Low Leukocyte Contamination without Filtration by Preparation of Platelet Concentrates in Cylindrical Bags with the Buffy-Coat Method
Background and Objectives: The buffy‐coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration. Materials and Methods: BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optim...
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| Veröffentlicht in: | Vox sanguinis 2000-09, Vol.79 (2), p.87-93 |
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| creator | Kostelijk, E.H. Loos, J.A. Veldman, H.A. de Korte, D. |
| description | Background and Objectives:
The buffy‐coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration. Materials and Methods: BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined. Results: With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 ± 0.19 versus 3.0 ± 1.71 × 106 leukocytes per unit; 85‐ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 ± 16.4 versus 61 ± 15 × 109 platelets per PC). Furthermore, when PCs were prepared from 100‐ml BCs in cylindrical bags, a leukocyte contamination of 0.2 ± 0.11 × 106 and a platelet content of 6 ± 13.5 × 109 per PC were obtained. Conclusion: The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko‐depletion without affecting platelet recovery. |
| doi_str_mv | 10.1046/j.1423-0410.2000.7920087.x |
| format | Article |
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The buffy‐coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration. Materials and Methods: BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined. Results: With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 ± 0.19 versus 3.0 ± 1.71 × 106 leukocytes per unit; 85‐ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 ± 16.4 versus 61 ± 15 × 109 platelets per PC). Furthermore, when PCs were prepared from 100‐ml BCs in cylindrical bags, a leukocyte contamination of 0.2 ± 0.11 × 106 and a platelet content of 6 ± 13.5 × 109 per PC were obtained. Conclusion: The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko‐depletion without affecting platelet recovery.</description><identifier>ISSN: 0042-9007</identifier><identifier>EISSN: 1423-0410</identifier><identifier>DOI: 10.1046/j.1423-0410.2000.7920087.x</identifier><identifier>PMID: 11054046</identifier><identifier>CODEN: VOSAAD</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Cell Separation - instrumentation ; Centrifugation - methods ; Equipment and Supplies - standards ; Filtration ; Hematocrit ; Humans ; Leukocyte Count ; Leukocytes ; Medical sciences ; Platelet Count ; Plateletpheresis - methods ; Plateletpheresis - standards ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Vox sanguinis, 2000-09, Vol.79 (2), p.87-93</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 S. Karger AG, Basel</rights><rights>Copyright S. Karger AG Sep 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4047-7109fb44316ff973bb3f2357306b9b98bb6e16fd93ce288e5ba6135c5a71645e3</citedby><cites>FETCH-LOGICAL-c4047-7109fb44316ff973bb3f2357306b9b98bb6e16fd93ce288e5ba6135c5a71645e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1423-0410.2000.7920087.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1423-0410.2000.7920087.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1533954$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11054046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kostelijk, E.H.</creatorcontrib><creatorcontrib>Loos, J.A.</creatorcontrib><creatorcontrib>Veldman, H.A.</creatorcontrib><creatorcontrib>de Korte, D.</creatorcontrib><title>Low Leukocyte Contamination without Filtration by Preparation of Platelet Concentrates in Cylindrical Bags with the Buffy-Coat Method</title><title>Vox sanguinis</title><addtitle>Vox Sang</addtitle><description>Background and Objectives:
The buffy‐coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration. Materials and Methods: BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined. Results: With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 ± 0.19 versus 3.0 ± 1.71 × 106 leukocytes per unit; 85‐ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 ± 16.4 versus 61 ± 15 × 109 platelets per PC). Furthermore, when PCs were prepared from 100‐ml BCs in cylindrical bags, a leukocyte contamination of 0.2 ± 0.11 × 106 and a platelet content of 6 ± 13.5 × 109 per PC were obtained. Conclusion: The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko‐depletion without affecting platelet recovery.