Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples

Highly sensitive assay systems are necessary for large-scale virological screenings. We evaluated the use of tyramide signal amplification (TSA) for biotinylated probe in dot-blot hybridization assay to detect B19 DNA in serum samples. The probe was constructed by PCR and directly labeled with bioti...

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Veröffentlicht in:	Clinica chimica acta 2000-12, Vol.302 (1), p.79-87
Hauptverfasser:	Zerbini, Marialuisa, Cricca, Monica, Gentilomi, Giovanna, Venturoli, Simona, Gallinella, Giorgio, Musiani, Monica
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotin Biotinylated probe Biotinylation DNA Probes DNA, Viral - blood Dot-blot hybridization assay Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Humans Microbiology Nucleic Acid Hybridization Parvoviridae Infections - diagnosis Parvovirus B19, Human - genetics Parvovirus B19, Human - isolation & purification Polymerase Chain Reaction Sensitivity and Specificity Techniques used in virology Tyramide signal amplification Virology
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