Imprinting as a rapid technique for assessing the morphology of the central nervous system by immunofluorescence
This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10–15 μm), i...
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| Veröffentlicht in: | Journal of neuroscience methods 2000-07, Vol.100 (1), p.85-91 |
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| creator | Thomson, Christine E Griffiths, Ian R |
| description | This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10–15 μm), imprinting does not section the cells, but can result in the detachment of whole cells onto a glass surface for subsequent staining. The imprinting technique is simple and rapid and does not require prior fixation or embedding of the tissue. It has been used to evaluate antigens expressed at the cell surface, in myelin and in the cytoskeleton in the studies of normal and myelin mutant mice. Using the imprinting/immunofluorescence technique one can now assay the genotype of mouse strains that differ in their expression of cell surface antigens within 2 h. |
| doi_str_mv | 10.1016/S0165-0270(00)00237-5 |
| format | Article |
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This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10–15 μm), imprinting does not section the cells, but can result in the detachment of whole cells onto a glass surface for subsequent staining. The imprinting technique is simple and rapid and does not require prior fixation or embedding of the tissue. It has been used to evaluate antigens expressed at the cell surface, in myelin and in the cytoskeleton in the studies of normal and myelin mutant mice. 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Methods ; Glass - standards ; Immunofluorescence ; Imprint ; Mice ; Microscopy, Phase-Contrast - methods ; Neuroglia - cytology ; Neuroglia - metabolism ; Neurons - cytology ; Neurons - metabolism ; Oligodendroglia ; Plp ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 2000-07, Vol.100 (1), p.85-91</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c337t-b2f28d972348da2c3de0bd384cacf3f714a6b0a48c8a4fbb8e942dc55e0443463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0165-0270(00)00237-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=829940$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11040370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomson, Christine E</creatorcontrib><creatorcontrib>Griffiths, Ian R</creatorcontrib><title>Imprinting as a rapid technique for assessing the morphology of the central nervous system by immunofluorescence</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10–15 μm), imprinting does not section the cells, but can result in the detachment of whole cells onto a glass surface for subsequent staining. The imprinting technique is simple and rapid and does not require prior fixation or embedding of the tissue. It has been used to evaluate antigens expressed at the cell surface, in myelin and in the cytoskeleton in the studies of normal and myelin mutant mice. Using the imprinting/immunofluorescence technique one can now assay the genotype of mouse strains that differ in their expression of cell surface antigens within 2 h.</description><subject>Animals</subject><subject>Antigens, Surface - analysis</subject><subject>Axons</subject><subject>Biological and medical sciences</subject><subject>Central Nervous System - cytology</subject><subject>Central Nervous System - metabolism</subject><subject>Fluorescent Antibody Technique - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Glass - standards</subject><subject>Immunofluorescence</subject><subject>Imprint</subject><subject>Mice</subject><subject>Microscopy, Phase-Contrast - methods</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Oligodendroglia</subject><subject>Plp</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFFr1TAUx4Mo7u7qR1ACgsyHztMkbdOnIUPdYODDFHwLaXKyG2mbmrSD--1N773Mx0FIIPmdc_75EfKuhMsSyvrzfd6qAlgDFwCfABhviuoF2ZSyYUXdyN8vyeYJOSPnKf0BANFC_ZqclSUI4A1syHQ7TNGPsx8fqE5U06gnb-mMZjf6vwtSF2J-SJjSisw7pEOI0y704WFPgzvcGBznqHs6YnwMS6Jpn2YcaLenfhiWMbh-CRFTxgy-Ia-c7hO-PZ1b8uvb15_XN8Xdj--311_uCsN5Mxcdc0zatmFcSKuZ4Rahs1wKo43jrimFrjvQQhqphes6ia1g1lQVghBc1HxLPh77TjHkf6RZDT4n6Hs9Ys6oDp3brG1LqiNoYkgpolNZyKDjXpWgVtXqoFqtHhWsK6tWVa57fxqwdAPa_1Untxn4cAJ0Mrp3UY_GpydOsrYVK3V1pDDLePQYVTJ-FWV9RDMrG_wzQf4BRrydAQ</recordid><startdate>20000731</startdate><enddate>20000731</enddate><creator>Thomson, Christine E</creator><creator>Griffiths, Ian R</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000731</creationdate><title>Imprinting as a rapid technique for assessing the morphology of the central nervous system by immunofluorescence</title><author>Thomson, Christine E ; Griffiths, Ian R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-b2f28d972348da2c3de0bd384cacf3f714a6b0a48c8a4fbb8e942dc55e0443463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antigens, Surface - analysis</topic><topic>Axons</topic><topic>Biological and medical sciences</topic><topic>Central Nervous System - cytology</topic><topic>Central Nervous System - metabolism</topic><topic>Fluorescent Antibody Technique - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Glass - standards</topic><topic>Immunofluorescence</topic><topic>Imprint</topic><topic>Mice</topic><topic>Microscopy, Phase-Contrast - methods</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Oligodendroglia</topic><topic>Plp</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomson, Christine E</creatorcontrib><creatorcontrib>Griffiths, Ian R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomson, Christine E</au><au>Griffiths, Ian R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imprinting as a rapid technique for assessing the morphology of the central nervous system by immunofluorescence</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2000-07-31</date><risdate>2000</risdate><volume>100</volume><issue>1</issue><spage>85</spage><epage>91</epage><pages>85-91</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. 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| subjects | Animals Antigens, Surface - analysis Axons Biological and medical sciences Central Nervous System - cytology Central Nervous System - metabolism Fluorescent Antibody Technique - methods Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Glass - standards Immunofluorescence Imprint Mice Microscopy, Phase-Contrast - methods Neuroglia - cytology Neuroglia - metabolism Neurons - cytology Neurons - metabolism Oligodendroglia Plp Vertebrates: nervous system and sense organs |
| title | Imprinting as a rapid technique for assessing the morphology of the central nervous system by immunofluorescence |
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