Scanning Electrochemical Microscopy. 40. Voltammetric Ion-Selective Micropipet Electrodes for Probing Ion Transfer at Bilayer Lipid Membranes
Voltammetric ion-selective micropipet electrodes for use in scanning electrochemical microscopy (SECM) for detection of potassium ion were fabricated. These used pulled borosilicate capillaries with tip orifice radii of 0.7−20 μm with silanized inner walls filled with a solution of 10 mM valinomycin...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2000-10, Vol.72 (20), p.4940-4948 |
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| creator | Amemiya, Shigeru Bard, Allen J |
| description | Voltammetric ion-selective micropipet electrodes for use in scanning electrochemical microscopy (SECM) for detection of potassium ion were fabricated. These used pulled borosilicate capillaries with tip orifice radii of 0.7−20 μm with silanized inner walls filled with a solution of 10 mM valinomycin and 10 mM ETH 500 in dichloroethane. The electrodes were characterized by determining the steady-state tip current for K+ concentrations of 0.05−0.3 mM. The tips were used in the SECM feedback and generation−collection modes to study K+ transfer through gramicidin channels in a horizontal bilayer lipid membrane (glycerol monooleate). |
| doi_str_mv | 10.1021/ac0004207 |
| format | Article |
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Voltammetric Ion-Selective Micropipet Electrodes for Probing Ion Transfer at Bilayer Lipid Membranes</title><author>Amemiya, Shigeru ; Bard, Allen J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a404t-3f8e0a75ecf6de47357cced1ac112c85003e0890de649afbe6cce165a5bc73463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Electrochemical methods</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Electrodes: preparations and properties</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Ion selective electrodes</topic><topic>Ions</topic><topic>Lipid Bilayers</topic><topic>Lipids</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Potassium</topic><topic>Spectrum analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amemiya, Shigeru</creatorcontrib><creatorcontrib>Bard, Allen J</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amemiya, Shigeru</au><au>Bard, Allen J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scanning Electrochemical Microscopy. 40. Voltammetric Ion-Selective Micropipet Electrodes for Probing Ion Transfer at Bilayer Lipid Membranes</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2000-10-15</date><risdate>2000</risdate><volume>72</volume><issue>20</issue><spage>4940</spage><epage>4948</epage><pages>4940-4948</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Voltammetric ion-selective micropipet electrodes for use in scanning electrochemical microscopy (SECM) for detection of potassium ion were fabricated. These used pulled borosilicate capillaries with tip orifice radii of 0.7−20 μm with silanized inner walls filled with a solution of 10 mM valinomycin and 10 mM ETH 500 in dichloroethane. The electrodes were characterized by determining the steady-state tip current for K+ concentrations of 0.05−0.3 mM. 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| subjects | Analytical chemistry Chemistry Electrochemical methods Electrochemistry Electrodes Electrodes: preparations and properties Exact sciences and technology General and physical chemistry Ion selective electrodes Ions Lipid Bilayers Lipids Microscopy, Electron, Scanning - methods Potassium Spectrum analysis |
| title | Scanning Electrochemical Microscopy. 40. Voltammetric Ion-Selective Micropipet Electrodes for Probing Ion Transfer at Bilayer Lipid Membranes |
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