Lumican and decorin are differentially expressed in human breast carcinoma
Previous studies have shown that lumican is expressed and increased in the stroma of breast tumours. Lumican expression has now been examined relative to other members of the small leucine‐rich proteoglycan gene family in normal and neoplastic breast tissues, to begin to determine its role in breast...
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| Veröffentlicht in: | The Journal of pathology 2000-11, Vol.192 (3), p.313-320 |
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| container_title | The Journal of pathology |
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| creator | Leygue, Etienne Snell, Linda Dotzlaw, Helmut Troup, Sandra Hiller-Hitchcock, Tamara Murphy, Leigh C. Roughley, Peter J. Watson, Peter H. |
| description | Previous studies have shown that lumican is expressed and increased in the stroma of breast tumours. Lumican expression has now been examined relative to other members of the small leucine‐rich proteoglycan gene family in normal and neoplastic breast tissues, to begin to determine its role in breast tumour progression. Western blot study showed that lumican protein is highly abundant relative to decorin, while biglycan and fibromodulin are only detected occasionally in breast tissues (n=15 cases). Further analysis of lumican and decorin expression performed in matched normal and tumour tissues by in situ hybridization showed that both mRNAs were expressed by similar fibroblast‐like cells adjacent to epithelium. However, lumican mRNA expression was significantly increased in tumours (n=34, p |
| doi_str_mv | 10.1002/1096-9896(200011)192:3<313::AID-PATH694>3.0.CO;2-B |
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Lumican expression has now been examined relative to other members of the small leucine‐rich proteoglycan gene family in normal and neoplastic breast tissues, to begin to determine its role in breast tumour progression. Western blot study showed that lumican protein is highly abundant relative to decorin, while biglycan and fibromodulin are only detected occasionally in breast tissues (n=15 cases). Further analysis of lumican and decorin expression performed in matched normal and tumour tissues by in situ hybridization showed that both mRNAs were expressed by similar fibroblast‐like cells adjacent to epithelium. However, lumican mRNA expression was significantly increased in tumours (n=34, p<0.0001), while decorin mRNA was decreased (p=0.0002) in neoplastic relative to adjacent normal stroma. This was accompanied by a significant increase in lumican protein (n=12, p=0.0122), but not decorin. Further evidence of altered lumican expression in breast cancer was manifested by discordance between lumican mRNA and protein localization in some regions of tumours but not in adjacent morphologically normal tissues. It is concluded that lumican is the most abundant of these proteoglycans in breast tumours and that lumican and decorin are inversely regulated in association with breast tumourigenesis. Copyright © 2000 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/1096-9896(200011)192:3<313::AID-PATH694>3.0.CO;2-B</identifier><identifier>PMID: 11054714</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Biomarkers, Tumor ; Blotting, Western ; breast cancer ; Breast Neoplasms - metabolism ; Case-Control Studies ; decorin ; Disease Progression ; Electrophoresis, Polyacrylamide Gel ; Female ; Gene Expression ; Gynecology. Andrology. Obstetrics ; Humans ; In Situ Hybridization ; lumican ; Mammary gland diseases ; Medical sciences ; Middle Aged ; Proteoglycans - genetics ; Proteoglycans - metabolism ; RNA, Messenger - analysis ; small leucine-rich proteoglycan ; Tumors ; tumour progression</subject><ispartof>The Journal of pathology, 2000-11, Vol.192 (3), p.313-320</ispartof><rights>Copyright © 2000 John Wiley & Sons, Ltd.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2000 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4934-8456a2ead7e46f4add09ddfefc3ccc7a305f6dc7e1994be8c33b50d35074e9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1096-9896%28200011%29192%3A3%3C313%3A%3AAID-PATH694%3E3.0.CO%3B2-B$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1096-9896%28200011%29192%3A3%3C313%3A%3AAID-PATH694%3E3.0.CO%3B2-B$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=792932$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11054714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leygue, Etienne</creatorcontrib><creatorcontrib>Snell, Linda</creatorcontrib><creatorcontrib>Dotzlaw, Helmut</creatorcontrib><creatorcontrib>Troup, Sandra</creatorcontrib><creatorcontrib>Hiller-Hitchcock, Tamara</creatorcontrib><creatorcontrib>Murphy, Leigh C.</creatorcontrib><creatorcontrib>Roughley, Peter J.</creatorcontrib><creatorcontrib>Watson, Peter H.</creatorcontrib><title>Lumican and decorin are differentially expressed in human breast carcinoma</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>Previous studies have shown that lumican is expressed and increased in the stroma of breast tumours. Lumican expression has now been examined relative to other members of the small leucine‐rich proteoglycan gene family in normal and neoplastic breast tissues, to begin to determine its role in breast tumour progression. Western blot study showed that lumican protein is highly abundant relative to decorin, while biglycan and fibromodulin are only detected occasionally in breast tissues (n=15 cases). Further analysis of lumican and decorin expression performed in matched normal and tumour tissues by in situ hybridization showed that both mRNAs were expressed by similar fibroblast‐like cells adjacent to epithelium. However, lumican mRNA expression was significantly increased in tumours (n=34, p<0.0001), while decorin mRNA was decreased (p=0.0002) in neoplastic relative to adjacent normal stroma. This was accompanied by a significant increase in lumican protein (n=12, p=0.0122), but not decorin. Further evidence of altered lumican expression in breast cancer was manifested by discordance between lumican mRNA and protein localization in some regions of tumours but not in adjacent morphologically normal tissues. It is concluded that lumican is the most abundant of these proteoglycans in breast tumours and that lumican and decorin are inversely regulated in association with breast tumourigenesis. Copyright © 2000 John Wiley & Sons, Ltd.