Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain
The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique...
Gespeichert in:
Veröffentlicht in: | Brain research 2001-12, Vol.922 (2), p.209-215 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 215 |
---|---|
container_issue | 2 |
container_start_page | 209 |
container_title | Brain research |
container_volume | 922 |
creator | Nakamura, Yasuhiro Yamamoto, Munehiko Kumamaru, Eriko |
description | The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. This suggests that the variant VLDLR is important for the developing human brain and the full length VLDLR has modified functions in aged and Alzheimer brains. |
doi_str_mv | 10.1016/S0006-8993(01)03170-5 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72357209</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006899301031705</els_id><sourcerecordid>18346756</sourcerecordid><originalsourceid>FETCH-LOGICAL-c488t-264e159a4fca2e6690b026da0c6640a7cca6d62bf0b9b5e8edae7408c696c44d3</originalsourceid><addsrcrecordid>eNqFkctuFDEQRS1ERIbAJ4C8AcGiQ_nZ7VWEIl5SpCwS1pbbLk-MetqD3ROUv8fJjJJlVi5Lpx66h5B3DE4ZMP3lCgB0NxgjPgH7DIL10KkXZMWGnneaS3hJVo_IMXld65_2FcLAK3LMWC-FUWxF1ldpPaeYvJs90hzpcoP01pXk5oW6OdC4m6Zuwnm93NCYy6Y-Qlju6JT_0YBzTUur0zZvS14wzbSgx-2SC231WFya35Cj6KaKbw_vCfn9_dv1-c_u4vLHr_OvF52Xw7B0XEtkyjgZveOotYERuA4OvNYSXO-900HzMcJoRoUDBoe9hMFro72UQZyQj_u57ZK_O6yL3aTqcZrcjHlXbc-F6jmYZ0E2CKl7pRuo9qAvudaC0W5L2rhyZxnYexX2QYW9z9kCsw8qrGp97w8LduMGw1PXIfsGfDgArno3xdIUpPrECSWaS964sz2HLbfbhMVWn7DpCqnFvNiQ0zOn_AcFsqaW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18346756</pqid></control><display><type>article</type><title>Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Nakamura, Yasuhiro ; Yamamoto, Munehiko ; Kumamaru, Eriko</creator><creatorcontrib>Nakamura, Yasuhiro ; Yamamoto, Munehiko ; Kumamaru, Eriko</creatorcontrib><description>The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. This suggests that the variant VLDLR is important for the developing human brain and the full length VLDLR has modified functions in aged and Alzheimer brains.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(01)03170-5</identifier><identifier>PMID: 11743951</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>A full length form ; A variant form ; Adult ; Aged ; Aged, 80 and over ; Alzheimer Disease - metabolism ; Alzheimer Disease - pathology ; Alzheimer Disease - physiopathology ; Animals ; Antibodies, Monoclonal ; Antibody Specificity - genetics ; Apolipoproteins E - metabolism ; Base Sequence - genetics ; Biological and medical sciences ; Blotting, Northern ; Brain - cytology ; Brain - embryology ; Brain - metabolism ; Developing human brain ; Development. Senescence. Regeneration. Transplantation ; Epitopes - immunology ; Female ; Fetus ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - genetics ; Humans ; Immunohistochemistry ; Infant ; Infant, Newborn ; Mice ; Mice, Inbred BALB C ; Middle Aged ; Molecular Sequence Data ; Neuroglia - cytology ; Neuroglia - metabolism ; Neurons - cytology ; Neurons - metabolism ; Plaque, Amyloid - metabolism ; Plaque, Amyloid - pathology ; Receptors, LDL - genetics ; Receptors, LDL - immunology ; Receptors, LDL - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Senile plaque ; Vertebrates: nervous system and sense organs ; VLDL receptor</subject><ispartof>Brain research, 2001-12, Vol.922 (2), p.209-215</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-264e159a4fca2e6690b026da0c6640a7cca6d62bf0b9b5e8edae7408c696c44d3</citedby><cites>FETCH-LOGICAL-c488t-264e159a4fca2e6690b026da0c6640a7cca6d62bf0b9b5e8edae7408c696c44d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(01)03170-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13538722$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11743951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakamura, Yasuhiro</creatorcontrib><creatorcontrib>Yamamoto, Munehiko</creatorcontrib><creatorcontrib>Kumamaru, Eriko</creatorcontrib><title>Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. This suggests that the variant VLDLR is important for the developing human brain and the full length VLDLR has modified functions in aged and Alzheimer brains.</description><subject>A full length form</subject><subject>A variant form</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alzheimer Disease - metabolism</subject><subject>Alzheimer Disease - pathology</subject><subject>Alzheimer Disease - physiopathology</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity - genetics</subject><subject>Apolipoproteins E - metabolism</subject><subject>Base Sequence - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Brain - cytology</subject><subject>Brain - embryology</subject><subject>Brain - metabolism</subject><subject>Developing human brain</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Epitopes - immunology</subject><subject>Female</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Plaque, Amyloid - metabolism</subject><subject>Plaque, Amyloid - pathology</subject><subject>Receptors, LDL - genetics</subject><subject>Receptors, LDL - immunology</subject><subject>Receptors, LDL - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Senile plaque</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>VLDL