Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology
Abstract With readily applicable hybridization assays, mainly based on rRNA-targeted nucleic acid probes, and direct, cultivation-independent sequence retrieval, microbiologists can for the first time determine the true composition of microbial communities. Phylogenetic identification and exact spat...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology reviews 2000-12, Vol.24 (5), p.555-565 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 565 |
|---|---|
| container_issue | 5 |
| container_start_page | 555 |
| container_title | FEMS microbiology reviews |
| container_volume | 24 |
| creator | Amann, Rudolf Ludwig, Wolfgang |
| description | Abstract
With readily applicable hybridization assays, mainly based on rRNA-targeted nucleic acid probes, and direct, cultivation-independent sequence retrieval, microbiologists can for the first time determine the true composition of microbial communities. Phylogenetic identification and exact spatiotemporal quantification of microorganisms will in the future become prerequisites for high quality studies in microbial ecology just as good taxonomy and solid quantification have always been for macroecology. This review is intended to give a short history of the development of rRNA-targeted nucleic acid probes and probe technologies, as well as of their application in microbial ecology. The current state of the art is described, and we will try to look into the future. Over the last decade, rRNA-targeted probes have become a handy tool for microbial ecologists. In order to speed up the transformation of microbial ecology from a mostly descriptive to a hypothesis-driven, experimental science more intense use must be made of the taxonomic precision and quantitativeness of rRNA-targeted probes. |
| doi_str_mv | 10.1111/j.1574-6976.2000.tb00557.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72413810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6976.2000.tb00557.x</oup_id><sourcerecordid>72413810</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5255-c0b627e964a338c2f60dde7dde305a65caaab292a5443b0f48fbdb979ee7d1463</originalsourceid><addsrcrecordid>eNqVkV1LHDEUhoNU3K3tX5DBgncz5uskM70oiLha_Cgseh2STEayzGy2kxl0_32z7lJBWsRASEie9805eRE6JrggaZwuCgKS56KSoqAY42IwGAPI4nkPTf9efUJTTESZC85hgj7HuEgoVAAHaEIIlpLwaoqu596EGDrdZvO7s3zQ_aMbXJ0tR9s6bzNtfZ2t-mBczJrQZ3EYa5_2fpl13qZzn5TOhjY8rr-g_Ua30X3drYfoYXZxf36V3_y6_Hl-dpNboAC5xUZQ6SrBNWOlpY3Ade1kmgyDFmC11oZWVAPnzOCGl42pTSUrlyDCBTtEJ1vfVNfv0cVBdT5a17Z66cIYlaScsJLgd0EipSAlqRL47Q24CGO_TE0oyhgThOIXu-9bKvUdY-8atep9p_u1IlhtklELtfl-tfl-tUlG7ZJRz0l8tHtiNJ2rX6W7KBLwYws8-datP2CtZrdzAEgGsDUI4-o_8vxflf0BEAKtHA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2333612010</pqid></control><display><type>article</type><title>Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology</title><source>Oxford Journals Open Access Collection</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Amann, Rudolf ; Ludwig, Wolfgang</creator><creatorcontrib>Amann, Rudolf ; Ludwig, Wolfgang</creatorcontrib><description>Abstract
With readily applicable hybridization assays, mainly based on rRNA-targeted nucleic acid probes, and direct, cultivation-independent sequence retrieval, microbiologists can for the first time determine the true composition of microbial communities. Phylogenetic identification and exact spatiotemporal quantification of microorganisms will in the future become prerequisites for high quality studies in microbial ecology just as good taxonomy and solid quantification have always been for macroecology. This review is intended to give a short history of the development of rRNA-targeted nucleic acid probes and probe technologies, as well as of their application in microbial ecology. The current state of the art is described, and we will try to look into the future. Over the last decade, rRNA-targeted probes have become a handy tool for microbial ecologists. In order to speed up the transformation of microbial ecology from a mostly descriptive to a hypothesis-driven, experimental science more intense use must be made of the taxonomic precision and quantitativeness of rRNA-targeted probes.