Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions
The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron micro...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 2001-12, Vol.509 (3), p.417-422 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 422 |
|---|---|
| container_issue | 3 |
| container_start_page | 417 |
| container_title | FEBS letters |
| container_volume | 509 |
| creator | Moreau, C Cavalier, A Le Floch, M Segalen, J Rocher, C Traïkia, M Leray, G Bondon, A Thomas, D Le Rumeur, E |
| description | The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and (31)P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in-vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin-lattice T(1) relaxation times). Using broad-band and magic angle spinning (31)P NMR spectroscopy with lanthanide ions (Pr(3+)), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans-bilayer distribution is highly asymmetrical. |
| doi_str_mv | 10.1016/S0014-5793(01)03199-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_72371986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72371986</sourcerecordid><originalsourceid>FETCH-LOGICAL-p124t-122a4fca762024fbe50239ece4c61993bd23c4ab50d9e5174d3f48085439b22d3</originalsourceid><addsrcrecordid>eNo9kMtOwzAQRb0A0VL4BJBXiC4CfiWpl6jiJZWHKKwjx3ZaozgOtgPqjk_HooXFaDQzV1f3DAAnGF1ghIvLJUKYZXnJ6TnCU0Qx5xnbA-P_9QgchvCO0jzD_ACMMC4Z50UxBt9L4aVrtbUC9msXUrWmNwqG6AcZB6-h6T51iGYlolaw3kBj7dC5lWsV1K2W0bsOWiO9C9L1Gyg6Bc8pnj7Dx4cXGPpfxfb0ZeIatqKLa9EZlZxdF47AfiPaoI93fQLebq5f53fZ4un2fn61yHpMWMwwIYI1UpQFQYQ1tc4RoVxLzWSRcGmtCJVM1DlSXOcJT9GGzdAsZ5TXhCg6AWdb3967jyEBVdYEqdsUR7shVCWhJeazIglPd8KhtlpVvTdW-E319zP6A-8Wb6M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72371986</pqid></control><display><type>article</type><title>Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>Access via ScienceDirect (Elsevier)</source><source>Wiley Online Library (Open Access Collection)</source><source>Alma/SFX Local Collection</source><creator>Moreau, C ; Cavalier, A ; Le Floch, M ; Segalen, J ; Rocher, C ; Traïkia, M ; Leray, G ; Bondon, A ; Thomas, D ; Le Rumeur, E</creator><creatorcontrib>Moreau, C ; Cavalier, A ; Le Floch, M ; Segalen, J ; Rocher, C ; Traïkia, M ; Leray, G ; Bondon, A ; Thomas, D ; Le Rumeur, E</creatorcontrib><description>The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and (31)P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in-vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin-lattice T(1) relaxation times). Using broad-band and magic angle spinning (31)P NMR spectroscopy with lanthanide ions (Pr(3+)), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans-bilayer distribution is highly asymmetrical.</description><identifier>ISSN: 0014-5793</identifier><identifier>DOI: 10.1016/S0014-5793(01)03199-4</identifier><identifier>PMID: 11749966</identifier><language>eng</language><publisher>England</publisher><subject>Cell Polarity ; Immunohistochemistry - methods ; Lanthanoid Series Elements - metabolism ; Lipid Bilayers - chemistry ; Magnetic Resonance Spectroscopy - methods ; Microscopy, Electron - methods ; Phospholipids - chemistry ; Radioisotopes ; Sarcolemma - chemistry ; Sarcolemma - ultrastructure ; Time Factors</subject><ispartof>FEBS letters, 2001-12, Vol.509 (3), p.417-422</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11749966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moreau, C</creatorcontrib><creatorcontrib>Cavalier, A</creatorcontrib><creatorcontrib>Le Floch, M</creatorcontrib><creatorcontrib>Segalen, J</creatorcontrib><creatorcontrib>Rocher, C</creatorcontrib><creatorcontrib>Traïkia, M</creatorcontrib><creatorcontrib>Leray, G</creatorcontrib><creatorcontrib>Bondon, A</creatorcontrib><creatorcontrib>Thomas, D</creatorcontrib><creatorcontrib>Le Rumeur, E</creatorcontrib><title>Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and (31)P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in-vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin-lattice T(1) relaxation times). Using broad-band and magic angle spinning (31)P NMR spectroscopy with lanthanide ions (Pr(3+)), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans-bilayer distribution is highly asymmetrical.