A Region Upstream of the Human δ-Globin Gene Shows a Stage-Specific Interaction with Globin Promoters in Erythroid Cell Lines
ABSTRACT We previously showed that the 651-bp DNA fragment, located 3 kb upstream from the human |gd-globin gene (fragment F5), is able to inhibit adult, not fetal, globin promoter in mouse erythroleukemia cell lines (MEL) expressing adult globin genes. Here we show in transient analysis that fragme...
Gespeichert in:
| Veröffentlicht in: | Blood cells, molecules, & diseases molecules, & diseases, 2001-09, Vol.27 (5), p.874-881 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 881 |
|---|---|
| container_issue | 5 |
| container_start_page | 874 |
| container_title | Blood cells, molecules, & diseases |
| container_volume | 27 |
| creator | Vitale, Maria Calzolari, Roberta Di Marzo, Rosalba Acuto, Santina Maggio, Aurelio |
| description | ABSTRACT
We previously showed that the 651-bp DNA fragment, located 3 kb upstream from the human |gd-globin gene (fragment F5), is able to inhibit adult, not fetal, globin promoter in mouse erythroleukemia cell lines (MEL) expressing adult globin genes. Here we show in transient analysis that fragment F5 has a strong inhibitory effect on fetal |gg-globin promoter in human erythroleukemia cell lines (HEL) expressing fetal globin genes. Since the |gb-promoter constructs were poorly expressed in fetal cells, new plasmids containing an HPFH promoter (Gγ−175, T to C), which is strongly expressed in both fetal and adult cell lines, were made. Here we report that fragment F5 in HEL cells has a strong inhibitory effect on wild-type γ-promoter only; no effect was evident on γ−175-promoter in either MEL or HEL cell lines. Altogether these results show a stage-specific interaction between fragment F5 and globin promoters during development. We also report the presence of several bindings for erythroid GATA family factors by electrophoretic mobility shift assay, using nuclear extracts from erythroid cell lines. |
| doi_str_mv | 10.1006/bcmd.2001.0447 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72389899</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1079979601904474</els_id><sourcerecordid>72389899</sourcerecordid><originalsourceid>FETCH-LOGICAL-c369t-8288d157934342dbf6c8fa40f08f2a6bffbd051a1bae5f2b8798f4fcb8a2565e3</originalsourceid><addsrcrecordid>eNp1kMtOAyEUhonReN-6NKzcTYW5wtI0Wk2aaKxdE2AOLaYzVKAaNz6Vz-EzyaRNXLkgB3K-8-fwIXRByYgSUl8r3bWjnBA6ImXZ7KFjSnidpUP3h3vDM97w-gidhPBKEkY5O0RHlDas4BU5Rl83-BkW1vV4vg7Rg-ywMzguAd9vOtnjn-9ssnLK9ngCPeDZ0n0ELPEsygVkszVoa6zGD30EL3Uccj5sXOLdzJN3nUutgNPj1n_GpXe2xWNYrfDU9hDO0IGRqwDnu3qK5ne3L-P7bPo4eRjfTDNd1DxmLGespVXDi7Io81aZWjMjS2IIM7mslTGqJRWVVEmoTK5Yw5kpjVZM5lVdQXGKrra5a-_eNhCi6GzQaQ3Zg9sE0eQF44zzBI62oPYuBA9GrL3tpP8UlIjBuBiMi8G4GIyngctd8kZ10P7hO8UJYFsA0v_eLXgRtIVeQ2s96ChaZ__L_gVgDpFC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72389899</pqid></control><display><type>article</type><title>A Region Upstream of the Human δ-Globin Gene Shows a Stage-Specific Interaction with Globin Promoters in Erythroid Cell Lines</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Vitale, Maria ; Calzolari, Roberta ; Di Marzo, Rosalba ; Acuto, Santina ; Maggio, Aurelio</creator><creatorcontrib>Vitale, Maria ; Calzolari, Roberta ; Di Marzo, Rosalba ; Acuto, Santina ; Maggio, Aurelio</creatorcontrib><description>ABSTRACT
We previously showed that the 651-bp DNA fragment, located 3 kb upstream from the human |gd-globin gene (fragment F5), is able to inhibit adult, not fetal, globin promoter in mouse erythroleukemia cell lines (MEL) expressing adult globin genes. Here we show in transient analysis that fragment F5 has a strong inhibitory effect on fetal |gg-globin promoter in human erythroleukemia cell lines (HEL) expressing fetal globin genes. Since the |gb-promoter constructs were poorly expressed in fetal cells, new plasmids containing an HPFH promoter (Gγ−175, T to C), which is strongly expressed in both fetal and adult cell lines, were made. Here we report that fragment F5 in HEL cells has a strong inhibitory effect on wild-type γ-promoter only; no effect was evident on γ−175-promoter in either MEL or HEL cell lines. Altogether these results show a stage-specific interaction between fragment F5 and globin promoters during development. We also report the presence of several bindings for erythroid GATA family factors by electrophoretic mobility shift assay, using nuclear extracts from erythroid cell lines.