Nerve-evoked secretion of immunoglobulin A in relation to other proteins by parotid glands in anaesthetized rat
Secretion of fluid and proteins by salivary cells is under the control of parasympathetic and sympathetic autonomic nerves. In a recent study we have shown that, in the rat submandibular gland, autonomic nerves can also increase the secretion of IgA, a product of plasma cells secreted into saliva as...
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| Veröffentlicht in: | Experimental physiology 2000-09, Vol.85 (5), p.511-518 |
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| creator | Proctor, G. B. Carpenter, G. H. Anderson, L. C. Garrett, J. R. |
| description | Secretion of fluid and proteins by salivary cells is under the control of
parasympathetic and sympathetic autonomic nerves. In a recent study we have shown
that, in the rat submandibular gland, autonomic nerves can also increase the
secretion of IgA, a product of plasma cells secreted into saliva as SIgA (IgA bound to
Secretory Component, the cleaved poly-immunoglobulin receptor). The present study
aimed to determine if parotid secretion of SIgA is increased by autonomic nerves and
to compare SIgA secretion with other parotid proteins stored and secreted by acinar
and ductal cells. Assay of IgA in saliva evoked by parasympathetic nerve stimulation
immediately following an extended rest period under anaesthesia indicated that it had
been secreted into intraductal saliva in the absence of stimulation during the rest
period. The mean rate of unstimulated IgA secretion (2.77 ± 0.28 µg min-1 g-1) and
the 2.5-fold increase in IgA secretion evoked by parasympathetic stimulation were
similar to results found previously in the rat submandibular gland. Sympathetic
nerve stimulation increased SIgA secretion 2.7-fold, much less than in the
submandibular gland. SDS-PAGE and Western blot analysis with anti-IgA and
anti-Secretory Component antibodies confirmed that SIgA was the predominant
form of IgA in saliva. Acinar-derived amylase and ductal-derived tissue kallikrein
were more profoundly increased by parasympathetic and particularly sympathetic
stimulation than SIgA. Overall, the results of the present study indicate that SIgA
forms a prominent component of unstimulated parotid salivary protein secretion and
that its secretion is similarly increased by stimulation of either autonomic nerve
supply. The secretion of other parotid salivary proteins that are synthesized and
stored by acinar or ductal cells is upregulated to a much greater extent by
parasympathetic and particularly sympathetic stimulation. Experimental Physiology
(2000) 85.5, 511-518. |
| doi_str_mv | 10.1111/j.1469-445X.2000.02060.x |
| format | Article |
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parasympathetic and sympathetic autonomic nerves. In a recent study we have shown
that, in the rat submandibular gland, autonomic nerves can also increase the
secretion of IgA, a product of plasma cells secreted into saliva as SIgA (IgA bound to
Secretory Component, the cleaved poly-immunoglobulin receptor). The present study
aimed to determine if parotid secretion of SIgA is increased by autonomic nerves and
to compare SIgA secretion with other parotid proteins stored and secreted by acinar
and ductal cells. Assay of IgA in saliva evoked by parasympathetic nerve stimulation
immediately following an extended rest period under anaesthesia indicated that it had
been secreted into intraductal saliva in the absence of stimulation during the rest
period. The mean rate of unstimulated IgA secretion (2.77 ± 0.28 µg min-1 g-1) and
the 2.5-fold increase in IgA secretion evoked by parasympathetic stimulation were
similar to results found previously in the rat submandibular gland. Sympathetic
nerve stimulation increased SIgA secretion 2.7-fold, much less than in the
submandibular gland. SDS-PAGE and Western blot analysis with anti-IgA and
anti-Secretory Component antibodies confirmed that SIgA was the predominant
form of IgA in saliva. Acinar-derived amylase and ductal-derived tissue kallikrein
were more profoundly increased by parasympathetic and particularly sympathetic
stimulation than SIgA. Overall, the results of the present study indicate that SIgA
forms a prominent component of unstimulated parotid salivary protein secretion and
that its secretion is similarly increased by stimulation of either autonomic nerve
supply. The secretion of other parotid salivary proteins that are synthesized and
stored by acinar or ductal cells is upregulated to a much greater extent by
parasympathetic and particularly sympathetic stimulation. Experimental Physiology
