Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain

The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique...

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Veröffentlicht in:Brain research 2001-12, Vol.922 (2), p.209-215
Hauptverfasser: Nakamura, Yasuhiro, Yamamoto, Munehiko, Kumamaru, Eriko
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Yamamoto, Munehiko
Kumamaru, Eriko
description The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. This suggests that the variant VLDLR is important for the developing human brain and the full length VLDLR has modified functions in aged and Alzheimer brains.
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The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. 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Psychology</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Plaque, Amyloid - metabolism</subject><subject>Plaque, Amyloid - pathology</subject><subject>Receptors, LDL - genetics</subject><subject>Receptors, LDL - immunology</subject><subject>Receptors, LDL - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Senile plaque</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>VLDL receptor</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS1ERIbAJ4C8AcGiQ_nZ7VWEIl5SpCwS1pbbLk-MetqD3ROUv8fJjJJlVi5Lpx66h5B3DE4ZMP3lCgB0NxgjPgH7DIL10KkXZMWGnneaS3hJVo_IMXld65_2FcLAK3LMWC-FUWxF1ldpPaeYvJs90hzpcoP01pXk5oW6OdC4m6Zuwnm93NCYy6Y-Qlju6JT_0YBzTUur0zZvS14wzbSgx-2SC231WFya35Cj6KaKbw_vCfn9_dv1-c_u4vLHr_OvF52Xw7B0XEtkyjgZveOotYERuA4OvNYSXO-900HzMcJoRoUDBoe9hMFro72UQZyQj_u57ZK_O6yL3aTqcZrcjHlXbc-F6jmYZ0E2CKl7pRuo9qAvudaC0W5L2rhyZxnYexX2QYW9z9kCsw8qrGp97w8LduMGw1PXIfsGfDgArno3xdIUpPrECSWaS964sz2HLbfbhMVWn7DpCqnFvNiQ0zOn_AcFsqaW</recordid><startdate>20011220</startdate><enddate>20011220</enddate><creator>Nakamura, Yasuhiro</creator><creator>Yamamoto, Munehiko</creator><creator>Kumamaru, Eriko</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20011220</creationdate><title>Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain</title><author>Nakamura, Yasuhiro ; Yamamoto, Munehiko ; Kumamaru, Eriko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-264e159a4fca2e6690b026da0c6640a7cca6d62bf0b9b5e8edae7408c696c44d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>A full length form</topic><topic>A variant form</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alzheimer Disease - metabolism</topic><topic>Alzheimer Disease - pathology</topic><topic>Alzheimer Disease - physiopathology</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity - genetics</topic><topic>Apolipoproteins E - metabolism</topic><topic>Base Sequence - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Brain - cytology</topic><topic>Brain - embryology</topic><topic>Brain - metabolism</topic><topic>Developing human brain</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Epitopes - immunology</topic><topic>Female</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Plaque, Amyloid - metabolism</topic><topic>Plaque, Amyloid - pathology</topic><topic>Receptors, LDL - genetics</topic><topic>Receptors, LDL - immunology</topic><topic>Receptors, LDL - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Senile plaque</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>VLDL receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, Yasuhiro</creatorcontrib><creatorcontrib>Yamamoto, Munehiko</creatorcontrib><creatorcontrib>Kumamaru, Eriko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, Yasuhiro</au><au>Yamamoto, Munehiko</au><au>Kumamaru, Eriko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2001-12-20</date><risdate>2001</risdate><volume>922</volume><issue>2</issue><spage>209</spage><epage>215</epage><pages>209-215</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. This suggests that the variant VLDLR is important for the developing human brain and the full length VLDLR has modified functions in aged and Alzheimer brains.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>11743951</pmid><doi>10.1016/S0006-8993(01)03170-5</doi><tpages>7</tpages></addata></record>
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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects A full length form
A variant form
Adult
Aged
Aged, 80 and over
Alzheimer Disease - metabolism
Alzheimer Disease - pathology
Alzheimer Disease - physiopathology
Animals
Antibodies, Monoclonal
Antibody Specificity - genetics
Apolipoproteins E - metabolism
Base Sequence - genetics
Biological and medical sciences
Blotting, Northern
Brain - cytology
Brain - embryology
Brain - metabolism
Developing human brain
Development. Senescence. Regeneration. Transplantation
Epitopes - immunology
Female
Fetus
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental - genetics
Humans
Immunohistochemistry
Infant
Infant, Newborn
Mice
Mice, Inbred BALB C
Middle Aged
Molecular Sequence Data
Neuroglia - cytology
Neuroglia - metabolism
Neurons - cytology
Neurons - metabolism
Plaque, Amyloid - metabolism
Plaque, Amyloid - pathology
Receptors, LDL - genetics
Receptors, LDL - immunology
Receptors, LDL - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Senile plaque
Vertebrates: nervous system and sense organs
VLDL receptor
title Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain
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