Delivery and pathway in MCF7 cells of DNA vectorized by cationic liposomes derived from cholesterol
We have investigated the delivery and the pathway in tumoral MCF7 cells of DNA carried by liposomes prepared from (trimethyl aminoethane carbamoyl cholesterol iodide (TMAE-Chol), a cholesterol-based cationic lipid with a quaternary ammonium on the polar head. The structure of DNA-liposome complexes...
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| Veröffentlicht in: | Antisense & nucleic acid drug development 2000-10, Vol.10 (5), p.369-380 |
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| container_title | Antisense & nucleic acid drug development |
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| creator | Cao, A Briane, D Coudert, R Vassy, J Lievre, N Olsman, E Tamboise, E Salzmann, J L Rigaut, J P Taillandier, E |
| description | We have investigated the delivery and the pathway in tumoral MCF7 cells of DNA carried by liposomes prepared from (trimethyl aminoethane carbamoyl cholesterol iodide (TMAE-Chol), a cholesterol-based cationic lipid with a quaternary ammonium on the polar head. The structure of DNA-liposome complexes depends on the length of DNA and on the lipid-DNA charge ratio X. Spherical beads constitute fine structures of the observed complexes even when they appear as aggregates. For oligonucleotide transfer, dissociation from liposomes after transfection, penetration of the oligonucleotides into nuclei, and a long resident time were observed. For plasmid transfer, a correlation between the variation in the transfection level and the ultrastructure of complexes was demonstrated. The results showed a cellular route of lipid/plasmid complexes from the beginning by endocytosis, entrapped into endosomes, released by the latter until entry in the perinuclear area, and then penetration of plasmids inside the nuclei resulting in the observed expression of the beta-galactosidase gene. |
| doi_str_mv | 10.1089/oli.1.2000.10.369 |
| format | Article |
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The structure of DNA-liposome complexes depends on the length of DNA and on the lipid-DNA charge ratio X. Spherical beads constitute fine structures of the observed complexes even when they appear as aggregates. For oligonucleotide transfer, dissociation from liposomes after transfection, penetration of the oligonucleotides into nuclei, and a long resident time were observed. For plasmid transfer, a correlation between the variation in the transfection level and the ultrastructure of complexes was demonstrated. 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The results showed a cellular route of lipid/plasmid complexes from the beginning by endocytosis, entrapped into endosomes, released by the latter until entry in the perinuclear area, and then penetration of plasmids inside the nuclei resulting in the observed expression of the beta-galactosidase gene.</description><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Biological Transport</subject><subject>Cations - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cholesterol - metabolism</subject><subject>DNA - administration & dosage</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>DNA - ultrastructure</subject><subject>Endocytosis</subject><subject>Endosomes - metabolism</subject><subject>Endosomes - ultrastructure</subject><subject>Fluorescein</subject><subject>Genetic Vectors - administration & dosage</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>Genetic Vectors - ultrastructure</subject><subject>Humans</subject><subject>Liposomes - chemistry</subject><subject>Liposomes - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron</subject><subject>Oligodeoxyribonucleotides - administration & dosage</subject><subject>Oligodeoxyribonucleotides - genetics</subject><subject>Oligodeoxyribonucleotides - metabolism</subject><subject>Transfection - methods</subject><subject>Tumor Cells, Cultured</subject><issn>1087-2906</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD9PwzAQxT2AaCl8ABbkiS3Bf5I4HquWAlKBBebIdc6qkVMHOy0Kn55ErcTIdLq7997pfgjdUJJSUsp772xKU0bI2Ke8kGdoOixEwiQpJugyxk9CKCtzfoEmlBIhc1FMkV6CswcIPVa7Greq236rHtsdflmsBNbgXMTe4OXrHB9Adz7YH6jxpsdaddbvrMbOtj76BiKuIQxRNTbBN1hvvYPYQfDuCp0b5SJcn-oMfawe3hdPyfrt8XkxXyeas7JLDDfE1IoVOq-10lAaKpnUGWMiq3OZ83LDuKGcSU6ZBshoaaQQCkqaQakVn6G7Y24b_Nd-OF41No4vqB34fawEy0jBOPlXSEWRCT6gmiF6FOrgYwxgqjbYRoW-oqQasVcD9opWI_ZxNGAfPLen8P2mgfrPcWLOfwFFZIDk</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Cao, A</creator><creator>Briane, D</creator><creator>Coudert, R</creator><creator>Vassy, J</creator><creator>Lievre, N</creator><creator>Olsman, E</creator><creator>Tamboise, E</creator><creator>Salzmann, J L</creator><creator>Rigaut, J P</creator><creator>Taillandier, E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Delivery and pathway in MCF7 cells of DNA vectorized by cationic liposomes derived from cholesterol</title><author>Cao, A ; 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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological Transport Cations - metabolism Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cholesterol - metabolism DNA - administration & dosage DNA - genetics DNA - metabolism DNA - ultrastructure Endocytosis Endosomes - metabolism Endosomes - ultrastructure Fluorescein Genetic Vectors - administration & dosage Genetic Vectors - genetics Genetic Vectors - metabolism Genetic Vectors - ultrastructure Humans Liposomes - chemistry Liposomes - metabolism Microscopy, Confocal Microscopy, Electron Oligodeoxyribonucleotides - administration & dosage Oligodeoxyribonucleotides - genetics Oligodeoxyribonucleotides - metabolism Transfection - methods Tumor Cells, Cultured |
| title | Delivery and pathway in MCF7 cells of DNA vectorized by cationic liposomes derived from cholesterol |
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