(2000) 85.5, 511-518.</description><identifier>ISSN: 0958-0670</identifier><identifier>EISSN: 1469-445X</identifier><identifier>DOI: 10.1111/j.1469-445X.2000.02060.x</identifier><identifier>PMID: 11038402</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Amylases - metabolism ; Amylases - secretion ; Anesthesia, Intravenous ; Anesthetics, Intravenous ; Animals ; Chloralose ; Electric Stimulation ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Immunoglobulin A - metabolism ; Male ; Parasympathetic Nervous System - physiology ; Parotid Gland - innervation ; Parotid Gland - metabolism ; Parotid Gland - secretion ; Rats ; Rats, Wistar ; Saliva - chemistry ; Salivary Proteins and Peptides - analysis ; Salivary Proteins and Peptides - metabolism ; Sympathetic Nervous System - physiology ; Tissue Kallikreins - metabolism ; Tissue Kallikreins - secretion</subject><ispartof>Experimental physiology, 2000-09, Vol.85 (5), p.511-518</ispartof><rights>The Physiological Society 2000</rights><rights>2000 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5300-3ccd1bcc8fc5d177624d05baf56f61605be9dbe0b85ae3d6f9b5e7d24e8449f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1469-445X.2000.02060.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1469-445X.2000.02060.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,1435,27931,27932,45581,45582,46416,46840</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11038402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Proctor, G. B.</creatorcontrib><creatorcontrib>Carpenter, G. H.</creatorcontrib><creatorcontrib>Anderson, L. C.</creatorcontrib><creatorcontrib>Garrett, J. R.</creatorcontrib><title>Nerve-evoked secretion of immunoglobulin A in relation to other proteins by parotid glands in anaesthetized rat</title><title>Experimental physiology</title><addtitle>Exp. physiol</addtitle><description>Secretion of fluid and proteins by salivary cells is under the control of
parasympathetic and sympathetic autonomic nerves. In a recent study we have shown
that, in the rat submandibular gland, autonomic nerves can also increase the
secretion of IgA, a product of plasma cells secreted into saliva as SIgA (IgA bound to
Secretory Component, the cleaved poly-immunoglobulin receptor). The present study
aimed to determine if parotid secretion of SIgA is increased by autonomic nerves and
to compare SIgA secretion with other parotid proteins stored and secreted by acinar
and ductal cells. Assay of IgA in saliva evoked by parasympathetic nerve stimulation
immediately following an extended rest period under anaesthesia indicated that it had
been secreted into intraductal saliva in the absence of stimulation during the rest
period. The mean rate of unstimulated IgA secretion (2.77 ± 0.28 µg min-1 g-1) and
the 2.5-fold increase in IgA secretion evoked by parasympathetic stimulation were
similar to results found previously in the rat submandibular gland. Sympathetic
nerve stimulation increased SIgA secretion 2.7-fold, much less than in the
submandibular gland. SDS-PAGE and Western blot analysis with anti-IgA and
anti-Secretory Component antibodies confirmed that SIgA was the predominant
form of IgA in saliva. Acinar-derived amylase and ductal-derived tissue kallikrein
were more profoundly increased by parasympathetic and particularly sympathetic
stimulation than SIgA. Overall, the results of the present study indicate that SIgA
forms a prominent component of unstimulated parotid salivary protein secretion and
that its secretion is similarly increased by stimulation of either autonomic nerve
supply. The secretion of other parotid salivary proteins that are synthesized and
stored by acinar or ductal cells is upregulated to a much greater extent by
parasympathetic and particularly sympathetic stimulation. Experimental Physiology
(2000) 85.5, 511-518.</description><subject>Amylases - metabolism</subject><subject>Amylases - secretion</subject><subject>Anesthesia, Intravenous</subject><subject>Anesthetics, Intravenous</subject><subject>Animals</subject><subject>Chloralose</subject><subject>Electric Stimulation</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Immunoglobulin A - metabolism</subject><subject>Male</subject><subject>Parasympathetic Nervous System - physiology</subject><subject>Parotid Gland - innervation</subject><subject>Parotid Gland - metabolism</subject><subject>Parotid Gland - secretion</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Saliva - chemistry</subject><subject>Salivary Proteins and Peptides - analysis</subject><subject>Salivary Proteins and Peptides - metabolism</subject><subject>Sympathetic Nervous System - physiology</subject><subject>Tissue Kallikreins - metabolism</subject><subject>Tissue Kallikreins - secretion</subject><issn>0958-0670</issn><issn>1469-445X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhiMEotvCKyCfEJcs48R2HCQOVVVopQo49MDNcuzJrpckXuykbXh6nO6K3gBbsseab8Yz82cZobCmab3frSkTdc4Y_74uAGANBQhYPzzLVn8cz7MV1FzmICo4yU5j3AHQEiR7mZ1QCqVkUKwy_wXDHeZ453-gJRFNwNH5gfiWuL6fBr_pfDN1biDnJB0BO_3oHz3x4xYD2Qc_ohsiaWay1-nhLNl0erBx4fWgMSZudL9S-qDHV9mLVncRXx_vs-z20-XtxVV-8_Xz9cX5TW54CZCXxljaGCNbwy2tKlEwC7zRLRetoCKZWNsGoZFcY2lFWzccK1swlIzVbXmWvT2kTeX9nFIJqnfRYJcKQz9FVRUlh1LIBL77K0hlTWVVgmAJlQfUBB9jwFbtg-t1mBUFtciidmqZvlqmrxZZ1KMs6iGFvjn-MjU92qfAow4J-HgA7l2H838nVpffrhbrqd-t22zvXUC1387R-eiNw3FWkqu0KU3gh2MTum-CsxtUOz-FIWnx7zZ-A3GBvaU</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>Proctor, G. B.