Comparison of in vitro immunostimulatory potential of live and inactivated influenza viruses
Live influenza viruses, heat-inactivated virus, and a trivalent formalin-inactivated influenza vaccine were analyzed for their in vitro stimulatory properties on immune cells from healthy donors. Lymphocyte proliferation induced by each influenza antigen was comparable. Influenza vaccine stimulated...
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| Veröffentlicht in: | Human immunology 2000-09, Vol.61 (9), p.845-849 |
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| creator | Blazevic, Vesna Mac Trubey, C Shearer, Gene M |
| description | Live influenza viruses, heat-inactivated virus, and a trivalent formalin-inactivated influenza vaccine were analyzed for their
in vitro stimulatory properties on immune cells from healthy donors. Lymphocyte proliferation induced by each influenza antigen was comparable. Influenza vaccine stimulated significantly lower production of interferon-γ (IFN-γ) compared with live and heat inactivated viruses, whereas both vaccine and heat-inactivated influenza induced lower levels of IFN-α compared with live virus. Furthermore, only live virus generated influenza-specific cytotoxic T lymphocyte (CTL) activity. A significant increase in monocyte expression of CD80, CD86, CD40, and human leukocyte antigen-DR (HLA-DR) was also induced by live influenza virus. Our results suggest that immunization with live influenza vaccines might induce immune responses that would not be induced by conventional inactivated vaccines, including CTL generation, antiviral IFN-γ and IFN-α cytokine production, and increased antigen presentation and costimulatory capacity on antigen presenting cells (APC). |
| doi_str_mv | 10.1016/S0198-8859(00)00170-1 |
| format | Article |
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in vitro stimulatory properties on immune cells from healthy donors. Lymphocyte proliferation induced by each influenza antigen was comparable. Influenza vaccine stimulated significantly lower production of interferon-γ (IFN-γ) compared with live and heat inactivated viruses, whereas both vaccine and heat-inactivated influenza induced lower levels of IFN-α compared with live virus. Furthermore, only live virus generated influenza-specific cytotoxic T lymphocyte (CTL) activity. A significant increase in monocyte expression of CD80, CD86, CD40, and human leukocyte antigen-DR (HLA-DR) was also induced by live influenza virus. 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in vitro stimulatory properties on immune cells from healthy donors. Lymphocyte proliferation induced by each influenza antigen was comparable. Influenza vaccine stimulated significantly lower production of interferon-γ (IFN-γ) compared with live and heat inactivated viruses, whereas both vaccine and heat-inactivated influenza induced lower levels of IFN-α compared with live virus. Furthermore, only live virus generated influenza-specific cytotoxic T lymphocyte (CTL) activity. A significant increase in monocyte expression of CD80, CD86, CD40, and human leukocyte antigen-DR (HLA-DR) was also induced by live influenza virus. Our results suggest that immunization with live influenza vaccines might induce immune responses that would not be induced by conventional inactivated vaccines, including CTL generation, antiviral IFN-γ and IFN-α cytokine production, and increased antigen presentation and costimulatory capacity on antigen presenting cells (APC).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11053627</pmid><doi>10.1016/S0198-8859(00)00170-1</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0198-8859 |
| ispartof | Human immunology, 2000-09, Vol.61 (9), p.845-849 |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | a-Interferon Antigens, CD - biosynthesis B7-1 Antigen - biosynthesis B7-2 Antigen CD40 Antigens - biosynthesis Cell Division Cells, Cultured costimulatory molecules g-Interferon Heating HLA-DR Antigens - biosynthesis Humans influenza Influenza A virus - immunology Influenza Vaccines - immunology influenza virus Interferon-alpha - biosynthesis Interferon-gamma - biosynthesis interferon-α interferon-γ Membrane Glycoproteins - biosynthesis Monocytes - immunology T-Lymphocytes, Cytotoxic - immunology vaccine Vaccines, Attenuated - immunology |
| title | Comparison of in vitro immunostimulatory potential of live and inactivated influenza viruses |
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