Determination of autoantibodies to annexin XI in systemic autoimmune diseases
Annexin XI, a calcyclin-associated protein, has been shown to be identical to a 56,000 Da antigen recognized by antibodies found in sera from patients suffering from systemic autoimmune diseases. In this work hexahistidine-tagged recombinant annexin XI (His6-rAnn XI) was used as antigen in ELISA exp...
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| Veröffentlicht in: | Lupus 2000-01, Vol.9 (7), p.515-520 |
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| creator | Jùrgensen, C S Levantino, G Houen, G Jacobsen, S Halberg, P Ullman, S Khamashta, M A Asmussen, K Oxholm, P Jùrgensen, MK van Venrooij, W J Wiik, A |
| description | Annexin XI, a calcyclin-associated protein, has been shown to be identical to a 56,000 Da antigen recognized by antibodies found in sera from patients suffering from systemic autoimmune diseases. In this work hexahistidine-tagged recombinant annexin XI (His6-rAnn XI) was used as antigen in ELISA experiments for determination of autoantibodies to annexin XI in sera of patients with systemic rheumatic autoimmune diseases. Immunoblotting with HeLa cell extract and with His6-rAnn XI as antigen was used for confirmation of positive ELISA results. We found eleven anti-annexin XI positive sera (3.9%) out of 282 sera from patients with systemic rheumatic diseases. The highest number of annexin XI positive sera were found in primary antiphospholipid syndrome (3=17), and in subacute lupus erythematosus (1=6), while lower frequencies of positive sera were found in patients with systemic sclerosis (5=137), rheumatoid arthritis (1=21), and systemic lupus erythematosus (1=58). Sera from healthy donors and patients with chronic infections were negative, except for one Salmonella typhimurium antibody positive serum. Autoantibodies to annexin XI were found to relate to thrombosis, but not to other clinical or laboratory features. A relation between antibodies to annexins and thrombosis has so far only been known for annexin V. |
| doi_str_mv | 10.1177/096120330000900707 |
| format | Article |
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In this work hexahistidine-tagged recombinant annexin XI (His6-rAnn XI) was used as antigen in ELISA experiments for determination of autoantibodies to annexin XI in sera of patients with systemic rheumatic autoimmune diseases. Immunoblotting with HeLa cell extract and with His6-rAnn XI as antigen was used for confirmation of positive ELISA results. We found eleven anti-annexin XI positive sera (3.9%) out of 282 sera from patients with systemic rheumatic diseases. The highest number of annexin XI positive sera were found in primary antiphospholipid syndrome (3=17), and in subacute lupus erythematosus (1=6), while lower frequencies of positive sera were found in patients with systemic sclerosis (5=137), rheumatoid arthritis (1=21), and systemic lupus erythematosus (1=58). Sera from healthy donors and patients with chronic infections were negative, except for one Salmonella typhimurium antibody positive serum. Autoantibodies to annexin XI were found to relate to thrombosis, but not to other clinical or laboratory features. A relation between antibodies to annexins and thrombosis has so far only been known for annexin V.</description><identifier>ISSN: 0961-2033</identifier><identifier>EISSN: 1477-0962</identifier><identifier>DOI: 10.1177/096120330000900707</identifier><identifier>PMID: 11035417</identifier><language>eng</language><publisher>Thousand Oaks, CA: SAGE Publications</publisher><subject>Adult ; Aged ; annexin V ; Annexins - immunology ; Antibodies ; Antigens ; Antiphospholipid Syndrome - blood ; Antiphospholipid Syndrome - immunology ; Arthritis, Rheumatoid - blood ; Arthritis, Rheumatoid - immunology ; Autoantibodies - blood ; Autoimmune diseases ; Autoimmune Diseases - blood ; Autoimmune Diseases - immunology ; Biochemistry ; Enzyme-Linked Immunosorbent Assay ; Female ; HeLa Cells ; Humans ; Localization ; Lupus ; Lupus Erythematosus, Systemic - blood ; Lupus Erythematosus, Systemic - immunology ; Male ; Middle Aged ; Proteins ; Recombinant Proteins - immunology ; Reproducibility of Results ; Rheumatoid arthritis ; Rheumatology ; Scleroderma ; Scleroderma, Systemic - blood ; Scleroderma, Systemic - immunology ; Thrombosis</subject><ispartof>Lupus, 2000-01, Vol.9 (7), p.515-520</ispartof><rights>Arnold 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-98676f1b977d6b7dca86d625753e148a1c6f9fcc1864c17f350e635263531333</citedby><cites>FETCH-LOGICAL-c463t-98676f1b977d6b7dca86d625753e148a1c6f9fcc1864c17f350e635263531333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/096120330000900707$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/096120330000900707$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11035417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jùrgensen, C S</creatorcontrib><creatorcontrib>Levantino, G</creatorcontrib><creatorcontrib>Houen, G</creatorcontrib><creatorcontrib>Jacobsen, S</creatorcontrib><creatorcontrib>Halberg, P</creatorcontrib><creatorcontrib>Ullman, S</creatorcontrib><creatorcontrib>Khamashta, M A</creatorcontrib><creatorcontrib>Asmussen, K</creatorcontrib><creatorcontrib>Oxholm, P</creatorcontrib><creatorcontrib>Jùrgensen, MK</creatorcontrib><creatorcontrib>van Venrooij, W J</creatorcontrib><creatorcontrib>Wiik, A</creatorcontrib><title>Determination of autoantibodies to annexin XI in systemic autoimmune diseases</title><title>Lupus</title><addtitle>Lupus</addtitle><description>Annexin XI, a