Transcriptional repression of type I procollagen genes during adipocyte differentiation
Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gen...
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| Veröffentlicht in: | Experimental & molecular medicine 2001-12, Vol.33 (4), p.269-275 |
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| creator | Yi, T Choi, H M Park, R W Sohn, K Y Kim, I S |
| description | Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both alpha1 and alpha2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery. |
| doi_str_mv | 10.1038/emm.2001.44 |
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Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both alpha1 and alpha2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.</description><identifier>ISSN: 1226-3613</identifier><identifier>ISSN: 2092-6413</identifier><identifier>EISSN: 2092-6413</identifier><identifier>DOI: 10.1038/emm.2001.44</identifier><identifier>PMID: 11795491</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>3T3 Cells ; Adipocytes - cytology ; Adipocytes - metabolism ; Animals ; Cell Differentiation - genetics ; Cell Line ; Collagen Type I - genetics ; Collagen Type I - metabolism ; Down-Regulation - genetics ; Gene Expression Regulation ; Genes, Reporter ; Kinetics ; Mice ; Mutation ; Procollagen - genetics ; Procollagen - metabolism ; Promoter Regions, Genetic ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; RNA, Messenger - metabolism ; Transcription, Genetic</subject><ispartof>Experimental & molecular medicine, 2001-12, Vol.33 (4), p.269-275</ispartof><rights>Copyright Nature Publishing Group Dec 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-551b6e69b9d8876f45cff6ab175ee5095d4d184bf8dd040ae644b4e7fe4d0dd13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11795491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, T</creatorcontrib><creatorcontrib>Choi, H M</creatorcontrib><creatorcontrib>Park, R W</creatorcontrib><creatorcontrib>Sohn, K Y</creatorcontrib><creatorcontrib>Kim, I S</creatorcontrib><title>Transcriptional repression of type I procollagen genes during adipocyte differentiation</title><title>Experimental & molecular medicine</title><addtitle>Exp Mol Med</addtitle><description>Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both alpha1 and alpha2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.</description><subject>3T3 Cells</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Animals</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Line</subject><subject>Collagen Type I - genetics</subject><subject>Collagen Type I - metabolism</subject><subject>Down-Regulation - genetics</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Kinetics</subject><subject>Mice</subject><subject>Mutation</subject><subject>Procollagen - genetics</subject><subject>Procollagen - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription, Genetic</subject><issn>1226-3613</issn><issn>2092-6413</issn><issn>2092-6413</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1r3DAQxUVpaDabnHIPgkApFG801ki2jmFp00Agl4QcjWyNgoO_KtmH_e8jk4VCDj0Mw8CP9x7zGLsEsQMhyxvq-10uBOwQv7BNLkyeaQT5lW0gz3UmNchTdhbjmxC5wgK_sVOAwig0sGEvT8EOsQntNLfjYDseaAoUYzr46Pl8mIjf8ymMzdh19pUGnoYid0toh1duXTuNzWEm7lrvKdAwt3ZVOmcn3naRLo57y55__3ra_8keHu_u97cPWSNLOWdKQa1Jm9q4siy0R9V4r20NhSJSwiiHDkqsfemcQGFJI9ZIhSd0wjmQW_b9QzdF_LtQnKu-jQ2lrAONS6yKXBoloEzgj_-CyUVLgyLXCb3-hL6NS0jPWSkQSoIRq_PPD6oJY4yBfDWFtrfhUIGo1mKqVEy1FlMhJvrqqLnUPbl_7LEJ-Q6hpYm5</recordid><startdate>20011231</startdate><enddate>20011231</enddate><creator>Yi, T</creator><creator>Choi, H M</creator><creator>Park, R W</creator><creator>Sohn, K Y</creator><creator>Kim, I S</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20011231</creationdate><title>Transcriptional repression of type I procollagen genes during adipocyte differentiation</title><author>Yi, T ; 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Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both alpha1 and alpha2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>11795491</pmid><doi>10.1038/emm.2001.44</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3 Cells Adipocytes - cytology Adipocytes - metabolism Animals Cell Differentiation - genetics Cell Line Collagen Type I - genetics Collagen Type I - metabolism Down-Regulation - genetics Gene Expression Regulation Genes, Reporter Kinetics Mice Mutation Procollagen - genetics Procollagen - metabolism Promoter Regions, Genetic Repressor Proteins - genetics Repressor Proteins - metabolism RNA, Messenger - metabolism Transcription, Genetic |
| title | Transcriptional repression of type I procollagen genes during adipocyte differentiation |
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