unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration
Nuclear migration plays an essential role in the growth and development of a wide variety of eukaryotes. Mutations in unc-84, which encodes a conserved component of the nuclear envelope, have been shown to disrupt nuclear migration in two C. elegans tissues. We show that mutations in unc-83 disrupt...
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| Veröffentlicht in: | Development (Cambridge) 2001-12, Vol.128 (24), p.5039-5050 |
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| creator | Starr, D A Hermann, G J Malone, C J Fixsen, W Priess, J R Horvitz, H R Han, M |
| description | Nuclear migration plays an essential role in the growth and development of a wide variety of eukaryotes. Mutations in unc-84, which encodes a conserved component of the nuclear envelope, have been shown to disrupt nuclear migration in two C. elegans tissues. We show that mutations in unc-83 disrupt nuclear migration in a similar manner in migrating P cells, hyp7 precursors and the intestinal primordium, but have no obvious defects in the association of centrosomes with nuclei or the structure of the nuclear lamina of migrating nuclei. We also show that unc-83 encodes a novel transmembrane protein. We identified three unc-83 transcripts that are expressed in a tissue-specific manner. Antibodies against UNC-83 co-localized to the nuclear envelope with lamin and UNC-84. Unlike UNC-84, UNC-83 localized to only specific nuclei, many of which were migratory. UNC-83 failed to localize to the nuclear envelope in unc-84 mutants with lesions in the conserved SUN domain of UNC-84, and UNC-83 interacted with the SUN domain of UNC-84 in vitro, suggesting that these two proteins function together during nuclear migration. We favor a model in which UNC-84 directly recruits UNC-83 to the nuclear envelope where they help transfer force between the cytoskeleton and the nucleus. |
| doi_str_mv | 10.1242/dev.128.24.5039 |
| format | Article |
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Mutations in unc-84, which encodes a conserved component of the nuclear envelope, have been shown to disrupt nuclear migration in two C. elegans tissues. We show that mutations in unc-83 disrupt nuclear migration in a similar manner in migrating P cells, hyp7 precursors and the intestinal primordium, but have no obvious defects in the association of centrosomes with nuclei or the structure of the nuclear lamina of migrating nuclei. We also show that unc-83 encodes a novel transmembrane protein. We identified three unc-83 transcripts that are expressed in a tissue-specific manner. Antibodies against UNC-83 co-localized to the nuclear envelope with lamin and UNC-84. Unlike UNC-84, UNC-83 localized to only specific nuclei, many of which were migratory. UNC-83 failed to localize to the nuclear envelope in unc-84 mutants with lesions in the conserved SUN domain of UNC-84, and UNC-83 interacted with the SUN domain of UNC-84 in vitro, suggesting that these two proteins function together during nuclear migration. We favor a model in which UNC-84 directly recruits UNC-83 to the nuclear envelope where they help transfer force between the cytoskeleton and the nucleus.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.128.24.5039</identifier><identifier>PMID: 11748140</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Active Transport, Cell Nucleus ; Animals ; Caenorhabditis elegans ; Caenorhabditis elegans Proteins ; Cell Compartmentation ; Cell Nucleus - physiology ; Centrosome - physiology ; centrosomes ; Cloning, Molecular ; Intestines - embryology ; Membrane Glycoproteins - isolation & purification ; Membrane Proteins - genetics ; Membrane Proteins - isolation & purification ; Microtubules - physiology ; Movement - physiology ; Nuclear Envelope - chemistry ; Nuclear Envelope - physiology ; Nuclear Matrix - physiology ; Nuclear Proteins - genetics ; Nuclear Proteins - isolation & purification ; RNA, Antisense ; RNA, Small Interfering ; UNC-83 protein ; unc-84 gene ; UNC-84 protein</subject><ispartof>Development (Cambridge), 2001-12, Vol.128 (24), p.5039-5050</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-a9b448e051d2bda6303581c0c7d1fe713fe383291916fe02d7052fe14ae6cbb93</citedby><cites>FETCH-LOGICAL-c363t-a9b448e051d2bda6303581c0c7d1fe713fe383291916fe02d7052fe14ae6cbb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11748140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Starr, D A</creatorcontrib><creatorcontrib>Hermann, G J</creatorcontrib><creatorcontrib>Malone, C J</creatorcontrib><creatorcontrib>Fixsen, W</creatorcontrib><creatorcontrib>Priess, J R</creatorcontrib><creatorcontrib>Horvitz, H R</creatorcontrib><creatorcontrib>Han, M</creatorcontrib><title>unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>Nuclear migration plays an essential role in the growth and development of a wide variety of eukaryotes. Mutations in unc-84, which encodes a conserved component of the nuclear envelope, have been shown to disrupt nuclear migration in two C. elegans tissues. We show that mutations in unc-83 disrupt nuclear migration in a similar manner in migrating P cells, hyp7 precursors and the intestinal primordium, but have no obvious defects in the association of centrosomes with nuclei or the structure of the nuclear lamina of migrating nuclei. We also show that unc-83 encodes a novel transmembrane protein. We identified three unc-83 transcripts that are expressed in a tissue-specific manner. Antibodies against UNC-83 co-localized to the nuclear envelope with lamin and UNC-84. Unlike UNC-84, UNC-83 localized to only specific nuclei, many of which were migratory. UNC-83 failed to localize to the nuclear envelope in unc-84 mutants with lesions in the conserved SUN domain of UNC-84, and UNC-83 interacted with the SUN domain of UNC-84 in vitro, suggesting that these two proteins function together during nuclear migration. We favor a model in which UNC-84 directly recruits UNC-83 to the nuclear envelope where they help transfer force between the cytoskeleton and the nucleus.</description><subject>Active Transport, Cell Nucleus</subject><subject>Animals</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans Proteins</subject><subject>Cell Compartmentation</subject><subject>Cell Nucleus - physiology</subject><subject>Centrosome - physiology</subject><subject>centrosomes</subject><subject>Cloning, Molecular</subject><subject>Intestines - embryology</subject><subject>Membrane Glycoproteins - isolation & purification</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Microtubules - physiology</subject><subject>Movement - physiology</subject><subject>Nuclear Envelope - chemistry</subject><subject>Nuclear Envelope - physiology</subject><subject>Nuclear Matrix - physiology</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>RNA, Antisense</subject><subject>RNA, Small Interfering</subject><subject>UNC-83 protein</subject><subject>unc-84 gene</subject><subject>UNC-84 protein</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo7rh69iY5eevZVJLuJEdZ_IIFL3oO6XRlJtKdjEn3iv-9GWZQPHmqB_WrR_EeIa-B7YFLfjfhYxN6z-W-Z8I8ITuQSnUGuHlKdsz0rANj4Ia8qPU7Y0wMSj0nNwBKapBsR8KWfKcFxeTzhJU6mvIjztTn5ZQTppXmQNcj0rT5GV1pYFvnE1KXJhorxVobFd1MQy70VNqq_IGXeChujTm9JM-Cmyu-us5b8u3D-6_3n7qHLx8_37976LwYxNo5M0qpkfUw8XFyg2Ci1-CZVxMEVCACCi24AQNDQMYnxXoeEKTDwY-jEbfk7cW3PfJjw7raJVaP8-wS5q1axcXQA4j_gqC51przBt5dQF9yrQWDPZW4uPLLArPnDmzroAltubTnDtrFm6v1Ni44_eWvoTdgfwGO8XD8GQvaMeY5H2Jd69ntnO8_jr8BYl6THg</recordid><startdate>20011215</startdate><enddate>20011215</enddate><creator>Starr, D A</creator><creator>Hermann, G J</creator><creator>Malone, C J</creator><creator>Fixsen, W</creator><creator>Priess, J R</creator><creator>Horvitz, H R</creator><creator>Han, M</creator><general>The Company of Biologists Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20011215</creationdate><title>unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration</title><author>Starr, D A ; Hermann, G J ; Malone, C J ; Fixsen, W ; Priess, J R ; Horvitz, H R ; Han, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-a9b448e051d2bda6303581c0c7d1fe713fe383291916fe02d7052fe14ae6cbb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Animals</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans Proteins</topic><topic>Cell Compartmentation</topic><topic>Cell Nucleus - physiology</topic><topic>Centrosome - physiology</topic><topic>centrosomes</topic><topic>Cloning, Molecular</topic><topic>Intestines - embryology</topic><topic>Membrane Glycoproteins - isolation & purification</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Microtubules - physiology</topic><topic>Movement - physiology</topic><topic>Nuclear Envelope - chemistry</topic><topic>Nuclear Envelope - physiology</topic><topic>Nuclear Matrix - physiology</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>RNA, Antisense</topic><topic>RNA, Small Interfering</topic><topic>UNC-83 protein</topic><topic>unc-84 gene</topic><topic>UNC-84 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Starr, D A</creatorcontrib><creatorcontrib>Hermann, G J</creatorcontrib><creatorcontrib>Malone, C J</creatorcontrib><creatorcontrib>Fixsen, W</creatorcontrib><creatorcontrib>Priess, J R</creatorcontrib><creatorcontrib>Horvitz, H R</creatorcontrib><creatorcontrib>Han, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Starr, D A</au><au>Hermann, G J</au><au>Malone, C J</au><au>Fixsen, W</au><au>Priess, J R</au><au>Horvitz, H R</au><au>Han, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2001-12-15</date><risdate>2001</risdate><volume>128</volume><issue>24</issue><spage>5039</spage><epage>5050</epage><pages>5039-5050</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Nuclear migration plays an essential role in the growth and development of a wide variety of eukaryotes. Mutations in unc-84, which encodes a conserved component of the nuclear envelope, have been shown to disrupt nuclear migration in two C. elegans tissues. We show that mutations in unc-83 disrupt nuclear migration in a similar manner in migrating P cells, hyp7 precursors and the intestinal primordium, but have no obvious defects in the association of centrosomes with nuclei or the structure of the nuclear lamina of migrating nuclei. We also show that unc-83 encodes a novel transmembrane protein. We identified three unc-83 transcripts that are expressed in a tissue-specific manner. Antibodies against UNC-83 co-localized to the nuclear envelope with lamin and UNC-84. Unlike UNC-84, UNC-83 localized to only specific nuclei, many of which were migratory. UNC-83 failed to localize to the nuclear envelope in unc-84 mutants with lesions in the conserved SUN domain of UNC-84, and UNC-83 interacted with the SUN domain of UNC-84 in vitro, suggesting that these two proteins function together during nuclear migration. We favor a model in which UNC-84 directly recruits UNC-83 to the nuclear envelope where they help transfer force between the cytoskeleton and the nucleus.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>11748140</pmid><doi>10.1242/dev.128.24.5039</doi><tpages>12</tpages></addata></record> |
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| ispartof | Development (Cambridge), 2001-12, Vol.128 (24), p.5039-5050 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Active Transport, Cell Nucleus Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Cell Compartmentation Cell Nucleus - physiology Centrosome - physiology centrosomes Cloning, Molecular Intestines - embryology Membrane Glycoproteins - isolation & purification Membrane Proteins - genetics Membrane Proteins - isolation & purification Microtubules - physiology Movement - physiology Nuclear Envelope - chemistry Nuclear Envelope - physiology Nuclear Matrix - physiology Nuclear Proteins - genetics Nuclear Proteins - isolation & purification RNA, Antisense RNA, Small Interfering UNC-83 protein unc-84 gene UNC-84 protein |
| title | unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration |
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