Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?
The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpre...
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| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 2001-11, Vol.27 (5), p.307-313 |
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| creator | Thormann, K Dürre, P |
| description | The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpression of Orf5 in C. acetobutylicum led to a change in the organism's pattern of glycosylated exoproteins. The Orf5 protein was localized in the cell membrane fraction and to a small extent in the supernatant medium. Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation. |
| doi_str_mv | 10.1038/sj.jim.7000144 |
| format | Article |
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The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpression of Orf5 in C. acetobutylicum led to a change in the organism's pattern of glycosylated exoproteins. The Orf5 protein was localized in the cell membrane fraction and to a small extent in the supernatant medium. Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1038/sj.jim.7000144</identifier><identifier>PMID: 11781806</identifier><language>eng</language><publisher>Germany: Oxford University Press</publisher><subject>Acetone - metabolism ; Alcohol Dehydrogenase - genetics ; Aldehyde Oxidoreductases - genetics ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Binding sites ; Biotechnology ; Butanols - metabolism ; Cloning, Molecular ; Clostridium - genetics ; Clostridium acetobutylicum ; Coenzyme A-Transferases ; Deoxyribonucleic acid ; DNA ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; E coli ; Enzymes ; Escherichia coli - genetics ; Escherichia coli Proteins ; Gene Expression Regulation, Bacterial ; Genes ; Genes, Bacterial ; Genetic engineering ; Microbiology ; Multienzyme Complexes - genetics ; Operon - genetics ; Orf5 gene ; Plasmids ; Proteins ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Solvents ; Solvents - metabolism ; Studies</subject><ispartof>Journal of industrial microbiology & biotechnology, 2001-11, Vol.27 (5), p.307-313</ispartof><rights>Society for Industrial Microbiology 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-cc6ef8f3d3908a6dc89e7404ff16b4587b638c6aa7c4c75336e1fcb923a6a81a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11781806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thormann, K</creatorcontrib><creatorcontrib>Dürre, P</creatorcontrib><title>Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><description>The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpression of Orf5 in C. acetobutylicum led to a change in the organism's pattern of glycosylated exoproteins. The Orf5 protein was localized in the cell membrane fraction and to a small extent in the supernatant medium. Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation.</description><subject>Acetone - metabolism</subject><subject>Alcohol Dehydrogenase - genetics</subject><subject>Aldehyde Oxidoreductases - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Binding sites</subject><subject>Biotechnology</subject><subject>Butanols - metabolism</subject><subject>Cloning, Molecular</subject><subject>Clostridium - genetics</subject><subject>Clostridium acetobutylicum</subject><subject>Coenzyme A-Transferases</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Proteins</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Microbiology</subject><subject>Multienzyme Complexes - genetics</subject><subject>Operon - genetics</subject><subject>Orf5 gene</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Solvents</subject><subject>Solvents - metabolism</subject><subject>Studies</subject><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtLxDAURoMozji6dSnFhbvOJL151Y3I4AsGBnzgsqRpgi1tU5N2Mf_eDlMQ3Li6l8v5vsU9CF0SvCQY5CpUy6pslgJjTCg9QnNCBY8ZA3Y87sBFzCiwGToLoRoZJkRyimaECEkk5nP0ufWWrd5c_Xobqaj3qg3al11fulbVkTedNyE4Hzkb9V8mCq6OXGe8a_eXde1C78uiHJpIadO7fOh3damH5u4cnVhVB3MxzQX6eHx4Xz_Hm-3Ty_p-E2uQaR9rzY2VFgpIsVS80DI1gmJqLeE5ZVLkHKTmSglNtWAA3BCr8zQBxZUkChbo5tDbefc9mNBnTRm0qWvVGjeETCSQ8gTDvyCRQCFJ5Ahe_wErN_jxG2MZwykWCecjtDxA2rsQvLFZ58tG-V1GcLYXk4UqG8Vkk5gxcDW1Dnljil98MgE_OumKIg</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Thormann, K</creator><creator>Dürre, P</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?</title><author>Thormann, K ; Dürre, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-cc6ef8f3d3908a6dc89e7404ff16b4587b638c6aa7c4c75336e1fcb923a6a81a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acetone - 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genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Solvents</topic><topic>Solvents - metabolism</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thormann, K</creatorcontrib><creatorcontrib>Dürre, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of industrial microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thormann, K</au><au>Dürre, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>27</volume><issue>5</issue><spage>307</spage><epage>313</epage><pages>307-313</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpression of Orf5 in C. acetobutylicum led to a change in the organism's pattern of glycosylated exoproteins. The Orf5 protein was localized in the cell membrane fraction and to a small extent in the supernatant medium. Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation.</abstract><cop>Germany</cop><pub>Oxford University Press</pub><pmid>11781806</pmid><doi>10.1038/sj.jim.7000144</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of industrial microbiology & biotechnology, 2001-11, Vol.27 (5), p.307-313 |
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| subjects | Acetone - metabolism Alcohol Dehydrogenase - genetics Aldehyde Oxidoreductases - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding sites Biotechnology Butanols - metabolism Cloning, Molecular Clostridium - genetics Clostridium acetobutylicum Coenzyme A-Transferases Deoxyribonucleic acid DNA DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism E coli Enzymes Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes Genes, Bacterial Genetic engineering Microbiology Multienzyme Complexes - genetics Operon - genetics Orf5 gene Plasmids Proteins Repressor Proteins - genetics Repressor Proteins - metabolism Solvents Solvents - metabolism Studies |
| title | Orf5/SolR: a transcriptional repressor of the sol operon of Clostridium acetobutylicum? |
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