Casein Secretion and Cytological Differentiation in Mammary Tissue from Bulls of High or Low Genetic Merit
We evaluated tissue content and secretion of milk proteins by mammary parenchymal explants prepared from tissue of mature Holstein bulls as a possible index for genetic merit. Explants from eight selection-line and eight control-line bulls were cultured for 48 or 96h in medium with 5% fetal bovine s...
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description | We evaluated tissue content and secretion of milk proteins by mammary parenchymal explants prepared from tissue of mature Holstein bulls as a possible index for genetic merit. Explants from eight selection-line and eight control-line bulls were cultured for 48 or 96h in medium with 5% fetal bovine serum with or without the addition of lactogenic hormones. Four selection-line and four control-line bulls were also treated for 7 d with estrogen and progesterone or placebo before tissue was removed on d 15 of the experiment. Overall, tissue content and secretion of casein were increased approximately twofold in selection-line bulls. Differences between genetic lines were evident only with the addition of lactogenic hormones. However, treatment of bulls with steroids was not necessary for casein secretion or detection of differences between genetic lines. Averaged for both lines, 96-h media concentrations of casein were 89-fold greater for explants cultured with added lactogenic hormones. Epithelial cells were classified as non-secretory, droplet-containing, or degenerated. The appearance of droplet-containing cells was markedly increased in cultures supplemented with lactogenic hormones, and the cells were most often located within epithelial folds or pockets. Only occasionally did we observe areas of alveolar-like tissue. The data demonstrate that it is possible to induce the secretion of casein in mammary explants prepared from sexually mature bulls, that lactogenic hormones markedly stimulate secretion, and that differences in genetic merit may be expressed. |
doi_str_mv | 10.3168/jds.S0022-0302(00)75110-1 |
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Explants from eight selection-line and eight control-line bulls were cultured for 48 or 96h in medium with 5% fetal bovine serum with or without the addition of lactogenic hormones. Four selection-line and four control-line bulls were also treated for 7 d with estrogen and progesterone or placebo before tissue was removed on d 15 of the experiment. Overall, tissue content and secretion of casein were increased approximately twofold in selection-line bulls. Differences between genetic lines were evident only with the addition of lactogenic hormones. However, treatment of bulls with steroids was not necessary for casein secretion or detection of differences between genetic lines. Averaged for both lines, 96-h media concentrations of casein were 89-fold greater for explants cultured with added lactogenic hormones. Epithelial cells were classified as non-secretory, droplet-containing, or degenerated. The appearance of droplet-containing cells was markedly increased in cultures supplemented with lactogenic hormones, and the cells were most often located within epithelial folds or pockets. Only occasionally did we observe areas of alveolar-like tissue. The data demonstrate that it is possible to induce the secretion of casein in mammary explants prepared from sexually mature bulls, that lactogenic hormones markedly stimulate secretion, and that differences in genetic merit may be expressed.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.S0022-0302(00)75110-1</identifier><identifier>PMID: 11049066</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Elsevier Inc</publisher><subject>Amino Acids - metabolism ; Animal productions ; Animals ; Biological and medical sciences ; Breeding ; Caseins - secretion ; Cattle - genetics ; Cattle - metabolism ; Culture Techniques - veterinary ; DNA - metabolism ; Epithelial Cells - cytology ; Estradiol - pharmacology ; Fundamental and applied biological sciences. Psychology ; genetic selection ; Isotope Labeling - veterinary ; Lactalbumin - secretion ; Male ; mammary culture ; Mammary Glands, Animal - cytology ; Mammary Glands, Animal - drug effects ; Mammary Glands, Animal - secretion ; Milk Proteins - secretion ; Mother. Fetoplacental unit. Mammary gland. Milk ; Pregnancy. Parturition. 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Explants from eight selection-line and eight control-line bulls were cultured for 48 or 96h in medium with 5% fetal bovine serum with or without the addition of lactogenic hormones. Four selection-line and four control-line bulls were also treated for 7 d with estrogen and progesterone or placebo before tissue was removed on d 15 of the experiment. Overall, tissue content and secretion of casein were increased approximately twofold in selection-line bulls. Differences between genetic lines were evident only with the addition of lactogenic hormones. However, treatment of bulls with steroids was not necessary for casein secretion or detection of differences between genetic lines. Averaged for both lines, 96-h media concentrations of casein were 89-fold greater for explants cultured with added lactogenic hormones. Epithelial cells were classified as non-secretory, droplet-containing, or degenerated. The appearance of droplet-containing cells was markedly increased in cultures supplemented with lactogenic hormones, and the cells were most often located within epithelial folds or pockets. Only occasionally did we observe areas of alveolar-like tissue. The data demonstrate that it is possible to induce the secretion of casein in mammary explants prepared from sexually mature bulls, that lactogenic hormones markedly stimulate secretion, and that differences in genetic merit may be expressed.