Molecular characterization and evolution of the amylase multigene family of Drosophila ananassae
Drosophila ananassae is known to produce numerous alpha-amylase variants. We have cloned seven different Amy genes in an African strain homozygous for the AMY1,2,3,4 electrophoretic pattern. These genes are organized as two main clusters: the first one contains three intronless copies on the 2L chro...
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| Veröffentlicht in: | Journal of molecular evolution 2000-10, Vol.51 (4), p.391-403 |
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| description | Drosophila ananassae is known to produce numerous alpha-amylase variants. We have cloned seven different Amy genes in an African strain homozygous for the AMY1,2,3,4 electrophoretic pattern. These genes are organized as two main clusters: the first one contains three intronless copies on the 2L chromosome arm, two of which are tandemly arranged. The other cluster, on the 3L arm, contains two intron-bearing copies. The amylase variants AMY1 and AMY2 have been assigned to the intronless cluster, and AMY3 and AMY4 to the second one. The divergence of coding sequences between clusters is moderate (6.1% in amino acids), but the flanking regions are very different, which could explain their differential regulation. Within each cluster, coding and noncoding regions are conserved. Two very divergent genes were also cloned, both on chromosome 3L, but very distant from each other and from the other genes. One is the Amyrel homologous (41% divergent), the second one, Amyc1 (21.6% divergent) is unknown outside the D. ananassae subgroup. These two genes have unknown functions. |
| doi_str_mv | 10.1007/s002390010102 |
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We have cloned seven different Amy genes in an African strain homozygous for the AMY1,2,3,4 electrophoretic pattern. These genes are organized as two main clusters: the first one contains three intronless copies on the 2L chromosome arm, two of which are tandemly arranged. The other cluster, on the 3L arm, contains two intron-bearing copies. The amylase variants AMY1 and AMY2 have been assigned to the intronless cluster, and AMY3 and AMY4 to the second one. The divergence of coding sequences between clusters is moderate (6.1% in amino acids), but the flanking regions are very different, which could explain their differential regulation. Within each cluster, coding and noncoding regions are conserved. Two very divergent genes were also cloned, both on chromosome 3L, but very distant from each other and from the other genes. One is the Amyrel homologous (41% divergent), the second one, Amyc1 (21.6% divergent) is unknown outside the D. ananassae subgroup. These two genes have unknown functions.</description><identifier>ISSN: 0022-2844</identifier><identifier>EISSN: 1432-1432</identifier><identifier>DOI: 10.1007/s002390010102</identifier><identifier>PMID: 11040291</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Africa ; Amino Acid Sequence ; Amino acids ; AMY1 gene ; AMY2 gene ; AMY3 gene ; AMY4 gene ; Amylases - genetics ; Amyrel gene ; Animals ; Base Sequence ; chromosome 3L ; Drosophila - genetics ; Drosophila ananassae ; Enzymes ; Evolution, Molecular ; Evolutionary biology ; Gene Dosage ; Genetics, Population ; Insects ; Introns ; Molecular biology ; Molecular Sequence Data ; Multigene Family ; Population genetics ; Sequence Homology, Nucleic Acid</subject><ispartof>Journal of molecular evolution, 2000-10, Vol.51 (4), p.391-403</ispartof><rights>Springer-Verlag New York Inc. 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-18cc3f44347d5773f5758fa98b668cbe659cf70df84c1cf3ae05904c1191642a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11040291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Da Lage, J L</creatorcontrib><creatorcontrib>Maczkowiak, F</creatorcontrib><creatorcontrib>Cariou, M L</creatorcontrib><title>Molecular characterization and evolution of the amylase multigene family of Drosophila ananassae</title><title>Journal of molecular evolution</title><addtitle>J Mol Evol</addtitle><description>Drosophila ananassae is known to produce numerous alpha-amylase variants. We have cloned seven different Amy genes in an African strain homozygous for the AMY1,2,3,4 electrophoretic pattern. These genes are organized as two main clusters: the first one contains three intronless copies on the 2L chromosome arm, two of which are tandemly arranged. The other cluster, on the 3L arm, contains two intron-bearing copies. The amylase variants AMY1 and AMY2 have been assigned to the intronless cluster, and AMY3 and AMY4 to the second one. The divergence of coding sequences between clusters is moderate (6.1% in amino acids), but the flanking regions are very different, which could explain their differential regulation. Within each cluster, coding and noncoding regions are conserved. Two very divergent genes were also cloned, both on chromosome 3L, but very distant from each other and from the other genes. One is the Amyrel homologous (41% divergent), the second one, Amyc1 (21.6% divergent) is unknown outside the D. ananassae subgroup. These two genes have unknown functions.