THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE
Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 1...
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| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2000-11, Vol.12 (11), p.1669-1675 |
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| creator | Durbin, Elizabeth A Gregory, Meredith S Messingham, Kelly A.N Fontanilla, Christine V Duffner, Lisa A Kovacs, Elizabeth J |
| description | Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2μg/ml)-stimulated splenocytes from sham treated animals produced 3557±853pg/ml of IFN-γ while splenocytes from burn injured animals released two-fold more cytokine (P |
| doi_str_mv | 10.1006/cyto.2000.0768 |
| format | Article |
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Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2μg/ml)-stimulated splenocytes from sham treated animals produced 3557±853pg/ml of IFN-γ while splenocytes from burn injured animals released two-fold more cytokine (P<0.05). To determine whether leukocyte production of IFN-γ was under the influence of macrophages, splenic macrophage supernatants generated from burned animals were incubated with splenic lymphocytes from sham and burn animals. The amount of IFN-γ released by lymphocytes from sham animals increased when cultured with macrophages from burned mice (P<0.05). This suggests that the increase in IFN-γ production by unfractionated splenocytes in burned mice relative to sham treated animals is macrophage-dependent. Macrophage supernatants from burned mice released twice as much IL-6 as supernatants from sham animals (P<0.05), and when IL-6 was blocked in vivo, the amount of IFN-γ production in burned mice decreased to sham levels (P<0.05). Thus, IL-6 mediates IFN-γ production following burn.</description><identifier>ISSN: 1043-4666</identifier><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1006/cyto.2000.0768</identifier><identifier>PMID: 11052818</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; burn injury/IFN-γ/IL-6/immunity/macrophage ; Cells, Cultured ; Concanavalin A - pharmacology ; Enzyme-Linked Immunosorbent Assay ; Female ; Flow Cytometry ; Interferon-gamma - biosynthesis ; Interleukin-4 - biosynthesis ; Interleukin-6 - metabolism ; Interleukin-6 - physiology ; Leukocytes - metabolism ; Lipopolysaccharides - pharmacology ; Macrophages - metabolism ; Mice ; Mice, Inbred BALB C ; Microscopy, Fluorescence ; Spleen - cytology ; Temperature</subject><ispartof>Cytokine (Philadelphia, Pa.), 2000-11, Vol.12 (11), p.1669-1675</ispartof><rights>2000 Academic Press</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-bcfb0025f0c19d3d98d8025e2c0ed4c04dbcbf651f5acd7abf19ea1f25caf5a3</citedby><cites>FETCH-LOGICAL-c340t-bcfb0025f0c19d3d98d8025e2c0ed4c04dbcbf651f5acd7abf19ea1f25caf5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/cyto.2000.0768$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11052818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durbin, Elizabeth A</creatorcontrib><creatorcontrib>Gregory, Meredith S</creatorcontrib><creatorcontrib>Messingham, Kelly A.N</creatorcontrib><creatorcontrib>Fontanilla, Christine V</creatorcontrib><creatorcontrib>Duffner, Lisa A</creatorcontrib><creatorcontrib>Kovacs, Elizabeth J</creatorcontrib><title>THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2μg/ml)-stimulated splenocytes from sham treated animals produced 3557±853pg/ml of IFN-γ while splenocytes from burn injured animals released two-fold more cytokine (P<0.05). To determine whether leukocyte production of IFN-γ was under the influence of macrophages, splenic macrophage supernatants generated from burned animals were incubated with splenic lymphocytes from sham and burn animals. The amount of IFN-γ released by lymphocytes from sham animals increased when cultured with macrophages from burned mice (P<0.05). This suggests that the increase in IFN-γ production by unfractionated splenocytes in burned mice relative to sham treated animals is macrophage-dependent. Macrophage supernatants from burned mice released twice as much IL-6 as supernatants from sham animals (P<0.05), and when IL-6 was blocked in vivo, the amount of IFN-γ production in burned mice decreased to sham levels (P<0.05). Thus, IL-6 mediates IFN-γ production following burn.