Purified hematopoietic stem cells can differentiate into hepatocytes in vivo
The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH −/− mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreo...
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| Veröffentlicht in: | Nature medicine 2000-11, Vol.6 (11), p.1229-1234 |
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| creator | Lagasse, Eric Connors, Heather Al-Dhalimy, Muhsen Reitsma, Michael Dohse, Monika Osborne, Linda Wang, Xin Finegold, Milton Weissman, Irving L. Grompe, Markus |
| description | The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH
−/−
mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells. |
| doi_str_mv | 10.1038/81326 |
| format | Article |
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−/−
mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.</description><identifier>ISSN: 1078-8956</identifier><identifier>EISSN: 1546-170X</identifier><identifier>DOI: 10.1038/81326</identifier><identifier>PMID: 11062533</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Animal models ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Bone marrow ; Bone Marrow Cells - cytology ; Cancer Research ; Cell Differentiation ; Cell Separation - methods ; Cell Transplantation ; Cellular therapy ; Enzymes ; Genetic aspects ; Health aspects ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hepatocytes - cytology ; Hydrolases - deficiency ; Infectious Diseases ; Injection ; Liver ; Liver - pathology ; Liver cells ; Liver diseases ; Liver Regeneration ; Medicine ; Metabolic Diseases ; Mice ; Mice, Inbred C57BL ; Mice, Inbred Strains ; Mice, Knockout ; Molecular Medicine ; Neurosciences ; Stem cell transplantation ; Stem cells ; Tyrosinemias - genetics ; Tyrosinemias - pathology ; Tyrosinemias - therapy ; Whole-Body Irradiation</subject><ispartof>Nature medicine, 2000-11, Vol.6 (11), p.1229-1234</ispartof><rights>Nature America Inc. 2000</rights><rights>COPYRIGHT 2000 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Nov 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-faf70300f7c49d37555a78b4766bee35993592bd87196c0226f2cf4775f284423</citedby><cites>FETCH-LOGICAL-c566t-faf70300f7c49d37555a78b4766bee35993592bd87196c0226f2cf4775f284423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/81326$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/81326$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11062533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lagasse, Eric</creatorcontrib><creatorcontrib>Connors, Heather</creatorcontrib><creatorcontrib>Al-Dhalimy, Muhsen</creatorcontrib><creatorcontrib>Reitsma, Michael</creatorcontrib><creatorcontrib>Dohse, Monika</creatorcontrib><creatorcontrib>Osborne, Linda</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Finegold, Milton</creatorcontrib><creatorcontrib>Weissman, Irving L.</creatorcontrib><creatorcontrib>Grompe, Markus</creatorcontrib><title>Purified hematopoietic stem cells can differentiate into hepatocytes in vivo</title><title>Nature medicine</title><addtitle>Nat Med</addtitle><addtitle>Nat Med</addtitle><description>The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH
−/−
mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cancer Research</subject><subject>Cell Differentiation</subject><subject>Cell Separation - methods</subject><subject>Cell Transplantation</subject><subject>Cellular therapy</subject><subject>Enzymes</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hepatocytes - cytology</subject><subject>Hydrolases - 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Academic</collection><jtitle>Nature medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lagasse, Eric</au><au>Connors, Heather</au><au>Al-Dhalimy, Muhsen</au><au>Reitsma, Michael</au><au>Dohse, Monika</au><au>Osborne, Linda</au><au>Wang, Xin</au><au>Finegold, Milton</au><au>Weissman, Irving L.</au><au>Grompe, Markus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purified hematopoietic stem cells can differentiate into hepatocytes in vivo</atitle><jtitle>Nature medicine</jtitle><stitle>Nat Med</stitle><addtitle>Nat Med</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>6</volume><issue>11</issue><spage>1229</spage><epage>1234</epage><pages>1229-1234</pages><issn>1078-8956</issn><eissn>1546-170X</eissn><abstract>The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH
−/−
mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>11062533</pmid><doi>10.1038/81326</doi><tpages>6</tpages></addata></record> |
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| issn | 1078-8956 1546-170X |
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| source | MEDLINE; Nature; SpringerLink Journals - AutoHoldings |
| subjects | Animal models Animals Biomedical and Life Sciences Biomedicine Bone marrow Bone Marrow Cells - cytology Cancer Research Cell Differentiation Cell Separation - methods Cell Transplantation Cellular therapy Enzymes Genetic aspects Health aspects Hematopoietic stem cells Hematopoietic Stem Cells - cytology Hepatocytes - cytology Hydrolases - deficiency Infectious Diseases Injection Liver Liver - pathology Liver cells Liver diseases Liver Regeneration Medicine Metabolic Diseases Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Knockout Molecular Medicine Neurosciences Stem cell transplantation Stem cells Tyrosinemias - genetics Tyrosinemias - pathology Tyrosinemias - therapy Whole-Body Irradiation |
| title | Purified hematopoietic stem cells can differentiate into hepatocytes in vivo |
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