Allele-Specific Non-CpG Methylation of the Nf1 Gene during Early Mouse Development
Recent reports of cytosine methylation occurring at CpA and CpT dinucleotides in murine ES cells as well as in Drosophila have renewed interest in methylation at sites other than CpGs. Our examination of the murine neurofibromatosis type 1 gene by sodium bisulfite genomic sequencing has revealed non...
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| Veröffentlicht in: | Developmental biology 2001-12, Vol.240 (2), p.585-598 |
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| creator | Haines, Thomas R. Rodenhiser, David I. Ainsworth, Peter J. |
| description | Recent reports of cytosine methylation occurring at CpA and CpT dinucleotides in murine ES cells as well as in Drosophila have renewed interest in methylation at sites other than CpGs. Our examination of the murine neurofibromatosis type 1 gene by sodium bisulfite genomic sequencing has revealed non-CpG methylation primarily in the oocyte and the maternally derived allele of the 2-cell embryo, with markedly lower levels found in sperm. Non-CpG methylation was not found in later stages of embryo development or in adult tissue. Our results suggest that maternal-specific de novo non-CpG methylation has occurred sometime between ovulation and formation of the 2-cell embryo, while during the same period the paternally derived allele has undergone site-specific active demethylation. Our data demonstrate both stage and parent-of-origin specific changes in methylation patterns within the neurofibromatosis type 1 coding region-involving cytosines located at both CpG and non-CpG dinucleotides. |
| doi_str_mv | 10.1006/dbio.2001.0504 |
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Our examination of the murine neurofibromatosis type 1 gene by sodium bisulfite genomic sequencing has revealed non-CpG methylation primarily in the oocyte and the maternally derived allele of the 2-cell embryo, with markedly lower levels found in sperm. Non-CpG methylation was not found in later stages of embryo development or in adult tissue. Our results suggest that maternal-specific de novo non-CpG methylation has occurred sometime between ovulation and formation of the 2-cell embryo, while during the same period the paternally derived allele has undergone site-specific active demethylation. 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Our examination of the murine neurofibromatosis type 1 gene by sodium bisulfite genomic sequencing has revealed non-CpG methylation primarily in the oocyte and the maternally derived allele of the 2-cell embryo, with markedly lower levels found in sperm. Non-CpG methylation was not found in later stages of embryo development or in adult tissue. Our results suggest that maternal-specific de novo non-CpG methylation has occurred sometime between ovulation and formation of the 2-cell embryo, while during the same period the paternally derived allele has undergone site-specific active demethylation. Our data demonstrate both stage and parent-of-origin specific changes in methylation patterns within the neurofibromatosis type 1 coding region-involving cytosines located at both CpG and non-CpG dinucleotides.</description><subject>Adenosine Deaminase - genetics</subject><subject>allele-specific</subject><subject>Alleles</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cleavage Stage, Ovum - metabolism</subject><subject>CpG Islands</subject><subject>Crosses, Genetic</subject><subject>development</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA Methylation</subject><subject>Embryonic and Fetal Development - genetics</subject><subject>Female</subject><subject>Genes, Neurofibromatosis 1</subject><subject>Humans</subject><subject>Male</subject><subject>Methylation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Molecular Sequence Data</subject><subject>mouse</subject><subject>Muridae</subject><subject>neurofibromatosis</subject><subject>Nf1 gene</subject><subject>non-CpG</subject><subject>Oocytes - metabolism</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><subject>Spermatozoa - metabolism</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEURUVJaRy32y6LVtmN8zT6GHlpHNcJOCm0DWQnZjRPjYo8mkhjg_99x9jQVcjqweXcy-MQ8pXBjAGom7bxcVYCsBlIEB_IhMFcFlKJ5wsyGeOyYArUJbnK-S8AcK35J3LJWKUFaDkhPxchYMDiV4_WO2_pY-yKZb-mDzi8HEI9-NjR6OjwgvTRMbrGDmm7S777Q1d1Cgf6EHcZ6S3uMcR-i93wmXx0dcj45Xyn5On76vfyrtj8WN8vF5vCiqoaCsW1nVeVk6WrSsWs0E5yWzbCAoITyjYtCoA5bznjY6PUWpcSUcnGCVk5PiXXp90-xdcd5sFsfbYYQt3h-JOpSq7nXOl3QaY5aD3iUzI7gTbFnBM60ye_rdPBMDBH3eao2xx1m6PusfDtvLxrttj-x89-R0CfABxF7D0mk63HzmLrE9rBtNG_tf0PV8OMgw</recordid><startdate>20011215</startdate><enddate>20011215</enddate><creator>Haines, Thomas R.