</description><subject>Animals</subject><subject>burn injury/IFN-γ/IL-6/immunity/macrophage</subject><subject>Cells, Cultured</subject><subject>Concanavalin A - pharmacology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Interleukin-6 - metabolism</subject><subject>Interleukin-6 - physiology</subject><subject>Leukocytes - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microscopy, Fluorescence</subject><subject>Spleen - cytology</subject><subject>Temperature</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1Og0AUhSdGY2t169Kwcgfe4WeAZdNOLUqLIXThagLzk2DaUhlq0ufyPXwmh9DElauZc-93T-49CN1jcDAAeeKnrnFcAHAgJNEFGmOIiQ3gepf93_dsnxAyQjdafxgq9sLwGo0whsCNcDRGWbGkVp6l1MoWVrIuaJ7SzWuytohRQ2FB82xt_3xbb3k238yKJOsblhnMV9M0fTfiZZPTubVKZvQWXalyq-Xd-Z2gYkGL2dJOs-dkNk1t7vnQ2RVXlVkyUMBxLDwRRyIyUrocpPA5-KLilSIBVkHJRVhWCseyxMoNeGlK3gQ9DraHtvk8St2xXa253G7LvWyOmoWuR3yIwIDOAPK20bqVih3aele2J4aB9QmyPkHWJ8j6BM3Aw9n5WO2k-MPPkRkgGgBpzvuqZcs0r-WeS1G3kndMNPV_3r_B3Xpj</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Durbin, Elizabeth A</creator><creator>Gregory, Meredith S</creator><creator>Messingham, Kelly A.N</creator><creator>Fontanilla, Christine V</creator><creator>Duffner, Lisa A</creator><creator>Kovacs, Elizabeth J</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001101</creationdate><title>THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE</title><author>Durbin, Elizabeth A ; Gregory, Meredith S ; Messingham, Kelly A.N ; Fontanilla, Christine V ; Duffner, Lisa A ; Kovacs, Elizabeth J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-bcfb0025f0c19d3d98d8025e2c0ed4c04dbcbf651f5acd7abf19ea1f25caf5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>burn injury/IFN-γ/IL-6/immunity/macrophage</topic><topic>Cells, Cultured</topic><topic>Concanavalin A - pharmacology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Interleukin-6 - metabolism</topic><topic>Interleukin-6 - physiology</topic><topic>Leukocytes - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microscopy, Fluorescence</topic><topic>Spleen - cytology</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durbin, Elizabeth A</creatorcontrib><creatorcontrib>Gregory, Meredith S</creatorcontrib><creatorcontrib>Messingham, Kelly A.N</creatorcontrib><creatorcontrib>Fontanilla, Christine V</creatorcontrib><creatorcontrib>Duffner, Lisa A</creatorcontrib><creatorcontrib>Kovacs, Elizabeth J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durbin, Elizabeth A</au><au>Gregory, Meredith S</au><au>Messingham, Kelly A.N</au><au>Fontanilla, Christine V</au><au>Duffner, Lisa A</au><au>Kovacs, Elizabeth J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>12</volume><issue>11</issue><spage>1669</spage><epage>1675</epage><pages>1669-1675</pages><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2μg/ml)-stimulated splenocytes from sham treated animals produced 3557±853pg/ml of IFN-γ while splenocytes from burn injured animals released two-fold more cytokine (P<0.05). To determine whether leukocyte production of IFN-γ was under the influence of macrophages, splenic macrophage supernatants generated from burned animals were incubated with splenic lymphocytes from sham and burn animals. The amount of IFN-γ released by lymphocytes from sham animals increased when cultured with macrophages from burned mice (P<0.05). This suggests that the increase in IFN-γ production by unfractionated splenocytes in burned mice relative to sham treated animals is macrophage-dependent. Macrophage supernatants from burned mice released twice as much IL-6 as supernatants from sham animals (P<0.05), and when IL-6 was blocked in vivo, the amount of IFN-γ production in burned mice decreased to sham levels (P<0.05). Thus, IL-6 mediates IFN-γ production following burn.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11052818</pmid><doi>10.1006/cyto.2000.0768</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals burn injury/IFN-γ/IL-6/immunity/macrophage Cells, Cultured Concanavalin A - pharmacology Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Interferon-gamma - biosynthesis Interleukin-4 - biosynthesis Interleukin-6 - metabolism Interleukin-6 - physiology Leukocytes - metabolism Lipopolysaccharides - pharmacology Macrophages - metabolism Mice Mice, Inbred BALB C Microscopy, Fluorescence Spleen - cytology Temperature |
| title | THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE |
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