Protein Splicing-Based Reconstitution of Split Green Fluorescent Protein for Monitoring Protein−Protein Interactions in Bacteria:  Improved Sensitivity and Reduced Screening Time

In this research, an improved detection system is described that allows an easy in vivo screening and selection of functional interactions between two interacting proteins in bacteria. We earlier reported a new concept for detecting protein−protein interactions based on reconstitution of split-enhan...

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Veröffentlicht in:Analytical chemistry (Washington) 2001-12, Vol.73 (24), p.5866-5874
Hauptverfasser: Ozawa, Takeaki, Takeuchi, Mizue, Kaihara, Asami, Sato, Moritoshi, Umezawa, Yoshio
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Sprache:eng
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