Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?

The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpre...

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Veröffentlicht in:	Journal of industrial microbiology & biotechnology 2001-11, Vol.27 (5), p.307-313
Hauptverfasser:	Thormann, K, Dürre, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetone - metabolism Alcohol Dehydrogenase - genetics Aldehyde Oxidoreductases - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding sites Biotechnology Butanols - metabolism Cloning, Molecular Clostridium - genetics Clostridium acetobutylicum Coenzyme A-Transferases Deoxyribonucleic acid DNA DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism E coli Enzymes Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes Genes, Bacterial Genetic engineering Microbiology Multienzyme Complexes - genetics Operon - genetics Orf5 gene Plasmids Proteins Repressor Proteins - genetics Repressor Proteins - metabolism Solvents Solvents - metabolism Studies
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