Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration
We previously reported a novel glycosylphosphatidylinositol (GPI)‐anchored glycoprotein (tentatively designated GPI‐80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno‐ and scanning electron microscopy, the distri...
Gespeichert in:
| Veröffentlicht in: | Journal of leukocyte biology 2000-11, Vol.68 (5), p.650-654 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 654 |
|---|---|
| container_issue | 5 |
| container_start_page | 650 |
| container_title | Journal of leukocyte biology |
| container_volume | 68 |
| creator | Nakamura‐Sato, Yukiko Sasaki, Katsunori Watanabe, Hiroshi Araki, Yoshihiko Sendo, Fujiro |
| description | We previously reported a novel glycosylphosphatidylinositol (GPI)‐anchored glycoprotein (tentatively designated GPI‐80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno‐ and scanning electron microscopy, the distribution of GPI‐80 on neutrophil surfaces. GPI‐80 was diffusely distributed on the surface of resting neutrophils and on the peripheral areas of adherent cells after stimulation with N‐formyl‐methionyl‐leucyl‐phenylalanine. After longer stimulation (60 min), GPI‐80 decreased in number and was again diffusely distributed on the surfaces of round neutrophils. Few GPI‐80 were detected on the ventral surfaces of adherent neutrophils. Clusters of GPI‐80 were detected on the forward surfaces of neutrophils transmigrating through pores of nitrocellulose membranes. These results may give a morphological background of possible role of GPI‐80 for neutrophil extravasation. |
| doi_str_mv | 10.1189/jlb.68.5.650 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72400212</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17732687</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4280-b8667e5206b3525438830fca6dd1551dacf8efa340f0a8c09c4d137dc640f3ae3</originalsourceid><addsrcrecordid>eNqFkc2O0zAURi0EYsrAjjXyBlak2HHiuEuoYBhUCRawtm7t6yYjJy52QtS34JFxSflZwcqSfe75rPsR8pSzNedq8-rO79dSreu1rNk9suIboQohG3GfrFhT8aKuGLsij1K6Y4yJUrKH5Ipz1gjOxIp83_opjRi74UDDQMcWqQtxhmhpmqIDg4kGR_vuEGE8QwNOYwzHtvM_H4AO4Rt6evPptoDBtCGipccYRuzONhhpDyca8TB5GPGvaQq2xYiDQQqD_RUQhsfkgQOf8MnlvCZf3r39vH1f7D7e3G5f7wpTlYoVeyVlg3XJ5F7UZV0JpQRzBqS1vK65BeMUOhAVcwyUYRtTWS4aa2S-EYDimrxYvPmzXydMo-67ZNB7GDBMSTdl3lvJy_-CvGnyVlWTwZcLaGJIKaLTx9j1EE-aM32uSueqtFS61rmqjD-7eKd9j_YPfOkmA3wB5s7j6Z8y_WH3hi3S58tM2x3auYuoUw_e54hSz_P8O_wH0CmuiA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17732687</pqid></control><display><type>article</type><title>Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><source>EZB Electronic Journals Library</source><source>Oxford Journals</source><creator>Nakamura‐Sato, Yukiko ; Sasaki, Katsunori ; Watanabe, Hiroshi ; Araki, Yoshihiko ; Sendo, Fujiro</creator><creatorcontrib>Nakamura‐Sato, Yukiko ; Sasaki, Katsunori ; Watanabe, Hiroshi ; Araki, Yoshihiko ; Sendo, Fujiro</creatorcontrib><description>We previously reported a novel glycosylphosphatidylinositol (GPI)‐anchored glycoprotein (tentatively designated GPI‐80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno‐ and scanning electron microscopy, the distribution of GPI‐80 on neutrophil surfaces. GPI‐80 was diffusely distributed on the surface of resting neutrophils and on the peripheral areas of adherent cells after stimulation with N‐formyl‐methionyl‐leucyl‐phenylalanine. After longer stimulation (60 min), GPI‐80 decreased in number and was again diffusely distributed on the surfaces of round neutrophils. Few GPI‐80 were detected on the ventral surfaces of adherent neutrophils. Clusters of GPI‐80 were detected on the forward surfaces of neutrophils transmigrating through pores of nitrocellulose membranes. These results may give a morphological background of possible role of GPI‐80 for neutrophil extravasation.