</description><subject>Amino Acids - metabolism</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Breeding</subject><subject>Caseins - secretion</subject><subject>Cattle - genetics</subject><subject>Cattle - metabolism</subject><subject>Culture Techniques - veterinary</subject><subject>DNA - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Estradiol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic selection</subject><subject>Isotope Labeling - veterinary</subject><subject>Lactalbumin - secretion</subject><subject>Male</subject><subject>mammary culture</subject><subject>Mammary Glands, Animal - cytology</subject><subject>Mammary Glands, Animal - drug effects</subject><subject>Mammary Glands, Animal - secretion</subject><subject>Milk Proteins - secretion</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>progeny testing</subject><subject>Progesterone - pharmacology</subject><subject>Sexual Maturation</subject><subject>Terrestrial animal productions</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><subject>Vertebrates</subject><subject>Vertebrates: reproduction</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNqNkUtv1DAUhS0EotPCX0BGSDwWKX7GzhICtEhTsWhZWx7nuvUoiYudUPXf15kZFcSKlWX5O_cen4PQa0pOOa31x22XTy8JYawinLD3hHxQklJS0SdoRSWTFaeNfopWj8gROs55W66UEfkcHRVYNKSuV2jb2gxhxJfgEkwhjtiOHW7vp9jH6-Bsj78E7yHBOAW7ey_whR0Gm-7xVch5BuxTHPDnue8zjh6fh-sbHBNexzt8BmMZ6vAFpDC9QM-87TO8PJwn6Oe3r1ftebX-cfa9_bSunKRsqrqGuE5LcMppR6jnym8Y114TRTfCb7jQXNWCb8A5kKLxnaytELWoPRWMW36C3u7n3qb4a4Y8mSFkB31vR4hzNopxKaUSBXzzD7iNcxqLN0O1UkTVTKhCNXvKpZhzAm9uU1i-bygxSx2m1GF2dZgla0OI2dVhaNG-OmyYNwN0f5SH_P-yYHMJ2yc7upAfOU0EF4vRd3vqpmR7FxKYPNi-L0PpslzzxQpj9bKw3ZNQAv4dIJnsAowOuqJyk-li-A_bD5jNtbw</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Filep, R.</creator><creator>Akers, R.M.</creator><general>Elsevier Inc</general><general>Am Dairy Sci Assoc</general><general>American Dairy Science Association</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7WH</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Casein Secretion and Cytological Differentiation in Mammary Tissue from Bulls of High or Low Genetic Merit</title><author>Filep, R. ; Akers, R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-d90cd85ec7c8c01f37fb238f8071b4fb34837643becce549fd56a44646f1423a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acids - metabolism</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Breeding</topic><topic>Caseins - secretion</topic><topic>Cattle - genetics</topic><topic>Cattle - metabolism</topic><topic>Culture Techniques - veterinary</topic><topic>DNA - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Estradiol - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic selection</topic><topic>Isotope Labeling - veterinary</topic><topic>Lactalbumin - secretion</topic><topic>Male</topic><topic>mammary culture</topic><topic>Mammary Glands, Animal - cytology</topic><topic>Mammary Glands, Animal - drug effects</topic><topic>Mammary Glands, Animal - secretion</topic><topic>Milk Proteins - secretion</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>progeny testing</topic><topic>Progesterone - pharmacology</topic><topic>Sexual Maturation</topic><topic>Terrestrial animal productions</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><topic>Vertebrates</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filep, R.</creatorcontrib><creatorcontrib>Akers, R.M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 50</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filep, R.</au><au>Akers, R.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Casein Secretion and Cytological Differentiation in Mammary Tissue from Bulls of High or Low Genetic Merit</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>83</volume><issue>10</issue><spage>2261</spage><epage>2268</epage><pages>2261-2268</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>We evaluated tissue content and secretion of milk proteins by mammary parenchymal explants prepared from tissue of mature Holstein bulls as a possible index for genetic merit. Explants from eight selection-line and eight control-line bulls were cultured for 48 or 96h in medium with 5% fetal bovine serum with or without the addition of lactogenic hormones. Four selection-line and four control-line bulls were also treated for 7 d with estrogen and progesterone or placebo before tissue was removed on d 15 of the experiment. Overall, tissue content and secretion of casein were increased approximately twofold in selection-line bulls. Differences between genetic lines were evident only with the addition of lactogenic hormones. However, treatment of bulls with steroids was not necessary for casein secretion or detection of differences between genetic lines. Averaged for both lines, 96-h media concentrations of casein were 89-fold greater for explants cultured with added lactogenic hormones. Epithelial cells were classified as non-secretory, droplet-containing, or degenerated. The appearance of droplet-containing cells was markedly increased in cultures supplemented with lactogenic hormones, and the cells were most often located within epithelial folds or pockets. Only occasionally did we observe areas of alveolar-like tissue. The data demonstrate that it is possible to induce the secretion of casein in mammary explants prepared from sexually mature bulls, that lactogenic hormones markedly stimulate secretion, and that differences in genetic merit may be expressed.</abstract><cop>Savoy, IL</cop><pub>Elsevier Inc</pub><pmid>11049066</pmid><doi>10.3168/jds.S0022-0302(00)75110-1</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acids - metabolism Animal productions Animals Biological and medical sciences Breeding Caseins - secretion Cattle - genetics Cattle - metabolism Culture Techniques - veterinary DNA - metabolism Epithelial Cells - cytology Estradiol - pharmacology Fundamental and applied biological sciences. Psychology genetic selection Isotope Labeling - veterinary Lactalbumin - secretion Male mammary culture Mammary Glands, Animal - cytology Mammary Glands, Animal - drug effects Mammary Glands, Animal - secretion Milk Proteins - secretion Mother. Fetoplacental unit. Mammary gland. Milk Pregnancy. Parturition. Lactation progeny testing Progesterone - pharmacology Sexual Maturation Terrestrial animal productions Thymidine - metabolism Tritium Vertebrates Vertebrates: reproduction |
title | Casein Secretion and Cytological Differentiation in Mammary Tissue from Bulls of High or Low Genetic Merit |
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