Mutational and comparative analysis of streptolysin O, an oxygen-labile streptococcal hemolysin

The structural gene of streptolysin O was cloned from Streptococcus pyogenes strain Sa and S. equisimilis H46A, and the nucleotide sequences were compared with those of strain Richards. To obtain the minimal active fragment of the toxin and to elucidate structure-function relationships in hemolytic...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-12, Vol.65 (12), p.2682-2689
Hauptverfasser: Yamamoto, I. (Fukui Medical School, Matsuoka (Japan)), Kimoto, H, Taketo, Y, Taketo, A
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creator Yamamoto, I. (Fukui Medical School, Matsuoka (Japan))
Kimoto, H
Taketo, Y
Taketo, A
description The structural gene of streptolysin O was cloned from Streptococcus pyogenes strain Sa and S. equisimilis H46A, and the nucleotide sequences were compared with those of strain Richards. To obtain the minimal active fragment of the toxin and to elucidate structure-function relationships in hemolytic function, streptolysin O mutants deleted in N- and C-terminal regions were constructed. Internal amino acid residues were also replaced by introduction of point mutations. Analyses of these mutants showed that considerable activity was retained even after deletion of the N-terminal 107 residues, but genetic removal of the ultimate C-terminal residue resulted in a marked decrease in hemolytic function. By removal in succession, hemolytic activity declined exponentially, and only 0.002% of the activity remained after deletion of the C-terminal four residues. Nucleotide replacement experiments indicated pivotal roles of I202, V217, D324-L325, V339, and H469 residues in hemolysis.
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source J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry
subjects Amino Acid Sequence
Animals
Bacterial Proteins
Base Sequence
Biological and medical sciences
Biotechnology
DNA Primers
Fundamental and applied biological sciences. Psychology
GENES
Genes, Bacterial
HAEMOLYSIS
hemolysin
Hemolysin Proteins - metabolism
MOLECULAR CLONING
Molecular Sequence Data
MUTATION
mutational analysis
NUCLEOTIDE SEQUENCE
Oxygen - metabolism
Plasmids
Point Mutation
Sheep
STREPTOCOCCUS
Streptococcus pyogenes - genetics
Streptococcus pyogenes - metabolism
streptolysin O
Streptolysins - chemistry
Streptolysins - genetics
Streptolysins - metabolism
title Mutational and comparative analysis of streptolysin O, an oxygen-labile streptococcal hemolysin
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