</description><identifier>ISSN: 1079-9796</identifier><identifier>EISSN: 1096-0961</identifier><identifier>DOI: 10.1006/bcmd.2001.0447</identifier><identifier>PMID: 11783950</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Binding Sites ; DNA Footprinting ; DNA-Binding Proteins - metabolism ; Erythroid Precursor Cells - metabolism ; Fetal Proteins - genetics ; Gene Expression Regulation, Developmental - genetics ; Globins - genetics ; Humans ; Mice ; Promoter Regions, Genetic - genetics ; Transfection ; Tumor Cells, Cultured</subject><ispartof>Blood cells, molecules, & diseases, 2001-09, Vol.27 (5), p.874-881</ispartof><rights>2001 Elsevier Science (USA)</rights><rights>(c)2001 Elsevier Science.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-8288d157934342dbf6c8fa40f08f2a6bffbd051a1bae5f2b8798f4fcb8a2565e3</citedby><cites>FETCH-LOGICAL-c369t-8288d157934342dbf6c8fa40f08f2a6bffbd051a1bae5f2b8798f4fcb8a2565e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bcmd.2001.0447$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11783950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vitale, Maria</creatorcontrib><creatorcontrib>Calzolari, Roberta</creatorcontrib><creatorcontrib>Di Marzo, Rosalba</creatorcontrib><creatorcontrib>Acuto, Santina</creatorcontrib><creatorcontrib>Maggio, Aurelio</creatorcontrib><title>A Region Upstream of the Human δ-Globin Gene Shows a Stage-Specific Interaction with Globin Promoters in Erythroid Cell Lines</title><title>Blood cells, molecules, & diseases</title><addtitle>Blood Cells Mol Dis</addtitle><description>ABSTRACT
We previously showed that the 651-bp DNA fragment, located 3 kb upstream from the human |gd-globin gene (fragment F5), is able to inhibit adult, not fetal, globin promoter in mouse erythroleukemia cell lines (MEL) expressing adult globin genes. Here we show in transient analysis that fragment F5 has a strong inhibitory effect on fetal |gg-globin promoter in human erythroleukemia cell lines (HEL) expressing fetal globin genes. Since the |gb-promoter constructs were poorly expressed in fetal cells, new plasmids containing an HPFH promoter (Gγ−175, T to C), which is strongly expressed in both fetal and adult cell lines, were made. Here we report that fragment F5 in HEL cells has a strong inhibitory effect on wild-type γ-promoter only; no effect was evident on γ−175-promoter in either MEL or HEL cell lines. Altogether these results show a stage-specific interaction between fragment F5 and globin promoters during development. We also report the presence of several bindings for erythroid GATA family factors by electrophoretic mobility shift assay, using nuclear extracts from erythroid cell lines.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>DNA Footprinting</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Erythroid Precursor Cells - metabolism</subject><subject>Fetal Proteins - genetics</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Globins - genetics</subject><subject>Humans</subject><subject>Mice</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>1079-9796</issn><issn>1096-0961</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtOAyEUhonReN-6NKzcTYW5wtI0Wk2aaKxdE2AOLaYzVKAaNz6Vz-EzyaRNXLkgB3K-8-fwIXRByYgSUl8r3bWjnBA6ImXZ7KFjSnidpUP3h3vDM97w-gidhPBKEkY5O0RHlDas4BU5Rl83-BkW1vV4vg7Rg-ywMzguAd9vOtnjn-9ssnLK9ngCPeDZ0n0ELPEsygVkszVoa6zGD30EL3Uccj5sXOLdzJN3nUutgNPj1n_GpXe2xWNYrfDU9hDO0IGRqwDnu3qK5ne3L-P7bPo4eRjfTDNd1DxmLGespVXDi7Io81aZWjMjS2IIM7mslTGqJRWVVEmoTK5Yw5kpjVZM5lVdQXGKrra5a-_eNhCi6GzQaQ3Zg9sE0eQF44zzBI62oPYuBA9GrL3tpP8UlIjBuBiMi8G4GIyngctd8kZ10P7hO8UJYFsA0v_eLXgRtIVeQ2s96ChaZ__L_gVgDpFC</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Vitale, Maria</creator><creator>Calzolari, Roberta</creator><creator>Di Marzo, Rosalba</creator><creator>Acuto, Santina</creator><creator>Maggio, Aurelio</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010901</creationdate><title>A Region Upstream of the Human δ-Globin Gene