Tissue Factor Encryption/de-encryption Is not Altered in the Absence of the Cytoplasmic Domain
Summary Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quanti...
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| Veröffentlicht in: | Thrombosis and haemostasis 2000-10, Vol.84 (4), p.657-663 |
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| container_title | Thrombosis and haemostasis |
| container_volume | 84 |
| creator | Carson, Steven D. Bromberg, Michael E. |
| description | Summary
Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF
CD
). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF
CD
were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF
CD
were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain. |
| doi_str_mv | 10.1055/s-0037-1614083 |
| format | Article |
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Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF
CD
). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF
CD
were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF
CD
were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.</description><identifier>ISSN: 0340-6245</identifier><identifier>EISSN: 2567-689X</identifier><identifier>DOI: 10.1055/s-0037-1614083</identifier><identifier>PMID: 11057866</identifier><identifier>CODEN: THHADQ</identifier><language>eng</language><publisher>Stuttgart: Schattauer Verlag für Medizin und Naturwissenschaften</publisher><subject>Animals ; Biological and medical sciences ; Blood coagulation. Blood cells ; Cell Line ; Coagulation factors ; Fundamental and applied biological sciences. Psychology ; Humans ; Mice ; Molecular and cellular biology ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Review Article ; Structure-Activity Relationship ; Thromboplastin - chemistry ; Thromboplastin - genetics ; Thromboplastin - metabolism ; Transfection</subject><ispartof>Thrombosis and haemostasis, 2000-10, Vol.84 (4), p.657-663</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-c9bbd9a6df6d193d6fecd2fc48217410819be6a7d7a1696503c0c7a77de8b7993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0037-1614083.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-0037-1614083$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,27901,27902,54534,54535</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1536351$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11057866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carson, Steven D.</creatorcontrib><creatorcontrib>Bromberg, Michael E.</creatorcontrib><title>Tissue Factor Encryption/de-encryption Is not Altered in the Absence of the Cytoplasmic Domain</title><title>Thrombosis and haemostasis</title><addtitle>Thromb Haemost</addtitle><description>Summary
Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF
CD
). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF
CD
were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF
CD
were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Cell Line</subject><subject>Coagulation factors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Review Article</subject><subject>Structure-Activity Relationship</subject><subject>Thromboplastin - chemistry</subject><subject>Thromboplastin - genetics</subject><subject>Thromboplastin - metabolism</subject><subject>Transfection</subject><issn>0340-6245</issn><issn>2567-689X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqtkkFr3DAQhU1pabZprz0WHUpvTiTLluzjsk3aQKCXFHqqkKUxVrAlVyOn7L-vNrskUOitp-Ex37wHTyqK94xeMNo0l1hSymXJBKtpy18Um6oRshRt9-NlsaG8pqWo6uaseIN4TykTdde8Ls5YvpWtEJvi551DXIFca5NCJFfexP2SXPCXFkp4UuQGiQ-JbKcEESxxnqQRyLbHzAAJw6Pc7VNYJo2zM-RzmLXzb4tXg54Q3p3mefH9-upu97W8_fblZre9LU1Tt6k0Xd_bTgs7CMs6bsUAxlaDqduKyZrRlnU9CC2t1Ex0oqHcUCO1lBbaXnYdPy8-HX2XGH6tgEnNDg1Mk_YQVlSy4pJVrM3gxRE0MSBGGNQS3azjXjGqDo0qVIdG1anRfPDh5Lz2M9hn_FRhBj6eAI1GT0PU3jh85houeMMyVh6xNDqYQd2HNfpcyb9z3ZFHM-qU9ArxyTONMcx9wByjvVWjhjlg0gdtgk_gU15EM7oHUI_Pq9qOqln7FU10S1L5k1QKx_BbjWmecpb5j1m4gHF6-juP_wEAEd2A</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Carson, Steven D.</creator><creator>Bromberg, Michael E.</creator><general>Schattauer Verlag für Medizin und Naturwissenschaften</general><general>Schattauer GmbH</general><general>Schattauer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Tissue Factor Encryption/de-encryption Is not Altered in the Absence of the Cytoplasmic Domain</title><author>Carson, Steven D. ; Bromberg, Michael E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-c9bbd9a6df6d193d6fecd2fc48217410819be6a7d7a1696503c0c7a77de8b7993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Cell Line</topic><topic>Coagulation factors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Review Article</topic><topic>Structure-Activity Relationship</topic><topic>Thromboplastin - chemistry</topic><topic>Thromboplastin - genetics</topic><topic>Thromboplastin - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carson, Steven D.</creatorcontrib><creatorcontrib>Bromberg, Michael E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis and haemostasis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carson, Steven D.</au><au>Bromberg, Michael E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue Factor Encryption/de-encryption Is not Altered in the Absence of the Cytoplasmic Domain</atitle><jtitle>Thrombosis and haemostasis</jtitle><addtitle>Thromb Haemost</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>84</volume><issue>4</issue><spage>657</spage><epage>663</epage><pages>657-663</pages><issn>0340-6245</issn><eissn>2567-689X</eissn><coden>THHADQ</coden><abstract>Summary
Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SIT1, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF
CD
). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF
CD
were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF
CD
were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.</abstract><cop>Stuttgart</cop><pub>Schattauer Verlag für Medizin und Naturwissenschaften</pub><pmid>11057866</pmid><doi>10.1055/s-0037-1614083</doi><tpages>7</tpages></addata></record> |
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| ispartof | Thrombosis and haemostasis, 2000-10, Vol.84 (4), p.657-663 |
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| language | eng |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | Animals Biological and medical sciences Blood coagulation. Blood cells Cell Line Coagulation factors Fundamental and applied biological sciences. Psychology Humans Mice Molecular and cellular biology Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Review Article Structure-Activity Relationship Thromboplastin - chemistry Thromboplastin - genetics Thromboplastin - metabolism Transfection |
| title | Tissue Factor Encryption/de-encryption Is not Altered in the Absence of the Cytoplasmic Domain |
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