Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test

The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (...

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Veröffentlicht in:Metabolism, clinical and experimental clinical and experimental, 2000-10, Vol.49 (10), p.1352-1355
Hauptverfasser: Goi, Giancarlo, Bairati, Chiara, Burlina, Alberto, Massaccesi, Luca, Monciotti, Carla, Segalini, Giovanni, Testa, Roberto, Lombardo, Adriana
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container_end_page 1355
container_issue 10
container_start_page 1352
container_title Metabolism, clinical and experimental
container_volume 49
creator Goi, Giancarlo
Bairati, Chiara
Burlina, Alberto
Massaccesi, Luca
Monciotti, Carla
Segalini, Giovanni
Testa, Roberto
Lombardo, Adriana
description The effect of hyperglycemia and insulin deficiency on the plasma level of lysosomal glycohydrolases, namely N-acetyl-β- D-glucosaminidase, β- D-glucuronidase, α- D-galactosidase, and α- D-glucosidase, was investigated. Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). Glycohydrolase abnormalities appear to be related to insulin deficiency rather than hyperglycemia. Lysosomal apparatus abnormalities seem to be an inherent feature of diabetes that is present at disease onset. The possible role of insulin in the regulation of plasma glycohydrolase levels is discussed.
doi_str_mv 10.1053/meta.2000.9507
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Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). 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Two patient groups were assessed: (1) 28 children with type 1 diabetes at onset (fasting blood glucose, 444 ± 154 mg/100 mL; hemoglobin A 1c, 11.9% ± 2.4%; symptom duration, 15.9 ± 8 days; and absence of complications), (2) 14 adult subjects undergoing an intravenous glucose tolerance test (IVGTT), consisting of 8 non-obese subjects (body mass index, 26 ± 0.04 kg/m 2; fasting blood glucose, 82 ± 13 mg/100 mL; blood insulin, 6 ± 0.04 mU/L) and 6 obese subjects (fasting blood glucose, 97 ± 3.5 mg/100 mL; blood insulin, 27 ± 6 mU/L, with normal oral glucose tolerance test). Enzyme activity was determined with the fluorimetric method. The mean level of all evaluated enzymes was significantly increased in patients with type 1 diabetes at diagnosis compared with normal controls. Increased enzyme levels were also detected in the group of adults undergoing an IVGTT in whom hyperglycemia was accompanied by insulin resistance (ie, obese subjects). 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Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. 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Glycohydrolase abnormalities appear to be related to insulin deficiency rather than hyperglycemia. Lysosomal apparatus abnormalities seem to be an inherent feature of diabetes that is present at disease onset. The possible role of insulin in the regulation of plasma glycohydrolase levels is discussed.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11079828</pmid><doi>10.1053/meta.2000.9507</doi><tpages>4</tpages></addata></record>
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subjects Adolescent
Biological and medical sciences
Blood Glucose - analysis
Child
Child, Preschool
Diabetes Mellitus, Type 1 - enzymology
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Glucose Tolerance Test
Glycoside Hydrolases - blood
Humans
Insulin - blood
Lysosomes - enzymology
Medical sciences
title Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test
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