Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test

The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Metabolism, clinical and experimental clinical and experimental, 2000-10, Vol.49 (10), p.1352-1355
Hauptverfasser: Goi, Giancarlo, Bairati, Chiara, Burlina, Alberto, Massaccesi, Luca, Monciotti, Carla, Segalini, Giovanni, Testa, Roberto, Lombardo, Adriana
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1355
container_issue 10
container_start_page 1352
container_title Metabolism, clinical and experimental
container_volume 49
creator Goi, Giancarlo
Bairati, Chiara
Burlina, Alberto
Massaccesi, Luca
Monciotti, Carla
Segalini, Giovanni
Testa, Roberto
Lombardo, Adriana
description The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). Glycohydrolase abnormalities appear to be related to insulin deficiency rather than hyperglycemia. Lysosomal apparatus abnormalities seem to be an inherent feature of diabetes that is present at disease onset. The possible role of insulin in the regulation of plasma glycohydrolase levels is discussed.
doi_str_mv 10.1053/meta.2000.9507
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72418404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0026049500805326</els_id><sourcerecordid>72418404</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-2f644bcedce88ecb19eb30851b10ed6fc8194c817057fad18ec8c0b6607d43bb3</originalsourceid><addsrcrecordid>eNp1kUtv1DAQgC0EotvClSOyhMQt23GezhFVBSpVgkN7tvyYbF0l8WI7i_YX9G8z0a7gxMUPzed5fGbsg4CtgKa6njDrbQkA276B7hXbiKYqC9kCvGYbgLItoO6bC3aZ0jNRXSfbt-xCCOh6WcoNe_k56jRpvhuPNjwdXQx0Rz7iAcfE_cz3Onucc-K_fX7i-bhHLrjz2mDGxHXmYU6YuZ7dSqfFPKMlepkdxl3w845CFMlRH3AOS6JKiw1UIocRo54tnTDld-zNoMeE78_7FXv8evtw8724__Ht7ubLfWGrVuaiHNq6NhadRSnRGtGjqUA2wghA1w5Wir6mpYOmG7QTxEgLpm2hc3VlTHXFPp_y7mP4tVBhNflkcRz1jNSd6spayBpqArcn0MaQUsRB7aOfdDwqAWpVr1b1alWvVvX04OM582ImdP_ws2sCPp0Bnaweh3V4n_5yEtpKCKLkiSL_ePAYVbL0ATSyj2RWueD_18EfNDSi1A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72418404</pqid></control><display><type>article</type><title>Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Goi, Giancarlo ; Bairati, Chiara ; Burlina, Alberto ; Massaccesi, Luca ; Monciotti, Carla ; Segalini, Giovanni ; Testa, Roberto ; Lombardo, Adriana</creator><creatorcontrib>Goi, Giancarlo ; Bairati, Chiara ; Burlina, Alberto ; Massaccesi, Luca ; Monciotti, Carla ; Segalini, Giovanni ; Testa, Roberto ; Lombardo, Adriana</creatorcontrib><description>The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). Glycohydrolase abnormalities appear to be related to insulin deficiency rather than hyperglycemia. Lysosomal apparatus abnormalities seem to be an inherent feature of diabetes that is present at disease onset. The possible role of insulin in the regulation of plasma glycohydrolase levels is discussed.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1053/meta.2000.9507</identifier><identifier>PMID: 11079828</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adolescent ; Biological and medical sciences ; Blood Glucose - analysis ; Child ; Child, Preschool ; Diabetes Mellitus, Type 1 - enzymology ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Glucose Tolerance Test ; Glycoside Hydrolases - blood ; Humans ; Insulin - blood ; Lysosomes - enzymology ; Medical sciences</subject><ispartof>Metabolism, clinical and experimental, 2000-10, Vol.49 (10), p.1352-1355</ispartof><rights>2000 Academic Press</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-2f644bcedce88ecb19eb30851b10ed6fc8194c817057fad18ec8c0b6607d43bb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1053/meta.2000.9507$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=806311$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11079828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goi, Giancarlo</creatorcontrib><creatorcontrib>Bairati, Chiara</creatorcontrib><creatorcontrib>Burlina, Alberto</creatorcontrib><creatorcontrib>Massaccesi, Luca</creatorcontrib><creatorcontrib>Monciotti, Carla</creatorcontrib><creatorcontrib>Segalini, Giovanni</creatorcontrib><creatorcontrib>Testa, Roberto</creatorcontrib><creatorcontrib>Lombardo, Adriana</creatorcontrib><title>Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). Glycohydrolase abnormalities appear to be related to insulin deficiency rather than hyperglycemia. Lysosomal apparatus abnormalities seem to be an inherent feature of diabetes that is present at disease onset. The possible role of insulin in the regulation of plasma glycohydrolase levels is discussed.