Cloning of Plasmodium falciparum protein disulfide isomerase homologue by affinity purification using the antiplasmodial inhibitor 1,4-bis[3-[N-(cyclohexyl methyl)amino]propyl]piperazine
A series of 10 1,4-bis(3-aminopropyl)piperazine compounds was found to display antiplasmodial activity with 50% growth inhibition between 30 and 250 nM, on three Plasmodium falciparum strains differently sensitive to chloroquine. By affinity chromatography using one of these compounds, a 52-kDa prot...
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| Veröffentlicht in: | FEBS letters 2000-11, Vol.484 (3), p.246-252 |
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| creator | Florent, I Mouray, E Dali Ali, F Drobecq, H Girault, S Schrével, J Sergheraert, C Grellier, P Florenta, I |
| description | A series of 10 1,4-bis(3-aminopropyl)piperazine compounds was found to display antiplasmodial activity with 50% growth inhibition between 30 and 250 nM, on three Plasmodium falciparum strains differently sensitive to chloroquine. By affinity chromatography using one of these compounds, a 52-kDa protein was isolated from P. falciparum, microsequenced and cloned. It corresponded to a single copy gene encoding a 453 amino acid protein displaying the typical features of protein disulfide isomerases, a thiol metabolizing enzyme belonging to the thiol: disulfide oxidoreductase superfamily, which was not previously described in malarial species. |
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By affinity chromatography using one of these compounds, a 52-kDa protein was isolated from P. falciparum, microsequenced and cloned. 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Mouray, E ; Dali Ali, F ; Drobecq, H ; Girault, S ; Schrével, J ; Sergheraert, C ; Grellier, P ; Florenta, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p547-76ce8a5e366102390170e8c860cbe5ec4f503d1e574587ff240f717e4ce87ca83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antiprotozoal Agents</topic><topic>Base Sequence</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, Gel</topic><topic>Cloning, Molecular</topic><topic>Colombia</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Plasmodium falciparum - enzymology</topic><topic>Plasmodium falciparum - genetics</topic><topic>Plasmodium falciparum - isolation & purification</topic><topic>Protein Disulfide-Isomerases - genetics</topic><topic>Protein Disulfide-Isomerases - isolation & purification</topic><topic>Protein Disulfide-Isomerases - metabolism</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tanzania</topic><topic>Thailand</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Florent, I</creatorcontrib><creatorcontrib>Mouray, E</creatorcontrib><creatorcontrib>Dali Ali, F</creatorcontrib><creatorcontrib>Drobecq, H</creatorcontrib><creatorcontrib>Girault, S</creatorcontrib><creatorcontrib>Schrével, J</creatorcontrib><creatorcontrib>Sergheraert, C</creatorcontrib><creatorcontrib>Grellier, P</creatorcontrib><creatorcontrib>Florenta, I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Florent, I</au><au>Mouray, E</au><au>Dali Ali, F</au><au>Drobecq, H</au><au>Girault, S</au><au>Schrével, J</au><au>Sergheraert, C</au><au>Grellier, P</au><au>Florenta, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of Plasmodium falciparum protein disulfide isomerase homologue by affinity purification using the antiplasmodial inhibitor 1,4-bis[3-[N-(cyclohexyl methyl)amino]propyl]piperazine</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-11-10</date><risdate>2000</risdate><volume>484</volume><issue>3</issue><spage>246</spage><epage>252</epage><pages>246-252</pages><issn>0014-5793</issn><abstract>A series of 10 1,4-bis(3-aminopropyl)piperazine compounds was found to display antiplasmodial activity with 50% growth inhibition between 30 and 250 nM, on three Plasmodium falciparum strains differently sensitive to chloroquine. By affinity chromatography using one of these compounds, a 52-kDa protein was isolated from P. falciparum, microsequenced and cloned. It corresponded to a single copy gene encoding a 453 amino acid protein displaying the typical features of protein disulfide isomerases, a thiol metabolizing enzyme belonging to the thiol: disulfide oxidoreductase superfamily, which was not previously described in malarial species.</abstract><cop>England</cop><pmid>11078887</pmid><tpages>7</tpages></addata></record> |
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| ispartof | FEBS letters, 2000-11, Vol.484 (3), p.246-252 |
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| language | eng |
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| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antiprotozoal Agents Base Sequence Chromatography, Affinity Chromatography, Gel Cloning, Molecular Colombia Humans Molecular Sequence Data Molecular Weight Plasmodium falciparum - enzymology Plasmodium falciparum - genetics Plasmodium falciparum - isolation & purification Protein Disulfide-Isomerases - genetics Protein Disulfide-Isomerases - isolation & purification Protein Disulfide-Isomerases - metabolism Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Alignment Sequence Homology, Amino Acid Tanzania Thailand |
| title | Cloning of Plasmodium falciparum protein disulfide isomerase homologue by affinity purification using the antiplasmodial inhibitor 1,4-bis[3-[N-(cyclohexyl methyl)amino]propyl]piperazine |
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