Comparative physical mapping of the 18S-5.8S-26S rDNA in three sorghum species
The physical locations of the 18S-5.8S-26S rDNA sequences were examined in three sorghum species by fluorescence in situ hybridization (FISH) using biotin-labeled heterologous 18S-5.8S-26S rDNA probe (pTa71). Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2...
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| Veröffentlicht in: | Genome 2000-10, Vol.43 (5), p.918-922 |
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| creator | Sang, Y Liang, G.H |
| description | The physical locations of the 18S-5.8S-26S rDNA sequences were examined in three sorghum species by fluorescence in situ hybridization (FISH) using biotin-labeled heterologous 18S-5.8S-26S rDNA probe (pTa71). Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2n = 20) and S. versicolor (2n = 10), but at four sites on the chromosomes of S. halepense (2n = 40) and the tetraploid S. versicolor (2n = 20). Positions of the rDNA loci varied from the interstitial to terminal position among the four accessions of the three sorghum species. The rDNA data are useful for investigation of chromosome evolution and phylogeny. This study excluded S. versicolor as the possible progenitor of S. bicolor.Key words: Sorghum bicolor, Sorghum versicolor, Sorghum halepense, 18S-5.8S-26S rDNA, fluorescence in situ hybridization. |
| doi_str_mv | 10.1139/g00-063 |
| format | Article |
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Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2n = 20) and S. versicolor (2n = 10), but at four sites on the chromosomes of S. halepense (2n = 40) and the tetraploid S. versicolor (2n = 20). Positions of the rDNA loci varied from the interstitial to terminal position among the four accessions of the three sorghum species. The rDNA data are useful for investigation of chromosome evolution and phylogeny. This study excluded S. versicolor as the possible progenitor of S. bicolor.Key words: Sorghum bicolor, Sorghum versicolor, Sorghum halepense, 18S-5.8S-26S rDNA, fluorescence in situ hybridization.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g00-063</identifier><identifier>PMID: 11081984</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>chromosome mapping ; chromosomes ; DNA, Ribosomal ; Edible Grain - genetics ; fluorescence ; fluorescence in situ hybridization ; gene location ; In Situ Hybridization, Fluorescence ; loci ; Mitosis ; nucleic acid hybridization ; nucleotide sequences ; phylogeny ; Physical Chromosome Mapping - methods ; Ploidies ; ribosomal DNA ; rRNA 18S ; rRNA 26S ; rRNA 5.8S ; Sorghum bicolor ; Sorghum halepense ; Sorghum versicolor ; species differences</subject><ispartof>Genome, 2000-10, Vol.43 (5), p.918-922</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-59b932b5f289f6850d5e7f58c9c30c694340d947d855f671154e032d86aee3f33</citedby><cites>FETCH-LOGICAL-c313t-59b932b5f289f6850d5e7f58c9c30c694340d947d855f671154e032d86aee3f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g00-063$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g00-063$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,780,784,2930,27923,27924,64427,65005</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11081984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sang, Y</creatorcontrib><creatorcontrib>Liang, G.H</creatorcontrib><title>Comparative physical mapping of the 18S-5.8S-26S rDNA in three sorghum species</title><title>Genome</title><addtitle>Génome</addtitle><description>The physical locations of the 18S-5.8S-26S rDNA sequences were examined in three sorghum species by fluorescence in situ hybridization (FISH) using biotin-labeled heterologous 18S-5.8S-26S rDNA probe (pTa71). Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2n = 20) and S. versicolor (2n = 10), but at four sites on the chromosomes of S. halepense (2n = 40) and the tetraploid S. versicolor (2n = 20). Positions of the rDNA loci varied from the interstitial to terminal position among the four accessions of the three sorghum species. The rDNA data are useful for investigation of chromosome evolution and phylogeny. This study excluded S. versicolor as the possible progenitor of S. bicolor.Key words: Sorghum bicolor, Sorghum versicolor, Sorghum halepense, 18S-5.8S-26S rDNA, fluorescence in situ hybridization.