</description><identifier>ISSN: 0168-6445</identifier><identifier>ISSN: 1574-6976</identifier><identifier>EISSN: 1574-6976</identifier><identifier>DOI: 10.1111/j.1574-6976.2000.tb00557.x</identifier><identifier>PMID: 11077149</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteria - classification ; Bacteria - genetics ; Bacteria - growth & development ; Bacterial Typing Techniques ; Bacteriological Techniques ; Community composition ; Cultivation ; DNA probes ; Ecological monitoring ; Ecology ; Ecosystem ; FISH ; History, 20th Century ; Hybridization ; Microbial activity ; Microbial ecology ; Microorganisms ; Molecular Probe Techniques ; Nucleic acid probe ; Nucleic acids ; Oligonucleotide Probes ; Phylogeny ; Probes ; RNA probes ; RNA, Bacterial - genetics ; RNA, Ribosomal - genetics ; rRNA ; Taxonomy</subject><ispartof>FEMS microbiology reviews, 2000-12, Vol.24 (5), p.555-565</ispartof><rights>2000 Federation of European Microbiological Societies. 2000</rights><rights>2000 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5255-c0b627e964a338c2f60dde7dde305a65caaab292a5443b0f48fbdb979ee7d1463</citedby><cites>FETCH-LOGICAL-c5255-c0b627e964a338c2f60dde7dde305a65caaab292a5443b0f48fbdb979ee7d1463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6976.2000.tb00557.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6976.2000.tb00557.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11077149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amann, Rudolf</creatorcontrib><creatorcontrib>Ludwig, Wolfgang</creatorcontrib><title>Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology</title><title>FEMS microbiology reviews</title><addtitle>FEMS Microbiol Rev</addtitle><description>Abstract
With readily applicable hybridization assays, mainly based on rRNA-targeted nucleic acid probes, and direct, cultivation-independent sequence retrieval, microbiologists can for the first time determine the true composition of microbial communities. Phylogenetic identification and exact spatiotemporal quantification of microorganisms will in the future become prerequisites for high quality studies in microbial ecology just as good taxonomy and solid quantification have always been for macroecology. This review is intended to give a short history of the development of rRNA-targeted nucleic acid probes and probe technologies, as well as of their application in microbial ecology. The current state of the art is described, and we will try to look into the future. Over the last decade, rRNA-targeted probes have become a handy tool for microbial ecologists. In order to speed up the transformation of microbial ecology from a mostly descriptive to a hypothesis-driven, experimental science more intense use must be made of the taxonomic precision and quantitativeness of rRNA-targeted probes.</description><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriological Techniques</subject><subject>Community composition</subject><subject>Cultivation</subject><subject>DNA probes</subject><subject>Ecological monitoring</subject><subject>Ecology</subject><subject>Ecosystem</subject><subject>FISH</subject><subject>History, 20th Century</subject><subject>Hybridization</subject><subject>Microbial activity</subject><subject>Microbial ecology</subject><subject>Microorganisms</subject><subject>Molecular Probe Techniques</subject><subject>Nucleic acid probe</subject><subject>Nucleic acids</subject><subject>Oligonucleotide Probes</subject><subject>Phylogeny</subject><subject>Probes</subject><subject>RNA probes</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal - genetics</subject><subject>rRNA</subject><subject>Taxonomy</subject><issn>0168-6445</issn><issn>1574-6976</issn><issn>1574-6976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkV1LHDEUhoNU3K3tX5DBgncz5uskM70oiLha_Cgseh2STEayzGy2kxl0_32z7lJBWsRASEie9805eRE6JrggaZwuCgKS56KSoqAY42IwGAPI4nkPTf9efUJTTESZC85hgj7HuEgoVAAHaEIIlpLwaoqu596EGDrdZvO7s3zQ_aMbXJ0tR9s6bzNtfZ2t-mBczJrQZ3EYa5_2fpl13qZzn5TOhjY8rr-g_Ua30X3drYfoYXZxf36V3_y6_Hl-dpNboAC5xUZQ6SrBNWOlpY3Ade1kmgyDFmC11oZWVAPnzOCGl42pTSUrlyDCBTtEJ1vfVNfv0cVBdT5a17Z66cIYlaScsJLgd0EipSAlqRL47Q24CGO_TE0oyhgThOIXu-9bKvUdY-8atep9p_u1IlhtklELtfl-tfl-tUlG7ZJRz0l8tHtiNJ2rX6W7KBLwYws8-datP2CtZrdzAEgGsDUI4-o_8vxflf0BEAKtHA</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Amann, Rudolf</creator><creator>Ludwig, Wolfgang</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20001201</creationdate><title>Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology</title><author>Amann, Rudolf ; Ludwig, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5255-c0b627e964a338c2f60dde7dde305a65caaab292a5443b0f48fbdb979ee7d1463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - growth & development</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriological Techniques</topic><topic>Community composition</topic><topic>Cultivation</topic><topic>DNA probes</topic><topic>Ecological monitoring</topic><topic>Ecology</topic><topic>Ecosystem</topic><topic>FISH</topic><topic>History, 20th Century</topic><topic>Hybridization</topic><topic>Microbial activity</topic><topic>Microbial ecology</topic><topic>Microorganisms</topic><topic>Molecular Probe Techniques</topic><topic>Nucleic acid probe</topic><topic>Nucleic acids</topic><topic>Oligonucleotide Probes</topic><topic>Phylogeny</topic><topic>Probes</topic><topic>RNA probes</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal - genetics</topic><topic>rRNA</topic><topic>Taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amann, Rudolf</creatorcontrib><creatorcontrib>Ludwig, Wolfgang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology reviews</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amann, Rudolf</au><au>Ludwig, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology</atitle><jtitle>FEMS microbiology reviews</jtitle><addtitle>FEMS Microbiol Rev</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>24</volume><issue>5</issue><spage>555</spage><epage>565</epage><pages>555-565</pages><issn>0168-6445</issn><issn>1574-6976</issn><eissn>1574-6976</eissn><abstract>Abstract
With readily applicable hybridization assays, mainly based on rRNA-targeted nucleic acid probes, and direct, cultivation-independent sequence retrieval, microbiologists can for the first time determine the true composition of microbial communities. Phylogenetic identification and exact spatiotemporal quantification of microorganisms will in the future become prerequisites for high quality studies in microbial ecology just as good taxonomy and solid quantification have always been for macroecology. This review is intended to give a short history of the development of rRNA-targeted nucleic acid probes and probe technologies, as well as of their application in microbial ecology. The current state of the art is described, and we will try to look into the future. Over the last decade, rRNA-targeted probes have become a handy tool for microbial ecologists. In order to speed up the transformation of microbial ecology from a mostly descriptive to a hypothesis-driven, experimental science more intense use must be made of the taxonomic precision and quantitativeness of rRNA-targeted probes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11077149</pmid><doi>10.1111/j.1574-6976.2000.tb00557.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-6445 |
| ispartof | FEMS microbiology reviews, 2000-12, Vol.24 (5), p.555-565 |
| issn | 0168-6445 1574-6976 1574-6976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72413810 |
| source | Oxford Journals Open Access Collection; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Bacteria - classification Bacteria - genetics Bacteria - growth & development Bacterial Typing Techniques Bacteriological Techniques Community composition Cultivation DNA probes Ecological monitoring Ecology Ecosystem FISH History, 20th Century Hybridization Microbial activity Microbial ecology Microorganisms Molecular Probe Techniques Nucleic acid probe Nucleic acids Oligonucleotide Probes Phylogeny Probes RNA probes RNA, Bacterial - genetics RNA, Ribosomal - genetics rRNA Taxonomy |
| title | Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T05%3A52%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ribosomal%20RNA-targeted%20nucleic%20acid%20probes%20for%20studies%20in%20microbial%20ecology&rft.jtitle=FEMS%20microbiology%20reviews&rft.au=Amann,%20Rudolf&rft.date=2000-12-01&rft.volume=24&rft.issue=5&rft.spage=555&rft.epage=565&rft.pages=555-565&rft.issn=0168-6445&rft.eissn=1574-6976&rft_id=info:doi/10.1111/j.1574-6976.2000.tb00557.x&rft_dat=%3Cproquest_cross%3E72413810%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2333612010&rft_id=info:pmid/11077149&rft_oup_id=10.1111/j.1574-6976.2000.tb00557.x&rfr_iscdi=true |