receptor</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS1ERIbAJ4C8AcGiQ_nZ7VWEIl5SpCwS1pbbLk-MetqD3ROUv8fJjJJlVi5Lpx66h5B3DE4ZMP3lCgB0NxgjPgH7DIL10KkXZMWGnneaS3hJVo_IMXld65_2FcLAK3LMWC-FUWxF1ldpPaeYvJs90hzpcoP01pXk5oW6OdC4m6Zuwnm93NCYy6Y-Qlju6JT_0YBzTUur0zZvS14wzbSgx-2SC231WFya35Cj6KaKbw_vCfn9_dv1-c_u4vLHr_OvF52Xw7B0XEtkyjgZveOotYERuA4OvNYSXO-900HzMcJoRoUDBoe9hMFro72UQZyQj_u57ZK_O6yL3aTqcZrcjHlXbc-F6jmYZ0E2CKl7pRuo9qAvudaC0W5L2rhyZxnYexX2QYW9z9kCsw8qrGp97w8LduMGw1PXIfsGfDgArno3xdIUpPrECSWaS964sz2HLbfbhMVWn7DpCqnFvNiQ0zOn_AcFsqaW</recordid><startdate>20011220</startdate><enddate>20011220</enddate><creator>Nakamura, Yasuhiro</creator><creator>Yamamoto, Munehiko</creator><creator>Kumamaru, Eriko</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20011220</creationdate><title>Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain</title><author>Nakamura, Yasuhiro ; Yamamoto, Munehiko ; Kumamaru, Eriko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-264e159a4fca2e6690b026da0c6640a7cca6d62bf0b9b5e8edae7408c696c44d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>A full length form</topic><topic>A variant form</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alzheimer Disease - metabolism</topic><topic>Alzheimer Disease - pathology</topic><topic>Alzheimer Disease - physiopathology</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity - genetics</topic><topic>Apolipoproteins E - metabolism</topic><topic>Base Sequence - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Brain - cytology</topic><topic>Brain - embryology</topic><topic>Brain - metabolism</topic><topic>Developing human brain</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Epitopes - immunology</topic><topic>Female</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Plaque, Amyloid - metabolism</topic><topic>Plaque, Amyloid - pathology</topic><topic>Receptors, LDL - genetics</topic><topic>Receptors, LDL - immunology</topic><topic>Receptors, LDL - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Senile plaque</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>VLDL receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, Yasuhiro</creatorcontrib><creatorcontrib>Yamamoto, Munehiko</creatorcontrib><creatorcontrib>Kumamaru, Eriko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, Yasuhiro</au><au>Yamamoto, Munehiko</au><au>Kumamaru, Eriko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2001-12-20</date><risdate>2001</risdate><volume>922</volume><issue>2</issue><spage>209</spage><epage>215</epage><pages>209-215</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The very low density lipoprotein receptor (VLDLR) is a newly described receptor which binds to apolipoprotein E (apoE) specifically. The authors designed a synthetic peptide of 17 amino acids representing the N-terminus of the putative first ligand binding domain of human VLDLR, this being a unique domain for VLDLR. When the synthetic peptide was used as the antigen, two different monoclonal antibodies were obtained (anti-VLDLR1 and anti-VLDLR2). Expressional cloning revealed that anti-VLDLR1 recognized the variant form of VLDLR which lacks 84 bp of O-linked sugar domain and anti-VLDLR2 recognized the full length form of VLDLR. The variant VLDLR was expressed in neuroblasts as well as matrix cells and Cajal-Retzius cells in the early stages of the developing human brain; later its expression was sequentially found in glioblasts, astrocytes, oligodendrocytes and finally in myelin. The expression of a full length form of VLDLR was detected in senile plaques and some neurons and satellite glia in aged and Alzheimer brains. This suggests that the variant VLDLR is important for the developing human brain and the full length VLDLR has modified functions in aged and Alzheimer brains.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>11743951</pmid><doi>10.1016/S0006-8993(01)03170-5</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0006-8993 |
ispartof | Brain research, 2001-12, Vol.922 (2), p.209-215 |
issn | 0006-8993 1872-6240 |
language | eng |
recordid | cdi_proquest_miscellaneous_72357209 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | A full length form A variant form Adult Aged Aged, 80 and over Alzheimer Disease - metabolism Alzheimer Disease - pathology Alzheimer Disease - physiopathology Animals Antibodies, Monoclonal Antibody Specificity - genetics Apolipoproteins E - metabolism Base Sequence - genetics Biological and medical sciences Blotting, Northern Brain - cytology Brain - embryology Brain - metabolism Developing human brain Development. Senescence. Regeneration. Transplantation Epitopes - immunology Female Fetus Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - genetics Humans Immunohistochemistry Infant Infant, Newborn Mice Mice, Inbred BALB C Middle Aged Molecular Sequence Data Neuroglia - cytology Neuroglia - metabolism Neurons - cytology Neurons - metabolism Plaque, Amyloid - metabolism Plaque, Amyloid - pathology Receptors, LDL - genetics Receptors, LDL - immunology Receptors, LDL - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Senile plaque Vertebrates: nervous system and sense organs VLDL receptor |
title | Significance of the variant and full-length forms of the very low density lipoprotein receptor in brain |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T15%3A23%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Significance%20of%20the%20variant%20and%20full-length%20forms%20of%20the%20very%20low%20density%20lipoprotein%20receptor%20in%20brain&rft.jtitle=Brain%20research&rft.au=Nakamura,%20Yasuhiro&rft.date=2001-12-20&rft.volume=922&rft.issue=2&rft.spage=209&rft.epage=215&rft.pages=209-215&rft.issn=0006-8993&rft.eissn=1872-6240&rft.coden=BRREAP&rft_id=info:doi/10.1016/S0006-8993(01)03170-5&rft_dat=%3Cproquest_cross%3E18346756%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18346756&rft_id=info:pmid/11743951&rft_els_id=S0006899301031705&rfr_iscdi=true |