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.68.5.650</identifier><identifier>PMID: 11073103</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>Amidohydrolases ; Cell Adhesion - physiology ; Cell Adhesion Molecules - metabolism ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell Movement - physiology ; electron microscopy ; glycosylphosphatidylinositol-anchored proteins ; Glycosylphosphatidylinositols - metabolism ; GPI-80 protein ; GPI-Linked Proteins ; Humans ; Hydrolases ; integrin ; localization ; Microscopy, Electron, Scanning ; Microscopy, Immunoelectron ; N-Formylmethionine Leucyl-Phenylalanine - pharmacology ; Neutrophils - cytology ; Neutrophils - metabolism ; Neutrophils - ultrastructure ; Stimulation, Chemical</subject><ispartof>Journal of leukocyte biology, 2000-11, Vol.68 (5), p.650-654</ispartof><rights>2000 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4280-b8667e5206b3525438830fca6dd1551dacf8efa340f0a8c09c4d137dc640f3ae3</citedby><cites>FETCH-LOGICAL-c4280-b8667e5206b3525438830fca6dd1551dacf8efa340f0a8c09c4d137dc640f3ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.68.5.650$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.68.5.650$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11073103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakamura‐Sato, Yukiko</creatorcontrib><creatorcontrib>Sasaki, Katsunori</creatorcontrib><creatorcontrib>Watanabe, Hiroshi</creatorcontrib><creatorcontrib>Araki, Yoshihiko</creatorcontrib><creatorcontrib>Sendo, Fujiro</creatorcontrib><title>Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>We previously reported a novel glycosylphosphatidylinositol (GPI)‐anchored glycoprotein (tentatively designated GPI‐80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno‐ and scanning electron microscopy, the distribution of GPI‐80 on neutrophil surfaces. GPI‐80 was diffusely distributed on the surface of resting neutrophils and on the peripheral areas of adherent cells after stimulation with N‐formyl‐methionyl‐leucyl‐phenylalanine. After longer stimulation (60 min), GPI‐80 decreased in number and was again diffusely distributed on the surfaces of round neutrophils. Few GPI‐80 were detected on the ventral surfaces of adherent neutrophils. Clusters of GPI‐80 were detected on the forward surfaces of neutrophils transmigrating through pores of nitrocellulose membranes. These results may give a morphological background of possible role of GPI‐80 for neutrophil extravasation.</description><subject>Amidohydrolases</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Movement - physiology</subject><subject>electron microscopy</subject><subject>glycosylphosphatidylinositol-anchored proteins</subject><subject>Glycosylphosphatidylinositols - metabolism</subject><subject>GPI-80 protein</subject><subject>GPI-Linked Proteins</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>integrin</subject><subject>localization</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Immunoelectron</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Neutrophils - cytology</subject><subject>Neutrophils - metabolism</subject><subject>Neutrophils - ultrastructure</subject><subject>Stimulation, Chemical</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAURi0EYsrAjjXyBlak2HHiuEuoYBhUCRawtm7t6yYjJy52QtS34JFxSflZwcqSfe75rPsR8pSzNedq8-rO79dSreu1rNk9suIboQohG3GfrFhT8aKuGLsij1K6Y4yJUrKH5Ipz1gjOxIp83_opjRi74UDDQMcWqQtxhmhpmqIDg4kGR_vuEGE8QwNOYwzHtvM_H4AO4Rt6evPptoDBtCGipccYRuzONhhpDyca8TB5GPGvaQq2xYiDQQqD_RUQhsfkgQOf8MnlvCZf3r39vH1f7D7e3G5f7wpTlYoVeyVlg3XJ5F7UZV0JpQRzBqS1vK65BeMUOhAVcwyUYRtTWS4aa2S-EYDimrxYvPmzXydMo-67ZNB7GDBMSTdl3lvJy_-CvGnyVlWTwZcLaGJIKaLTx9j1EE-aM32uSueqtFS61rmqjD-7eKd9j_YPfOkmA3wB5s7j6Z8y_WH3hi3S58tM2x3auYuoUw_e54hSz_P8O_wH0CmuiA</recordid><startdate>200011</startdate><enddate>200011</enddate><creator>Nakamura‐Sato, Yukiko</creator><creator>Sasaki, Katsunori</creator><creator>Watanabe, Hiroshi</creator><creator>Araki, Yoshihiko</creator><creator>Sendo, Fujiro</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200011</creationdate><title>Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration</title><author>Nakamura‐Sato, Yukiko ; Sasaki, Katsunori ; Watanabe, Hiroshi ; Araki, Yoshihiko ; Sendo, Fujiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4280-b8667e5206b3525438830fca6dd1551dacf8efa340f0a8c09c4d137dc640f3ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amidohydrolases</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Movement - physiology</topic><topic>electron microscopy</topic><topic>glycosylphosphatidylinositol-anchored proteins</topic><topic>Glycosylphosphatidylinositols - metabolism</topic><topic>GPI-80 protein</topic><topic>GPI-Linked Proteins</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>integrin</topic><topic>localization</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Immunoelectron</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Neutrophils - cytology</topic><topic>Neutrophils - metabolism</topic><topic>Neutrophils - ultrastructure</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura‐Sato, Yukiko</creatorcontrib><creatorcontrib>Sasaki, Katsunori</creatorcontrib><creatorcontrib>Watanabe, Hiroshi</creatorcontrib><creatorcontrib>Araki, Yoshihiko</creatorcontrib><creatorcontrib>Sendo, Fujiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura‐Sato, Yukiko</au><au>Sasaki, Katsunori</au><au>Watanabe, Hiroshi</au><au>Araki, Yoshihiko</au><au>Sendo, Fujiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2000-11</date><risdate>2000</risdate><volume>68</volume><issue>5</issue><spage>650</spage><epage>654</epage><pages>650-654</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>We previously reported a novel glycosylphosphatidylinositol (GPI)‐anchored glycoprotein (tentatively designated GPI‐80) on human leukocytes that may be involved in the regulation of neutrophil adherence and migration. In this study, we examined by immuno‐ and scanning electron microscopy, the distribution of GPI‐80 on neutrophil surfaces. GPI‐80 was diffusely distributed on the surface of resting neutrophils and on the peripheral areas of adherent cells after stimulation with N‐formyl‐methionyl‐leucyl‐phenylalanine. After longer stimulation (60 min), GPI‐80 decreased in number and was again diffusely distributed on the surfaces of round neutrophils. Few GPI‐80 were detected on the ventral surfaces of adherent neutrophils. Clusters of GPI‐80 were detected on the forward surfaces of neutrophils transmigrating through pores of nitrocellulose membranes. These results may give a morphological background of possible role of GPI‐80 for neutrophil extravasation.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>11073103</pmid><doi>10.1189/jlb.68.5.650</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0741-5400 |
| ispartof | Journal of leukocyte biology, 2000-11, Vol.68 (5), p.650-654 |
| issn | 0741-5400 1938-3673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72400212 |
| source | MEDLINE; Wiley Online Library All Journals; EZB Electronic Journals Library; Oxford Journals |
| subjects | Amidohydrolases Cell Adhesion - physiology Cell Adhesion Molecules - metabolism Cell Membrane - metabolism Cell Membrane - ultrastructure Cell Movement - physiology electron microscopy glycosylphosphatidylinositol-anchored proteins Glycosylphosphatidylinositols - metabolism GPI-80 protein GPI-Linked Proteins Humans Hydrolases integrin localization Microscopy, Electron, Scanning Microscopy, Immunoelectron N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutrophils - cytology Neutrophils - metabolism Neutrophils - ultrastructure Stimulation, Chemical |
| title | Clustering on the forward surfaces of migrating neutrophils of a novel GPI-anchored protein that may regulate neutrophil adherence and migration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T16%3A49%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clustering%20on%20the%20forward%20surfaces%20of%20migrating%20neutrophils%20of%20a%20novel%20GPI-anchored%20protein%20that%20may%20regulate%20neutrophil%20adherence%20and%20migration&rft.jtitle=Journal%20of%20leukocyte%20biology&rft.au=Nakamura%E2%80%90Sato,%20Yukiko&rft.date=2000-11&rft.volume=68&rft.issue=5&rft.spage=650&rft.epage=654&rft.pages=650-654&rft.issn=0741-5400&rft.eissn=1938-3673&rft_id=info:doi/10.1189/jlb.68.5.650&rft_dat=%3Cproquest_cross%3E17732687%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17732687&rft_id=info:pmid/11073103&rfr_iscdi=true |