Functional Dissection of the Dimerization and Enzymatic Activities of Escherichia coli Nitrogen Regulator II and Their Regulation by the PII Protein

The dimeric two-component system transmitter protein NRII (NtrB) of Escherichia coli, product of glnL (ntrB), controls transcription of nitrogen-regulated genes by catalyzing the phosphorylation and dephosphorylation of the transcription factor NRI (NtrC). Previous studies showed that the PII signal...

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Veröffentlicht in:	Biochemistry (Easton) 2000-11, Vol.39 (44), p.13433-13449
Hauptverfasser:	Jiang, Peng, Atkinson, Mariette R, Srisawat, Chatchawan, Sun, Quan, Ninfa, Alexander J
Format:	Artikel
Sprache:	eng
Schlagworte:	ATP-Binding Cassette Transporters Bacterial Proteins - physiology Binding Sites - genetics Carrier Proteins - genetics Carrier Proteins - isolation & purification Dimerization Enzyme Activation - genetics Escherichia coli Escherichia coli - enzymology Escherichia coli - metabolism Escherichia coli Proteins Maltose-Binding Proteins Monosaccharide Transport Proteins Nitrogen regulator II NtrB protein NtrC protein Peptide Fragments - genetics Peptide Fragments - isolation & purification Phosphoprotein Phosphatases - antagonists & inhibitors Phosphoprotein Phosphatases - genetics Phosphoprotein Phosphatases - isolation & purification Phosphoprotein Phosphatases - metabolism Phosphorylation PII Nitrogen Regulatory Proteins PII protein Protein Conformation Protein Kinase Inhibitors Protein Kinases - genetics Protein Kinases - isolation & purification Protein Kinases - metabolism Protein Structure, Tertiary - genetics Sequence Deletion Signal Transduction - genetics Structure-Activity Relationship
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