</creatorcontrib><creatorcontrib>Owen, V</creatorcontrib><creatorcontrib>Mullen, A J</creatorcontrib><creatorcontrib>Hunt, D</creatorcontrib><creatorcontrib>Banner, N R</creatorcontrib><creatorcontrib>Barton, P J</creatorcontrib><creatorcontrib>Yacoub, M H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Birks, E J</au><au>Burton, P B</au><au>Owen, V</au><au>Mullen, A J</au><au>Hunt, D</au><au>Banner, N R</au><au>Barton, P J</au><au>Yacoub, M H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>2000-11-07</date><risdate>2000</risdate><volume>102</volume><issue>19 Suppl 3</issue><spage>III352</spage><epage>III358</epage><pages>III352-III358</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><abstract><![CDATA[Myocardial dysfunction is a common and important problem in donor hearts. The mechanisms responsible remain unclear. We have studied the cytokines tumor necrosis factor (TNF)-alpha and interleukin-6 (IL-6) in the myocardium and serum from donors with myocardial dysfunction (unused donors) and compared them with donors with good ventricular function (used donors) and patients with advanced heart failure (HF).
Clinical details and ventricular function were assessed in 46 donors (31 used, 15 unused). Real-time reverse transcription-polymerase chain reaction, Western blotting, and immunocytochemistry were performed on myocardium and immunoassays on serum. TNF-alpha mRNA was 1.6-fold higher in unused than in used donors (P:<0.005) and 1.74-fold higher than in 36 patients with HF. IL-6 mRNA was 2.4-fold higher in unused than in used donors (P:<0.0001) and 4.67-fold higher than in HF (P:<0.0001). Western blotting showed higher TNF-alpha in unused (218. 3+/-6.4, n=4 versus 187.3+/-5.4, n=3 OD units) than used donors (P:<0.05). TNF-alpha expression was localized to cardiac myocytes. Serum TNF-alpha was higher in unused (8.72+/-1.3 pg/mL, n=13) than in used (6.12+/-0.8 pg/mL, n=25, P:<0.05) donors and HF (4.0+/-0.4 pg/mL, n=17, P:<0.005). Serum TNF-alpha receptors did not differ between unused (4.3+/-0.8 and 8.6+/-1.6 ng/mL, n=10) and used (3. 5+/-0.4 and 6.5+/-1.1 ng/mL, n=24) donors. There was a trend for higher serum IL-6 in unused (16.5+/-2.9 pg/mL, n=9) compared with used (13.9+/-1.6 pg/mL, n=26, P:=NS) donors.
This study documented an increase in the expression of TNF-alpha and IL-6 in the myocardium of all donor hearts that was more marked in the dysfunctional (unused) donor hearts. This was accompanied by similar changes in the serum. This might have important therapeutic implications.]]></abstract><cop>United States</cop><pmid>11082413</pmid><doi>10.1161/circ.102.suppl_3.III-352</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-7322 |
| ispartof | Circulation (New York, N.Y.), 2000-11, Vol.102 (19 Suppl 3), p.III352-III358 |
| issn | 0009-7322 1524-4539 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72424775 |
| source | MEDLINE; American Heart Association Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Ovid Autoload |
| subjects | Adult Biomarkers - blood Blotting, Western Female Heart - physiopathology Heart Transplantation - standards Humans Immunohistochemistry Interleukin-6 - genetics Interleukin-6 - metabolism Male Middle Aged Myocardium - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Tissue Donors - classification Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Ventricular Dysfunction - blood Ventricular Dysfunction - diagnosis |
| title | Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T13%3A09%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Elevated%20tumor%20necrosis%20factor-alpha%20and%20interleukin-6%20in%20myocardium%20and%20serum%20of%20malfunctioning%20donor%20hearts&rft.jtitle=Circulation%20(New%20York,%20N.Y.)&rft.au=Birks,%20E%20J&rft.date=2000-11-07&rft.volume=102&rft.issue=19%20Suppl%203&rft.spage=III352&rft.epage=III358&rft.pages=III352-III358&rft.issn=0009-7322&rft.eissn=1524-4539&rft_id=info:doi/10.1161/circ.102.suppl_3.III-352&rft_dat=%3Cproquest_cross%3E72424775%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72424775&rft_id=info:pmid/11082413&rfr_iscdi=true |