</description><subject>Cell Polarity</subject><subject>Immunohistochemistry - methods</subject><subject>Lanthanoid Series Elements - metabolism</subject><subject>Lipid Bilayers - chemistry</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Microscopy, Electron - methods</subject><subject>Phospholipids - chemistry</subject><subject>Radioisotopes</subject><subject>Sarcolemma - chemistry</subject><subject>Sarcolemma - ultrastructure</subject><subject>Time Factors</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRb0A0VL4BJBXiC4CfiWpl6jiJZWHKKwjx3ZaozgOtgPqjk_HooXFaDQzV1f3DAAnGF1ghIvLJUKYZXnJ6TnCU0Qx5xnbA-P_9QgchvCO0jzD_ACMMC4Z50UxBt9L4aVrtbUC9msXUrWmNwqG6AcZB6-h6T51iGYlolaw3kBj7dC5lWsV1K2W0bsOWiO9C9L1Gyg6Bc8pnj7Dx4cXGPpfxfb0ZeIatqKLa9EZlZxdF47AfiPaoI93fQLebq5f53fZ4un2fn61yHpMWMwwIYI1UpQFQYQ1tc4RoVxLzWSRcGmtCJVM1DlSXOcJT9GGzdAsZ5TXhCg6AWdb3967jyEBVdYEqdsUR7shVCWhJeazIglPd8KhtlpVvTdW-E319zP6A-8Wb6M</recordid><startdate>20011214</startdate><enddate>20011214</enddate><creator>Moreau, C</creator><creator>Cavalier, A</creator><creator>Le Floch, M</creator><creator>Segalen, J</creator><creator>Rocher, C</creator><creator>Traïkia, M</creator><creator>Leray, G</creator><creator>Bondon, A</creator><creator>Thomas, D</creator><creator>Le Rumeur, E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20011214</creationdate><title>Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions</title><author>Moreau, C ; Cavalier, A ; Le Floch, M ; Segalen, J ; Rocher, C ; Traïkia, M ; Leray, G ; Bondon, A ; Thomas, D ; Le Rumeur, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-122a4fca762024fbe50239ece4c61993bd23c4ab50d9e5174d3f48085439b22d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Cell Polarity</topic><topic>Immunohistochemistry - methods</topic><topic>Lanthanoid Series Elements - metabolism</topic><topic>Lipid Bilayers - chemistry</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Microscopy, Electron - methods</topic><topic>Phospholipids - chemistry</topic><topic>Radioisotopes</topic><topic>Sarcolemma - chemistry</topic><topic>Sarcolemma - ultrastructure</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moreau, C</creatorcontrib><creatorcontrib>Cavalier, A</creatorcontrib><creatorcontrib>Le Floch, M</creatorcontrib><creatorcontrib>Segalen, J</creatorcontrib><creatorcontrib>Rocher, C</creatorcontrib><creatorcontrib>Traïkia, M</creatorcontrib><creatorcontrib>Leray, G</creatorcontrib><creatorcontrib>Bondon, A</creatorcontrib><creatorcontrib>Thomas, D</creatorcontrib><creatorcontrib>Le Rumeur, E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moreau, C</au><au>Cavalier, A</au><au>Le Floch, M</au><au>Segalen, J</au><au>Rocher, C</au><au>Traïkia, M</au><au>Leray, G</au><au>Bondon, A</au><au>Thomas, D</au><au>Le Rumeur, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2001-12-14</date><risdate>2001</risdate><volume>509</volume><issue>3</issue><spage>417</spage><epage>422</epage><pages>417-422</pages><issn>0014-5793</issn><abstract>The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and (31)P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in-vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin-lattice T(1) relaxation times). Using broad-band and magic angle spinning (31)P NMR spectroscopy with lanthanide ions (Pr(3+)), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans-bilayer distribution is highly asymmetrical.</abstract><cop>England</cop><pmid>11749966</pmid><doi>10.1016/S0014-5793(01)03199-4</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2001-12, Vol.509 (3), p.417-422 |
| issn | 0014-5793 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72371986 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Cell Polarity Immunohistochemistry - methods Lanthanoid Series Elements - metabolism Lipid Bilayers - chemistry Magnetic Resonance Spectroscopy - methods Microscopy, Electron - methods Phospholipids - chemistry Radioisotopes Sarcolemma - chemistry Sarcolemma - ultrastructure Time Factors |
| title | Sarcolemma phospholipid structure investigated by immunogold electron microscopy and (31)P NMR spectroscopy with lanthanide ions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T19%3A52%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sarcolemma%20phospholipid%20structure%20investigated%20by%20immunogold%20electron%20microscopy%20and%20(31)P%20NMR%20spectroscopy%20with%20lanthanide%20ions&rft.jtitle=FEBS%20letters&rft.au=Moreau,%20C&rft.date=2001-12-14&rft.volume=509&rft.issue=3&rft.spage=417&rft.epage=422&rft.pages=417-422&rft.issn=0014-5793&rft_id=info:doi/10.1016/S0014-5793(01)03199-4&rft_dat=%3Cproquest_pubme%3E72371986%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72371986&rft_id=info:pmid/11749966&rfr_iscdi=true |