Shows a Stage-Specific Interaction with Globin Promoters in Erythroid Cell Lines</title><author>Vitale, Maria ; Calzolari, Roberta ; Di Marzo, Rosalba ; Acuto, Santina ; Maggio, Aurelio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-8288d157934342dbf6c8fa40f08f2a6bffbd051a1bae5f2b8798f4fcb8a2565e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>DNA Footprinting</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Erythroid Precursor Cells - metabolism</topic><topic>Fetal Proteins - genetics</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Globins - genetics</topic><topic>Humans</topic><topic>Mice</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vitale, Maria</creatorcontrib><creatorcontrib>Calzolari, Roberta</creatorcontrib><creatorcontrib>Di Marzo, Rosalba</creatorcontrib><creatorcontrib>Acuto, Santina</creatorcontrib><creatorcontrib>Maggio, Aurelio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood cells, molecules, & diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vitale, Maria</au><au>Calzolari, Roberta</au><au>Di Marzo, Rosalba</au><au>Acuto, Santina</au><au>Maggio, Aurelio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Region Upstream of the Human δ-Globin Gene Shows a Stage-Specific Interaction with Globin Promoters in Erythroid Cell Lines</atitle><jtitle>Blood cells, molecules, & diseases</jtitle><addtitle>Blood Cells Mol Dis</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>27</volume><issue>5</issue><spage>874</spage><epage>881</epage><pages>874-881</pages><issn>1079-9796</issn><eissn>1096-0961</eissn><abstract>ABSTRACT
We previously showed that the 651-bp DNA fragment, located 3 kb upstream from the human |gd-globin gene (fragment F5), is able to inhibit adult, not fetal, globin promoter in mouse erythroleukemia cell lines (MEL) expressing adult globin genes. Here we show in transient analysis that fragment F5 has a strong inhibitory effect on fetal |gg-globin promoter in human erythroleukemia cell lines (HEL) expressing fetal globin genes. Since the |gb-promoter constructs were poorly expressed in fetal cells, new plasmids containing an HPFH promoter (Gγ−175, T to C), which is strongly expressed in both fetal and adult cell lines, were made. Here we report that fragment F5 in HEL cells has a strong inhibitory effect on wild-type γ-promoter only; no effect was evident on γ−175-promoter in either MEL or HEL cell lines. Altogether these results show a stage-specific interaction between fragment F5 and globin promoters during development. We also report the presence of several bindings for erythroid GATA family factors by electrophoretic mobility shift assay, using nuclear extracts from erythroid cell lines.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11783950</pmid><doi>10.1006/bcmd.2001.0447</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1079-9796 |
| ispartof | Blood cells, molecules, & diseases, 2001-09, Vol.27 (5), p.874-881 |
| issn | 1079-9796 1096-0961 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72389899 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Base Sequence Binding Sites DNA Footprinting DNA-Binding Proteins - metabolism Erythroid Precursor Cells - metabolism Fetal Proteins - genetics Gene Expression Regulation, Developmental - genetics Globins - genetics Humans Mice Promoter Regions, Genetic - genetics Transfection Tumor Cells, Cultured |
| title | A Region Upstream of the Human δ-Globin Gene Shows a Stage-Specific Interaction with Globin Promoters in Erythroid Cell Lines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T19%3A58%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Region%20Upstream%20of%20the%20Human%20%CE%B4-Globin%20Gene%20Shows%20a%20Stage-Specific%20Interaction%20with%20Globin%20Promoters%20in%20Erythroid%20Cell%20Lines&rft.jtitle=Blood%20cells,%20molecules,%20&%20diseases&rft.au=Vitale,%20Maria&rft.date=2001-09-01&rft.volume=27&rft.issue=5&rft.spage=874&rft.epage=881&rft.pages=874-881&rft.issn=1079-9796&rft.eissn=1096-0961&rft_id=info:doi/10.1006/bcmd.2001.0447&rft_dat=%3Cproquest_cross%3E72389899%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72389899&rft_id=info:pmid/11783950&rft_els_id=S1079979601904474&rfr_iscdi=true |