Expression of a reporter gene after microinjection of mammalian artificial chromosomes into pronuclei of bovine zygotes

The introduction of mammalian artificial chromosomes (ACs) into zygotes represents an alternative, more predictive technology for the production of recombinant proteins in transgenic animals. The aim of these experiments was to examine the effects of artificial chromosome microinjection into bovine...

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Veröffentlicht in:	Molecular reproduction and development 2001-12, Vol.60 (4), p.433-438
Hauptverfasser:	Wang, Bin, Lazaris, A., Lindenbaum, M., Stewart, S., Co, D., Perez, C., Drayer, J., Karatzas, C.N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals artificial chromosome Biological and medical sciences Biotechnology Blastocyst - cytology Blastocyst - metabolism blastocysts Cattle Cell Nucleus - genetics Chromosomes, Artificial, Mammalian - genetics embryo development Female Fertilization Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation, Developmental Genes, Reporter - genetics Genetic engineering Genetic technics Green Fluorescent Proteins Lac Operon - genetics lacZ gene Luminescent Proteins - genetics Luminescent Proteins - metabolism Male mammalian artificial chromosomes Methods. Procedures. Technologies Microinjections Survival Rate transgenesis Transgenic animals Transgenic animals and transgenic plants Zygote - cytology Zygote - growth & development Zygote - metabolism
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