Effect of extraction pH on metal speciation in plant root extracts
The species patterns of nutrient and trace metals (K, Ca, Mg, Mn, Fe, Zn) obtained by extraction of plant roots have been determined as a function of extraction pH in the range 4-9. The extractable metal concentrations were subdivided into low-molecular-weight (10 kDa) metal species by TXRF analysis...
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| Veröffentlicht in: | Fresenius' journal of analytical chemistry 2001-12, Vol.371 (7), p.921-926 |
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| container_title | Fresenius' journal of analytical chemistry |
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| creator | WEBER, G MESSERSCHMIDT, J VON BOHLEN, A ALT, F |
| description | The species patterns of nutrient and trace metals (K, Ca, Mg, Mn, Fe, Zn) obtained by extraction of plant roots have been determined as a function of extraction pH in the range 4-9. The extractable metal concentrations were subdivided into low-molecular-weight (10 kDa) metal species by TXRF analysis. Except for pH 9, the low-molecular-weight fraction is predominant. This fraction was further separated by HPLC with AAS detection. It was demonstrated that the nature of the metal species changes dramatically when the extraction pH is changed, especially in the range 9-7. Information about the chemical nature of chromatographically separated metal species is obtained by use of different electrochemical detectors (amperometric detection at a glassy carbon or copper electrode and pulsed amperometric detection) and diode-array UV detection. |
| doi_str_mv | 10.1007/s002160101056 |
| format | Article |
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The extractable metal concentrations were subdivided into low-molecular-weight (<10 kDa) and high-molecular-weight (>10 kDa) metal species by TXRF analysis. Except for pH 9, the low-molecular-weight fraction is predominant. This fraction was further separated by HPLC with AAS detection. It was demonstrated that the nature of the metal species changes dramatically when the extraction pH is changed, especially in the range 9-7. Information about the chemical nature of chromatographically separated metal species is obtained by use of different electrochemical detectors (amperometric detection at a glassy carbon or copper electrode and pulsed amperometric detection) and diode-array UV detection.</description><identifier>ISSN: 0937-0633</identifier><identifier>EISSN: 1432-1130</identifier><identifier>DOI: 10.1007/s002160101056</identifier><identifier>PMID: 11769801</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject><![CDATA[Biological and medical sciences ; Calcium - analysis ; Calcium - chemistry ; Calcium - isolation & purification ; Chemical constitution ; Fundamental and applied biological sciences. 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The extractable metal concentrations were subdivided into low-molecular-weight (<10 kDa) and high-molecular-weight (>10 kDa) metal species by TXRF analysis. Except for pH 9, the low-molecular-weight fraction is predominant. This fraction was further separated by HPLC with AAS detection. It was demonstrated that the nature of the metal species changes dramatically when the extraction pH is changed, especially in the range 9-7. Information about the chemical nature of chromatographically separated metal species is obtained by use of different electrochemical detectors (amperometric detection at a glassy carbon or copper electrode and pulsed amperometric detection) and diode-array UV detection.</description><subject>Biological and medical sciences</subject><subject>Calcium - analysis</subject><subject>Calcium - chemistry</subject><subject>Calcium - isolation & purification</subject><subject>Chemical constitution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Iron - analysis</subject><subject>Iron - chemistry</subject><subject>Iron - isolation & purification</subject><subject>Manganese - analysis</subject><subject>Manganese - chemistry</subject><subject>Manganese - isolation & purification</subject><subject>Metals - analysis</subject><subject>Metals - chemistry</subject><subject>Metals - isolation & purification</subject><subject>Organometallic Compounds - analysis</subject><subject>Organometallic Compounds - chemistry</subject><subject>Organometallic Compounds - isolation & purification</subject><subject>Plant Extracts - chemistry</subject><subject>Plant physiology and development</subject><subject>Plant Roots - chemistry</subject><subject>Potassium - analysis</subject><subject>Potassium - chemistry</subject><subject>Potassium - isolation & purification</subject><subject>Specimen Handling</subject><subject>Spectrophotometry, Atomic</subject><subject>Zinc - analysis</subject><subject>Zinc - chemistry</subject><subject>Zinc - isolation & purification</subject><issn>0937-0633</issn><issn>1432-1130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpV0FFLwzAQB_AgipvTR1-lL_pWvUuapHnUoU4Y-KLPJU0TqLRLTTLQb2_nKkMO7iD5cRx_Qi4RbhFA3kUAigJwLC6OyBwLRnNEBsdkDorJHARjM3IW4wfsqKKnZIYohSoB5-Th0TlrUuZdZr9S0Ca1fpMNq2zsvU26y-JgTat_n9vxp9OblAXv05-P5-TE6S7ai2kuyPvT49tyla9fn1-W9-vcMFQpN2AaIwqopaGNKJmkhSx5w52DmpZ16ZBSWRYF45QqpngJXNVaNUKKBrChbEFu9nuH4D-3Nqaqb6Ox3XiR9dtYScok4wUfYb6HJvgYg3XVENpeh-8KodqFVv0LbfRX0-Jt3dvmoKeURnA9AR2N7lzQG9PGgysQqGSC_QB1InGw</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>WEBER, G</creator><creator>MESSERSCHMIDT, J</creator><creator>VON BOHLEN, A</creator><creator>ALT, F</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>Effect of extraction pH on metal speciation in plant root extracts</title><author>WEBER, G ; MESSERSCHMIDT, J ; VON BOHLEN, A ; ALT, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-c0cdc640b7c2d683724785d5ff0b28b8f1227844352293958059ba9d676d01d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Calcium - analysis</topic><topic>Calcium - chemistry</topic><topic>Calcium - isolation & purification</topic><topic>Chemical constitution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Iron - analysis</topic><topic>Iron - chemistry</topic><topic>Iron - isolation & purification</topic><topic>Manganese - analysis</topic><topic>Manganese - chemistry</topic><topic>Manganese - isolation & purification</topic><topic>Metals - analysis</topic><topic>Metals - chemistry</topic><topic>Metals - isolation & purification</topic><topic>Organometallic Compounds - analysis</topic><topic>Organometallic Compounds - chemistry</topic><topic>Organometallic Compounds - isolation & purification</topic><topic>Plant Extracts - chemistry</topic><topic>Plant physiology and development</topic><topic>Plant Roots - chemistry</topic><topic>Potassium - analysis</topic><topic>Potassium - chemistry</topic><topic>Potassium - isolation & purification</topic><topic>Specimen Handling</topic><topic>Spectrophotometry, Atomic</topic><topic>Zinc - analysis</topic><topic>Zinc - chemistry</topic><topic>Zinc - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WEBER, G</creatorcontrib><creatorcontrib>MESSERSCHMIDT, J</creatorcontrib><creatorcontrib>VON BOHLEN, A</creatorcontrib><creatorcontrib>ALT, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fresenius' journal of analytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WEBER, G</au><au>MESSERSCHMIDT, J</au><au>VON BOHLEN, A</au><au>ALT, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of extraction pH on metal speciation in plant root extracts</atitle><jtitle>Fresenius' journal of analytical chemistry</jtitle><addtitle>Fresenius J Anal Chem</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>371</volume><issue>7</issue><spage>921</spage><epage>926</epage><pages>921-926</pages><issn>0937-0633</issn><eissn>1432-1130</eissn><abstract>The species patterns of nutrient and trace metals (K, Ca, Mg, Mn, Fe, Zn) obtained by extraction of plant roots have been determined as a function of extraction pH in the range 4-9. The extractable metal concentrations were subdivided into low-molecular-weight (<10 kDa) and high-molecular-weight (>10 kDa) metal species by TXRF analysis. Except for pH 9, the low-molecular-weight fraction is predominant. This fraction was further separated by HPLC with AAS detection. It was demonstrated that the nature of the metal species changes dramatically when the extraction pH is changed, especially in the range 9-7. Information about the chemical nature of chromatographically separated metal species is obtained by use of different electrochemical detectors (amperometric detection at a glassy carbon or copper electrode and pulsed amperometric detection) and diode-array UV detection.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11769801</pmid><doi>10.1007/s002160101056</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0937-0633 |
| ispartof | Fresenius' journal of analytical chemistry, 2001-12, Vol.371 (7), p.921-926 |
| issn | 0937-0633 1432-1130 |
| language | eng |
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| subjects | Biological and medical sciences Calcium - analysis Calcium - chemistry Calcium - isolation & purification Chemical constitution Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Iron - analysis Iron - chemistry Iron - isolation & purification Manganese - analysis Manganese - chemistry Manganese - isolation & purification Metals - analysis Metals - chemistry Metals - isolation & purification Organometallic Compounds - analysis Organometallic Compounds - chemistry Organometallic Compounds - isolation & purification Plant Extracts - chemistry Plant physiology and development Plant Roots - chemistry Potassium - analysis Potassium - chemistry Potassium - isolation & purification Specimen Handling Spectrophotometry, Atomic Zinc - analysis Zinc - chemistry Zinc - isolation & purification |
| title | Effect of extraction pH on metal speciation in plant root extracts |
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