</description><subject>chromosome mapping</subject><subject>chromosomes</subject><subject>DNA, Ribosomal</subject><subject>Edible Grain - genetics</subject><subject>fluorescence</subject><subject>fluorescence in situ hybridization</subject><subject>gene location</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>loci</subject><subject>Mitosis</subject><subject>nucleic acid hybridization</subject><subject>nucleotide sequences</subject><subject>phylogeny</subject><subject>Physical Chromosome Mapping - methods</subject><subject>Ploidies</subject><subject>ribosomal DNA</subject><subject>rRNA 18S</subject><subject>rRNA 26S</subject><subject>rRNA 5.8S</subject><subject>Sorghum bicolor</subject><subject>Sorghum halepense</subject><subject>Sorghum versicolor</subject><subject>species differences</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFuEzEQBmALgWhaEG8APoGEtO2MZ-21j1WgFKkqh9Cz5XjHyaJsdrGTSn17tkpEDwj1MnOYT_9IvxDvEM4RyV2sACow9ELMsLZQESl8KWZgCSvVOHMiTkv5BYBADl-LE0Sw6Gw9E7fzoR9DDrvunuW4fihdDBvZh3Hstis5JLlbs0S7qPT5NJRZyPzl9lJ22-mQmWUZ8mq972UZOXZc3ohXKWwKvz3uM3F39fXn_Lq6-fHt-_zypoqEtKu0WzpSS52UdclYDa3mJmkbXSSIxtVUQ-vqprVaJ9Mg6pqBVGtNYKZEdCY-HnLHPPzec9n5viuRN5uw5WFffKNqBWTgWYiNUWZyE_x0gDEPpWROfsxdH_KDR_CPHfupYz91PMn3x8j9suf2yR1Lffq5zTFz4ZDj-q86pvixTRP8_H_479sPB5zC4MMqd8XfLRQggXKqbkDTH-ksl2E</recordid><startdate>200010</startdate><enddate>200010</enddate><creator>Sang, Y</creator><creator>Liang, G.H</creator><general>NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200010</creationdate><title>Comparative physical mapping of the 18S-5.8S-26S rDNA in three sorghum species</title><author>Sang, Y ; Liang, G.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-59b932b5f289f6850d5e7f58c9c30c694340d947d855f671154e032d86aee3f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>chromosome mapping</topic><topic>chromosomes</topic><topic>DNA, Ribosomal</topic><topic>Edible Grain - genetics</topic><topic>fluorescence</topic><topic>fluorescence in situ hybridization</topic><topic>gene location</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>loci</topic><topic>Mitosis</topic><topic>nucleic acid hybridization</topic><topic>nucleotide sequences</topic><topic>phylogeny</topic><topic>Physical Chromosome Mapping - methods</topic><topic>Ploidies</topic><topic>ribosomal DNA</topic><topic>rRNA 18S</topic><topic>rRNA 26S</topic><topic>rRNA 5.8S</topic><topic>Sorghum bicolor</topic><topic>Sorghum halepense</topic><topic>Sorghum versicolor</topic><topic>species differences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sang, Y</creatorcontrib><creatorcontrib>Liang, G.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sang, Y</au><au>Liang, G.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative physical mapping of the 18S-5.8S-26S rDNA in three sorghum species</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2000-10</date><risdate>2000</risdate><volume>43</volume><issue>5</issue><spage>918</spage><epage>922</epage><pages>918-922</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><abstract>The physical locations of the 18S-5.8S-26S rDNA sequences were examined in three sorghum species by fluorescence in situ hybridization (FISH) using biotin-labeled heterologous 18S-5.8S-26S rDNA probe (pTa71). Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2n = 20) and S. versicolor (2n = 10), but at four sites on the chromosomes of S. halepense (2n = 40) and the tetraploid S. versicolor (2n = 20). Positions of the rDNA loci varied from the interstitial to terminal position among the four accessions of the three sorghum species. The rDNA data are useful for investigation of chromosome evolution and phylogeny. This study excluded S. versicolor as the possible progenitor of S. bicolor.Key words: Sorghum bicolor, Sorghum versicolor, Sorghum halepense, 18S-5.8S-26S rDNA, fluorescence in situ hybridization.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>11081984</pmid><doi>10.1139/g00-063</doi><tpages>5</tpages></addata></record> |
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| subjects | chromosome mapping chromosomes DNA, Ribosomal Edible Grain - genetics fluorescence fluorescence in situ hybridization gene location In Situ Hybridization, Fluorescence loci Mitosis nucleic acid hybridization nucleotide sequences phylogeny Physical Chromosome Mapping - methods Ploidies ribosomal DNA rRNA 18S rRNA 26S rRNA 5.8S Sorghum bicolor Sorghum halepense Sorghum versicolor species differences |
| title | Comparative physical mapping of the 18S-5.8S-26S rDNA in three sorghum species |
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