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - analysis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Diabetes Mellitus, Type 1 - enzymology</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Glucose Tolerance Test</subject><subject>Glycoside Hydrolases - blood</subject><subject>Humans</subject><subject>Insulin - blood</subject><subject>Lysosomes - enzymology</subject><subject>Medical sciences</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1DAQgC0EotvClSOyhMQt23GezhFVBSpVgkN7tvyYbF0l8WI7i_YX9G8z0a7gxMUPzed5fGbsg4CtgKa6njDrbQkA276B7hXbiKYqC9kCvGYbgLItoO6bC3aZ0jNRXSfbt-xCCOh6WcoNe_k56jRpvhuPNjwdXQx0Rz7iAcfE_cz3Onucc-K_fX7i-bhHLrjz2mDGxHXmYU6YuZ7dSqfFPKMlepkdxl3w845CFMlRH3AOS6JKiw1UIocRo54tnTDld-zNoMeE78_7FXv8evtw8724__Ht7ubLfWGrVuaiHNq6NhadRSnRGtGjqUA2wghA1w5Wir6mpYOmG7QTxEgLpm2hc3VlTHXFPp_y7mP4tVBhNflkcRz1jNSd6spayBpqArcn0MaQUsRB7aOfdDwqAWpVr1b1alWvVvX04OM582ImdP_ws2sCPp0Bnaweh3V4n_5yEtpKCKLkiSL_ePAYVbL0ATSyj2RWueD_18EfNDSi1A</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Goi, Giancarlo</creator><creator>Bairati, Chiara</creator><creator>Burlina, Alberto</creator><creator>Massaccesi, Luca</creator><creator>Monciotti, Carla</creator><creator>Segalini, Giovanni</creator><creator>Testa, Roberto</creator><creator>Lombardo, Adriana</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test</title><author>Goi, Giancarlo ; Bairati, Chiara ; Burlina, Alberto ; Massaccesi, Luca ; Monciotti, Carla ; Segalini, Giovanni ; Testa, Roberto ; Lombardo, Adriana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-2f644bcedce88ecb19eb30851b10ed6fc8194c817057fad18ec8c0b6607d43bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - analysis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Diabetes Mellitus, Type 1 - enzymology</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Glucose Tolerance Test</topic><topic>Glycoside Hydrolases - blood</topic><topic>Humans</topic><topic>Insulin - blood</topic><topic>Lysosomes - enzymology</topic><topic>Medical sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goi, Giancarlo</creatorcontrib><creatorcontrib>Bairati, Chiara</creatorcontrib><creatorcontrib>Burlina, Alberto</creatorcontrib><creatorcontrib>Massaccesi, Luca</creatorcontrib><creatorcontrib>Monciotti, Carla</creatorcontrib><creatorcontrib>Segalini, Giovanni</creatorcontrib><creatorcontrib>Testa, Roberto</creatorcontrib><creatorcontrib>Lombardo, Adriana</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goi, Giancarlo</au><au>Bairati, Chiara</au><au>Burlina, Alberto</au><au>Massaccesi, Luca</au><au>Monciotti, Carla</au><au>Segalini, Giovanni</au><au>Testa, Roberto</au><au>Lombardo, Adriana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>49</volume><issue>10</issue><spage>1352</spage><epage>1355</epage><pages>1352-1355</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). Glycohydrolase abnormalities appear to be related to insulin deficiency rather than hyperglycemia. Lysosomal apparatus abnormalities seem to be an inherent feature of diabetes that is present at disease onset. The possible role of insulin in the regulation of plasma glycohydrolase levels is discussed.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11079828</pmid><doi>10.1053/meta.2000.9507</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0026-0495
ispartof Metabolism, clinical and experimental, 2000-10, Vol.49 (10), p.1352-1355
issn 0026-0495
1532-8600
language eng
recordid cdi_proquest_miscellaneous_72418404
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Adolescent
Biological and medical sciences
Blood Glucose - analysis
Child
Child, Preschool
Diabetes Mellitus, Type 1 - enzymology
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Glucose Tolerance Test
Glycoside Hydrolases - blood
Humans
Insulin - blood
Lysosomes - enzymology
Medical sciences
title Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T15%3A11%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20glycohydrolase%20levels%20in%20patients%20with%20type%201%20diabetes%20at%20onset%20and%20in%20subjects%20undergoing%20an%20intravenous%20glucose%20tolerance%20test&rft.jtitle=Metabolism,%20clinical%20and%20experimental&rft.au=Goi,%20Giancarlo&rft.date=2000-10-01&rft.volume=49&rft.issue=10&rft.spage=1352&rft.epage=1355&rft.pages=1352-1355&rft.issn=0026-0495&rft.eissn=1532-8600&rft_id=info:doi/10.1053/meta.2000.9507&rft_dat=%3Cproquest_cross%3E72418404%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72418404&rft_id=info:pmid/11079828&rft_els_id=S0026049500805326&rfr_iscdi=true