A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species
Abstract A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fu...
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| Veröffentlicht in: | Planta medica 2001-12, Vol.67 (9), p.858-862 |
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| container_title | Planta medica |
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| creator | Koulman, Albert Bos, Rein Medarde, Manuel Pras, Niesko Quax, Wim J. |
| description | Abstract
A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 °C to 320 °C at 15 °C min
-1
). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in ANTHRISCUS SYLVESTRIS (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species. |
| doi_str_mv | 10.1055/s-2001-18849 |
| format | Article |
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A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 °C to 320 °C at 15 °C min
-1
). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in ANTHRISCUS SYLVESTRIS (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2001-18849</identifier><identifier>PMID: 11745025</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - analysis ; Agronomy. Soil science and plant productions ; Apiaceae ; Biological and medical sciences ; Chemical constitution ; Dioxoles - analysis ; Economic plant physiology ; Fundamental and applied biological sciences. Psychology ; Gas Chromatography-Mass Spectrometry ; General pharmacology ; Lignans - analysis ; Medical sciences ; Original Paper ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - chemistry ; Podophyllotoxin - analogs & derivatives ; Podophyllotoxin - analysis</subject><ispartof>Planta medica, 2001-12, Vol.67 (9), p.858-862</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-fe40deeafe45871737d0edba4a2b340f35a37dd71edbbce89a916534af3f64c43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2001-18849.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2001-18849$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,27901,27902,54534,54535</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14108757$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11745025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koulman, Albert</creatorcontrib><creatorcontrib>Bos, Rein</creatorcontrib><creatorcontrib>Medarde, Manuel</creatorcontrib><creatorcontrib>Pras, Niesko</creatorcontrib><creatorcontrib>Quax, Wim J.</creatorcontrib><title>A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 °C to 320 °C at 15 °C min
-1
). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in ANTHRISCUS SYLVESTRIS (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - analysis</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Apiaceae</subject><subject>Biological and medical sciences</subject><subject>Chemical constitution</subject><subject>Dioxoles - analysis</subject><subject>Economic plant physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>General pharmacology</subject><subject>Lignans - analysis</subject><subject>Medical sciences</subject><subject>Original Paper</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - chemistry</subject><subject>Podophyllotoxin - analogs & derivatives</subject><subject>Podophyllotoxin - analysis</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEFvEzEQRi1ERdPCjTPyBS5ly3jtze4eQ0RLpVStCJwtZ3fcuPJ6g8cLyr_HbSL1wmnkmafPnx5j7wVcCqiqL1SUAKIQTaPaV2wmlGwLKEvxms0AZFlAq-QpOyN6zJhqAd6wUyFqVUFZzdjfBb8ylLgJPV-7YeeRXy_57ZrfYtqOPbdj5Cv3EEzg93G0zrvwwF3gi5C20VE3Eae9_4OU8us55asbaZ-vmFxnvN_zH-hNwp7fexMSX--wc0hv2Yk1nvDdcZ6zX1fffi6_F6u765vlYlV0SrSpsKigRzR5Vk0taln3gP3GKFNupAIrK5NXfS3yctNh05pWzCupjJV2rjolz9mnQ-4ujr-nXFMPuTX63AXHiXRdyrlQ0Gbw8wHs4kgU0epddIOJey1AP4nWpJ9E62fRGf9wzJ02A_Yv8NFsBj4eAUPZg40mdI5eOCWgqas6cxcHLm0dDqgfxymGbOT_3_4DrQ-UNA</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Koulman, Albert</creator><creator>Bos, Rein</creator><creator>Medarde, Manuel</creator><creator>Pras, Niesko</creator><creator>Quax, Wim J.</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species</title><author>Koulman, Albert ; Bos, Rein ; Medarde, Manuel ; Pras, Niesko ; Quax, Wim J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-fe40deeafe45871737d0edba4a2b340f35a37dd71edbbce89a916534af3f64c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - analysis</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Apiaceae</topic><topic>Biological and medical sciences</topic><topic>Chemical constitution</topic><topic>Dioxoles - analysis</topic><topic>Economic plant physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>General pharmacology</topic><topic>Lignans - analysis</topic><topic>Medical sciences</topic><topic>Original Paper</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - chemistry</topic><topic>Podophyllotoxin - analogs & derivatives</topic><topic>Podophyllotoxin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koulman, Albert</creatorcontrib><creatorcontrib>Bos, Rein</creatorcontrib><creatorcontrib>Medarde, Manuel</creatorcontrib><creatorcontrib>Pras, Niesko</creatorcontrib><creatorcontrib>Quax, Wim J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koulman, Albert</au><au>Bos, Rein</au><au>Medarde, Manuel</au><au>Pras, Niesko</au><au>Quax, Wim J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2001-12-01</date><risdate>2001</risdate><volume>67</volume><issue>9</issue><spage>858</spage><epage>862</epage><pages>858-862</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 °C to 320 °C at 15 °C min
-1
). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in ANTHRISCUS SYLVESTRIS (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11745025</pmid><doi>10.1055/s-2001-18849</doi><tpages>5</tpages></addata></record> |
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| ispartof | Planta medica, 2001-12, Vol.67 (9), p.858-862 |
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| language | eng |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - analysis Agronomy. Soil science and plant productions Apiaceae Biological and medical sciences Chemical constitution Dioxoles - analysis Economic plant physiology Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry General pharmacology Lignans - analysis Medical sciences Original Paper Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - chemistry Podophyllotoxin - analogs & derivatives Podophyllotoxin - analysis |
| title | A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species |
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