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Cell Separation - instrumentation</subject><subject>Centrifugation - methods</subject><subject>Equipment and Supplies - standards</subject><subject>Filtration</subject><subject>Hematocrit</subject><subject>Humans</subject><subject>Leukocyte Count</subject><subject>Leukocytes</subject><subject>Medical sciences</subject><subject>Platelet Count</subject><subject>Plateletpheresis - methods</subject><subject>Plateletpheresis - standards</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0042-9007</issn><issn>1423-0410</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc9u1DAQxi0EokvhFZBVIW5ZHP-JE240YgvSlvYABfViOdkJ9TYbL7aj3TwA741DolbihmRpNDO_-WbkD6GzlCxTwrN322XKKUsIjwVKCFnKIoZcLo9P0OKh9RQtCOE0KQiRJ-iF99uI5jQXz9FJmhLBo9QC_V7bA15Df2_rIQAubRf0znQ6GNvhgwl3tg94ZdrgplI14GsHez2ntsHXrQ7QQhhna-hGEDw2HS6H1nQbZ2rd4nP90_-Vw-EO8HnfNENSWh3wJcQVm5foWaNbD6_meIq-rT5-LT8l66uLz-WHdVLHa2UiU1I0FecszZqmkKyqWEOZkIxkVVEVeVVlEFubgtVA8xxEpbOUiVpomWZcADtFbyfdvbO_evBB7YyvoW11B7b3SlImeZHRCJ79A25t77p4m6KU8ZyzPIvQ-wmqnfXeQaP2zuy0G1RK1OiU2qrRDjXaoUan1OyUOsbh1_OGvtrB5nF0tiYCb2ZA-_iFjdNdbfwjJxgrBI_YasIOpoXhPy5QN1c_4ou5GgtRKJmEjA9wfBDS7l5lkkmhvn-5ULekFOXtDVWX7A8jPsDs</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>Kostelijk, E.H.</creator><creator>Loos, J.A.</creator><creator>Veldman, H.A.</creator><creator>de Korte, D.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>S. Karger AG</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200009</creationdate><title>Low Leukocyte Contamination without Filtration by Preparation of Platelet Concentrates in Cylindrical Bags with the Buffy-Coat Method</title><author>Kostelijk, E.H. ; Loos, J.A. ; Veldman, H.A. ; de Korte, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4047-7109fb44316ff973bb3f2357306b9b98bb6e16fd93ce288e5ba6135c5a71645e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Cell Separation - instrumentation</topic><topic>Centrifugation - methods</topic><topic>Equipment and Supplies - standards</topic><topic>Filtration</topic><topic>Hematocrit</topic><topic>Humans</topic><topic>Leukocyte Count</topic><topic>Leukocytes</topic><topic>Medical sciences</topic><topic>Platelet Count</topic><topic>Plateletpheresis - methods</topic><topic>Plateletpheresis - standards</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kostelijk, E.H.</creatorcontrib><creatorcontrib>Loos, J.A.</creatorcontrib><creatorcontrib>Veldman, H.A.</creatorcontrib><creatorcontrib>de Korte, D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Vox sanguinis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kostelijk, E.H.</au><au>Loos, J.A.</au><au>Veldman, H.A.</au><au>de Korte, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low Leukocyte Contamination without Filtration by Preparation of Platelet Concentrates in Cylindrical Bags with the Buffy-Coat Method</atitle><jtitle>Vox sanguinis</jtitle><addtitle>Vox Sang</addtitle><date>2000-09</date><risdate>2000</risdate><volume>79</volume><issue>2</issue><spage>87</spage><epage>93</epage><pages>87-93</pages><issn>0042-9007</issn><eissn>1423-0410</eissn><coden>VOSAAD</coden><abstract>Background and Objectives:
The buffy‐coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration. Materials and Methods: BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined. Results: With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 ± 0.19 versus 3.0 ± 1.71 × 106 leukocytes per unit; 85‐ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 ± 16.4 versus 61 ± 15 × 109 platelets per PC). Furthermore, when PCs were prepared from 100‐ml BCs in cylindrical bags, a leukocyte contamination of 0.2 ± 0.11 × 106 and a platelet content of 6 ± 13.5 × 109 per PC were obtained. Conclusion: The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko‐depletion without affecting platelet recovery.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11054046</pmid><doi>10.1046/j.1423-0410.2000.7920087.x</doi><tpages>7</tpages></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; Karger Journals; MEDLINE |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Cell Separation - instrumentation Centrifugation - methods Equipment and Supplies - standards Filtration Hematocrit Humans Leukocyte Count Leukocytes Medical sciences Platelet Count Plateletpheresis - methods Plateletpheresis - standards Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | Low Leukocyte Contamination without Filtration by Preparation of Platelet Concentrates in Cylindrical Bags with the Buffy-Coat Method |
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