A Genetically Encoded Ratiometric Indicator for Chloride: Capturing Chloride Transients in Cultured Hippocampal Neurons

We constructed a novel optical indicator for chloride ions by fusing the chloride-sensitive yellow fluorescent protein with the chloride-insensitive cyan fluorescent protein. The ratio of FRET-dependent emission of these fluorophores varied in proportion to the concentration of Cl − and was used to...

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Veröffentlicht in:	Neuron (Cambridge, Mass.) Mass.), 2000-09, Vol.27 (3), p.447-459
Hauptverfasser:	Kuner, Thomas, Augustine, George J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Cells, Cultured Chlorides - analysis Chlorides - metabolism Dendrites - metabolism Energy Transfer Fluorescent Dyes gamma-Aminobutyric Acid - metabolism Hippocampus - cytology Hippocampus - metabolism Hydrogen-Ion Concentration Intracellular Fluid - chemistry Intracellular Fluid - metabolism Luminescent Proteins - genetics Luminescent Proteins - metabolism Neurons - cytology Neurons - metabolism Rats Rats, Sprague-Dawley Receptors, GABA-A - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Signal Transduction - physiology Spectrometry, Fluorescence Synapses - metabolism Synaptic Transmission - physiology Transfection
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