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor</subject><subject>Blotting, Western</subject><subject>breast cancer</subject><subject>Breast Neoplasms - metabolism</subject><subject>Case-Control Studies</subject><subject>decorin</subject><subject>Disease Progression</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>lumican</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Proteoglycans - genetics</subject><subject>Proteoglycans - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>small leucine-rich proteoglycan</subject><subject>Tumors</subject><subject>tumour progression</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF2LEzEUQIMobnf1L8iAIO7D1GSSmTRVhG7V_aBrl6Xo4yWT3MHofNSkg9t_b8qM9cUXnxKSk5PLIWTO6JRRmr1hVBWpmqnidUYpZeycqWzO33HG5_PF9Yf0brG5KpR4z6d0uly_zdKLR2RyfPSYTKIkS7lg8oSchvA9SpTK86fkhDGaC8nEhNys-sYZ3Sa6tYlF03kX9x4T66oKPbY7p-t6n-DD1mMIaJN4_61v4ovSow67xGhvXNs1-hl5Uuk64PNxPSObTx83y6t0tb68Xi5WqRGKi3Qm8kJnqK1EUVRCW0uVtRVWhhtjpOY0rwprJDKlRIkzw3mZU8tzKgWqkp-RV4N267ufPYYdNC4YrGvdYtcHkBmXShSzCN4PoPFdCB4r2HrXaL8HRuEQGA6t4NAKhsAQAwOHGBggBoYxcDyisFxDBhdR-mL8vS8btH-VY9EIvBwBHYyuK69b48KRkypTPIvUl4H65Wrc_89g_57rz1EUp4PYhR0-HMXa_4BCcpnD18-XcHvP7_jq9gYE_w0WM7Eg</recordid><startdate>200011</startdate><enddate>200011</enddate><creator>Leygue, Etienne</creator><creator>Snell, Linda</creator><creator>Dotzlaw, Helmut</creator><creator>Troup, Sandra</creator><creator>Hiller-Hitchcock, Tamara</creator><creator>Murphy, Leigh C.</creator><creator>Roughley, Peter J.</creator><creator>Watson, Peter H.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200011</creationdate><title>Lumican and decorin are differentially expressed in human breast carcinoma</title><author>Leygue, Etienne ; Snell, Linda ; Dotzlaw, Helmut ; Troup, Sandra ; Hiller-Hitchcock, Tamara ; Murphy, Leigh C. ; Roughley, Peter J. ; Watson, Peter H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4934-8456a2ead7e46f4add09ddfefc3ccc7a305f6dc7e1994be8c33b50d35074e9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor</topic><topic>Blotting, Western</topic><topic>breast cancer</topic><topic>Breast Neoplasms - metabolism</topic><topic>Case-Control Studies</topic><topic>decorin</topic><topic>Disease Progression</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>lumican</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Proteoglycans - genetics</topic><topic>Proteoglycans - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>small leucine-rich proteoglycan</topic><topic>Tumors</topic><topic>tumour progression</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leygue, Etienne</creatorcontrib><creatorcontrib>Snell, Linda</creatorcontrib><creatorcontrib>Dotzlaw, Helmut</creatorcontrib><creatorcontrib>Troup, Sandra</creatorcontrib><creatorcontrib>Hiller-Hitchcock, Tamara</creatorcontrib><creatorcontrib>Murphy, Leigh C.</creatorcontrib><creatorcontrib>Roughley, Peter J.</creatorcontrib><creatorcontrib>Watson, Peter H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leygue, Etienne</au><au>Snell, Linda</au><au>Dotzlaw, Helmut</au><au>Troup, Sandra</au><au>Hiller-Hitchcock, Tamara</au><au>Murphy, Leigh C.</au><au>Roughley, Peter J.</au><au>Watson, Peter H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lumican and decorin are differentially expressed in human breast carcinoma</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>2000-11</date><risdate>2000</risdate><volume>192</volume><issue>3</issue><spage>313</spage><epage>320</epage><pages>313-320</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>Previous studies have shown that lumican is expressed and increased in the stroma of breast tumours. Lumican expression has now been examined relative to other members of the small leucine‐rich proteoglycan gene family in normal and neoplastic breast tissues, to begin to determine its role in breast tumour progression. Western blot study showed that lumican protein is highly abundant relative to decorin, while biglycan and fibromodulin are only detected occasionally in breast tissues (n=15 cases). Further analysis of lumican and decorin expression performed in matched normal and tumour tissues by in situ hybridization showed that both mRNAs were expressed by similar fibroblast‐like cells adjacent to epithelium. However, lumican mRNA expression was significantly increased in tumours (n=34, p<0.0001), while decorin mRNA was decreased (p=0.0002) in neoplastic relative to adjacent normal stroma. This was accompanied by a significant increase in lumican protein (n=12, p=0.0122), but not decorin. Further evidence of altered lumican expression in breast cancer was manifested by discordance between lumican mRNA and protein localization in some regions of tumours but not in adjacent morphologically normal tissues. It is concluded that lumican is the most abundant of these proteoglycans in breast tumours and that lumican and decorin are inversely regulated in association with breast tumourigenesis. Copyright © 2000 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>11054714</pmid><doi>10.1002/1096-9896(200011)192:3<313::AID-PATH694>3.0.CO;2-B</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Biological and medical sciences Biomarkers, Tumor Blotting, Western breast cancer Breast Neoplasms - metabolism Case-Control Studies decorin Disease Progression Electrophoresis, Polyacrylamide Gel Female Gene Expression Gynecology. Andrology. Obstetrics Humans In Situ Hybridization lumican Mammary gland diseases Medical sciences Middle Aged Proteoglycans - genetics Proteoglycans - metabolism RNA, Messenger - analysis small leucine-rich proteoglycan Tumors tumour progression |
| title | Lumican and decorin are differentially expressed in human breast carcinoma |
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