</description><subject>Africa</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>AMY1 gene</subject><subject>AMY2 gene</subject><subject>AMY3 gene</subject><subject>AMY4 gene</subject><subject>Amylases - genetics</subject><subject>Amyrel gene</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>chromosome 3L</subject><subject>Drosophila - genetics</subject><subject>Drosophila ananassae</subject><subject>Enzymes</subject><subject>Evolution, Molecular</subject><subject>Evolutionary biology</subject><subject>Gene Dosage</subject><subject>Genetics, Population</subject><subject>Insects</subject><subject>Introns</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Population genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0022-2844</issn><issn>1432-1432</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkctLAzEQxoMotlaPXmXx4G01r002R6lPULzoeU3Tid2S3dRkV6h_vVlbEL3IwDyY33wwfAgdE3xOMJYXEWPKFMYkBd1BY8IZzYe0i8ZpRXNacj5CBzEuEyQLxfbRiBDMMVVkjF4fvQPTOx0ys9BBmw5C_am72reZbucZfHjXf0_eZt0CMt2snY6QNb3r6jdoIbO6qd162F8FH_1qUTudblPEqOEQ7VntIhxt6wS93Fw_T-_yh6fb--nlQ24Yl11OSmOY5TwN80JKZgtZlFarciZEaWYgCmWsxHNbckOMZRpwoXDqiSKCU80m6Gyjuwr-vYfYVU0dDTinW_B9rCRlXEkh_gWJlFRQhhN4-gdc-j606YlKCVYQSfgA5RvIpN9jAFutQt3osK4IrgaDql8GJf5kK9rPGpj_0FtH2BdVH4qj</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Da Lage, J L</creator><creator>Maczkowiak, F</creator><creator>Cariou, M L</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Molecular characterization and evolution of the amylase multigene family of Drosophila ananassae</title><author>Da Lage, J L ; Maczkowiak, F ; Cariou, M L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-18cc3f44347d5773f5758fa98b668cbe659cf70df84c1cf3ae05904c1191642a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Africa</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>AMY1 gene</topic><topic>AMY2 gene</topic><topic>AMY3 gene</topic><topic>AMY4 gene</topic><topic>Amylases - genetics</topic><topic>Amyrel gene</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>chromosome 3L</topic><topic>Drosophila - genetics</topic><topic>Drosophila ananassae</topic><topic>Enzymes</topic><topic>Evolution, Molecular</topic><topic>Evolutionary biology</topic><topic>Gene Dosage</topic><topic>Genetics, Population</topic><topic>Insects</topic><topic>Introns</topic><topic>Molecular biology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Population genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Da Lage, J L</creatorcontrib><creatorcontrib>Maczkowiak, F</creatorcontrib><creatorcontrib>Cariou, M L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Da Lage, J L</au><au>Maczkowiak, F</au><au>Cariou, M L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization and evolution of the amylase multigene family of Drosophila ananassae</atitle><jtitle>Journal of molecular evolution</jtitle><addtitle>J Mol Evol</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>51</volume><issue>4</issue><spage>391</spage><epage>403</epage><pages>391-403</pages><issn>0022-2844</issn><eissn>1432-1432</eissn><abstract>Drosophila ananassae is known to produce numerous alpha-amylase variants. We have cloned seven different Amy genes in an African strain homozygous for the AMY1,2,3,4 electrophoretic pattern. These genes are organized as two main clusters: the first one contains three intronless copies on the 2L chromosome arm, two of which are tandemly arranged. The other cluster, on the 3L arm, contains two intron-bearing copies. The amylase variants AMY1 and AMY2 have been assigned to the intronless cluster, and AMY3 and AMY4 to the second one. The divergence of coding sequences between clusters is moderate (6.1% in amino acids), but the flanking regions are very different, which could explain their differential regulation. Within each cluster, coding and noncoding regions are conserved. Two very divergent genes were also cloned, both on chromosome 3L, but very distant from each other and from the other genes. One is the Amyrel homologous (41% divergent), the second one, Amyc1 (21.6% divergent) is unknown outside the D. ananassae subgroup. These two genes have unknown functions.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>11040291</pmid><doi>10.1007/s002390010102</doi><tpages>13</tpages></addata></record> |
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| subjects | Africa Amino Acid Sequence Amino acids AMY1 gene AMY2 gene AMY3 gene AMY4 gene Amylases - genetics Amyrel gene Animals Base Sequence chromosome 3L Drosophila - genetics Drosophila ananassae Enzymes Evolution, Molecular Evolutionary biology Gene Dosage Genetics, Population Insects Introns Molecular biology Molecular Sequence Data Multigene Family Population genetics Sequence Homology, Nucleic Acid |
| title | Molecular characterization and evolution of the amylase multigene family of Drosophila ananassae |
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