</creator><creator>Carpenter, G. H.</creator><creator>Anderson, L. C.</creator><creator>Garrett, J. R.</creator><general>Cambridge University Press</general><general>The Physiological Society</general><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200009</creationdate><title>Nerve-evoked secretion of immunoglobulin A in relation to other proteins by parotid glands in anaesthetized rat</title><author>Proctor, G. B. ; Carpenter, G. H. ; Anderson, L. C. ; Garrett, J. 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B.</creatorcontrib><creatorcontrib>Carpenter, G. H.</creatorcontrib><creatorcontrib>Anderson, L. C.</creatorcontrib><creatorcontrib>Garrett, J. R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Proctor, G. B.</au><au>Carpenter, G. H.</au><au>Anderson, L. C.</au><au>Garrett, J. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nerve-evoked secretion of immunoglobulin A in relation to other proteins by parotid glands in anaesthetized rat</atitle><jtitle>Experimental physiology</jtitle><addtitle>Exp. physiol</addtitle><date>2000-09</date><risdate>2000</risdate><volume>85</volume><issue>5</issue><spage>511</spage><epage>518</epage><pages>511-518</pages><issn>0958-0670</issn><eissn>1469-445X</eissn><abstract>Secretion of fluid and proteins by salivary cells is under the control of
parasympathetic and sympathetic autonomic nerves. In a recent study we have shown
that, in the rat submandibular gland, autonomic nerves can also increase the
secretion of IgA, a product of plasma cells secreted into saliva as SIgA (IgA bound to
Secretory Component, the cleaved poly-immunoglobulin receptor). The present study
aimed to determine if parotid secretion of SIgA is increased by autonomic nerves and
to compare SIgA secretion with other parotid proteins stored and secreted by acinar
and ductal cells. Assay of IgA in saliva evoked by parasympathetic nerve stimulation
immediately following an extended rest period under anaesthesia indicated that it had
been secreted into intraductal saliva in the absence of stimulation during the rest
period. The mean rate of unstimulated IgA secretion (2.77 ± 0.28 µg min-1 g-1) and
the 2.5-fold increase in IgA secretion evoked by parasympathetic stimulation were
similar to results found previously in the rat submandibular gland. Sympathetic
nerve stimulation increased SIgA secretion 2.7-fold, much less than in the
submandibular gland. SDS-PAGE and Western blot analysis with anti-IgA and
anti-Secretory Component antibodies confirmed that SIgA was the predominant
form of IgA in saliva. Acinar-derived amylase and ductal-derived tissue kallikrein
were more profoundly increased by parasympathetic and particularly sympathetic
stimulation than SIgA. Overall, the results of the present study indicate that SIgA
forms a prominent component of unstimulated parotid salivary protein secretion and
that its secretion is similarly increased by stimulation of either autonomic nerve
supply. The secretion of other parotid salivary proteins that are synthesized and
stored by acinar or ductal cells is upregulated to a much greater extent by
parasympathetic and particularly sympathetic stimulation. Experimental Physiology
(2000) 85.5, 511-518.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>11038402</pmid><doi>10.1111/j.1469-445X.2000.02060.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Wiley Online Library (Open Access Collection) |
| subjects | Amylases - metabolism Amylases - secretion Anesthesia, Intravenous Anesthetics, Intravenous Animals Chloralose Electric Stimulation Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Immunoglobulin A - metabolism Male Parasympathetic Nervous System - physiology Parotid Gland - innervation Parotid Gland - metabolism Parotid Gland - secretion Rats Rats, Wistar Saliva - chemistry Salivary Proteins and Peptides - analysis Salivary Proteins and Peptides - metabolism Sympathetic Nervous System - physiology Tissue Kallikreins - metabolism Tissue Kallikreins - secretion |
| title | Nerve-evoked secretion of immunoglobulin A in relation to other proteins by parotid glands in anaesthetized rat |
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