</creator><creator>Rodenhiser, David I.</creator><creator>Ainsworth, Peter J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20011215</creationdate><title>Allele-Specific Non-CpG Methylation of the Nf1 Gene during Early Mouse Development</title><author>Haines, Thomas R. ; Rodenhiser, David I. ; Ainsworth, Peter J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-638c977f52f7261c48f53c2b4c0e0f46cbde40093d313477288825ee65bf457f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenosine Deaminase - genetics</topic><topic>allele-specific</topic><topic>Alleles</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cleavage Stage, Ovum - metabolism</topic><topic>CpG Islands</topic><topic>Crosses, Genetic</topic><topic>development</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA Methylation</topic><topic>Embryonic and Fetal Development - genetics</topic><topic>Female</topic><topic>Genes, Neurofibromatosis 1</topic><topic>Humans</topic><topic>Male</topic><topic>Methylation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Molecular Sequence Data</topic><topic>mouse</topic><topic>Muridae</topic><topic>neurofibromatosis</topic><topic>Nf1 gene</topic><topic>non-CpG</topic><topic>Oocytes - metabolism</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Species Specificity</topic><topic>Spermatozoa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haines, Thomas R.</creatorcontrib><creatorcontrib>Rodenhiser, David I.</creatorcontrib><creatorcontrib>Ainsworth, Peter J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haines, Thomas R.</au><au>Rodenhiser, David I.</au><au>Ainsworth, Peter J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allele-Specific Non-CpG Methylation of the Nf1 Gene during Early Mouse Development</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2001-12-15</date><risdate>2001</risdate><volume>240</volume><issue>2</issue><spage>585</spage><epage>598</epage><pages>585-598</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Recent reports of cytosine methylation occurring at CpA and CpT dinucleotides in murine ES cells as well as in Drosophila have renewed interest in methylation at sites other than CpGs. Our examination of the murine neurofibromatosis type 1 gene by sodium bisulfite genomic sequencing has revealed non-CpG methylation primarily in the oocyte and the maternally derived allele of the 2-cell embryo, with markedly lower levels found in sperm. Non-CpG methylation was not found in later stages of embryo development or in adult tissue. Our results suggest that maternal-specific de novo non-CpG methylation has occurred sometime between ovulation and formation of the 2-cell embryo, while during the same period the paternally derived allele has undergone site-specific active demethylation. Our data demonstrate both stage and parent-of-origin specific changes in methylation patterns within the neurofibromatosis type 1 coding region-involving cytosines located at both CpG and non-CpG dinucleotides.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11784085</pmid><doi>10.1006/dbio.2001.0504</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Deaminase - genetics allele-specific Alleles Animals Base Sequence Cleavage Stage, Ovum - metabolism CpG Islands Crosses, Genetic development DNA - chemistry DNA - genetics DNA Methylation Embryonic and Fetal Development - genetics Female Genes, Neurofibromatosis 1 Humans Male Methylation Mice Mice, Inbred C57BL Mice, Inbred CBA Molecular Sequence Data mouse Muridae neurofibromatosis Nf1 gene non-CpG Oocytes - metabolism Sequence Homology, Nucleic Acid Species Specificity Spermatozoa - metabolism |
| title | Allele-Specific Non-CpG Methylation of the Nf1 Gene during Early Mouse Development |
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