calcyclin-associated protein, has been shown to be identical to a 56,000 Da antigen recognized by antibodies found in sera from patients suffering from systemic autoimmune diseases. In this work hexahistidine-tagged recombinant annexin XI (His6-rAnn XI) was used as antigen in ELISA experiments for determination of autoantibodies to annexin XI in sera of patients with systemic rheumatic autoimmune diseases. Immunoblotting with HeLa cell extract and with His6-rAnn XI as antigen was used for confirmation of positive ELISA results. We found eleven anti-annexin XI positive sera (3.9%) out of 282 sera from patients with systemic rheumatic diseases. The highest number of annexin XI positive sera were found in primary antiphospholipid syndrome (3=17), and in subacute lupus erythematosus (1=6), while lower frequencies of positive sera were found in patients with systemic sclerosis (5=137), rheumatoid arthritis (1=21), and systemic lupus erythematosus (1=58). Sera from healthy donors and patients with chronic infections were negative, except for one Salmonella typhimurium antibody positive serum. Autoantibodies to annexin XI were found to relate to thrombosis, but not to other clinical or laboratory features. A relation between antibodies to annexins and thrombosis has so far only been known for annexin V.</description><subject>Adult</subject><subject>Aged</subject><subject>annexin V</subject><subject>Annexins - immunology</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antiphospholipid Syndrome - blood</subject><subject>Antiphospholipid Syndrome - immunology</subject><subject>Arthritis, Rheumatoid - blood</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Autoantibodies - blood</subject><subject>Autoimmune diseases</subject><subject>Autoimmune Diseases - blood</subject><subject>Autoimmune Diseases - immunology</subject><subject>Biochemistry</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Localization</subject><subject>Lupus</subject><subject>Lupus Erythematosus, Systemic - blood</subject><subject>Lupus Erythematosus, Systemic - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Proteins</subject><subject>Recombinant Proteins - immunology</subject><subject>Reproducibility of Results</subject><subject>Rheumatoid arthritis</subject><subject>Rheumatology</subject><subject>Scleroderma</subject><subject>Scleroderma, Systemic - blood</subject><subject>Scleroderma, Systemic - immunology</subject><subject>Thrombosis</subject><issn>0961-2033</issn><issn>1477-0962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc9LwzAUx4Mobk7_AQ9SPHiry0vapD3K_DWYeNnBW0nTV8lYk9m04P57UzcYKGjgJYf3eZ9H-BJyCfQWQMopzQUwyjkNJ6dUUnlExpBIGYcOOybjAYgHYkTOvF8FjEMuTskIgPI0ATkmL_fYYdsYqzrjbOTqSPWdU7YzpasM-qhzkbIWP42N3uZRuP3Wd9gY_Q2apuktRpXxqDz6c3JSq7XHi_07IcvHh-XsOV68Ps1nd4tYJ4J3cZ4JKWoocykrUcpKq0xUgqUy5QhJpkCLOq-1hkwkGmTNU4qCpywUB875hNzstJvWffTou6IxXuN6rSy63heS8URCsP0HghR5lrEkgNc_wJXrWxv-UDDGBPCMDTa2g3TrvG-xLjataVS7LYAWQyLF70TC0NXe3JcNVoeRfQQBmO4Ar97xsPYP5ReD6JF-</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Jùrgensen, C S</creator><creator>Levantino, G</creator><creator>Houen, G</creator><creator>Jacobsen, S</creator><creator>Halberg, P</creator><creator>Ullman, S</creator><creator>Khamashta, M A</creator><creator>Asmussen, K</creator><creator>Oxholm, P</creator><creator>Jùrgensen, MK</creator><creator>van Venrooij, W J</creator><creator>Wiik, A</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20000101</creationdate><title>Determination of autoantibodies to annexin XI in systemic autoimmune diseases</title><author>Jùrgensen, C S ; Levantino, G ; Houen, G ; Jacobsen, S ; Halberg, P ; Ullman, S ; Khamashta, M A ; Asmussen, K ; Oxholm, P ; Jùrgensen, MK ; van Venrooij, W J ; Wiik, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-98676f1b977d6b7dca86d625753e148a1c6f9fcc1864c17f350e635263531333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Aged</topic><topic>annexin V</topic><topic>Annexins - immunology</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Antiphospholipid Syndrome - blood</topic><topic>Antiphospholipid Syndrome - immunology</topic><topic>Arthritis, Rheumatoid - blood</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Autoantibodies - blood</topic><topic>Autoimmune diseases</topic><topic>Autoimmune Diseases - blood</topic><topic>Autoimmune Diseases - immunology</topic><topic>Biochemistry</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Localization</topic><topic>Lupus</topic><topic>Lupus Erythematosus, Systemic - blood</topic><topic>Lupus Erythematosus, Systemic - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Proteins</topic><topic>Recombinant Proteins - immunology</topic><topic>Reproducibility of Results</topic><topic>Rheumatoid arthritis</topic><topic>Rheumatology</topic><topic>Scleroderma</topic><topic>Scleroderma, Systemic - blood</topic><topic>Scleroderma, Systemic - immunology</topic><topic>Thrombosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jùrgensen, C S</creatorcontrib><creatorcontrib>Levantino, G</creatorcontrib><creatorcontrib>Houen, G</creatorcontrib><creatorcontrib>Jacobsen, S</creatorcontrib><creatorcontrib>Halberg, P</creatorcontrib><creatorcontrib>Ullman, S</creatorcontrib><creatorcontrib>Khamashta, M A</creatorcontrib><creatorcontrib>Asmussen, K</creatorcontrib><creatorcontrib>Oxholm, P</creatorcontrib><creatorcontrib>Jùrgensen, MK</creatorcontrib><creatorcontrib>van Venrooij, W J</creatorcontrib><creatorcontrib>Wiik, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Lupus</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jùrgensen, C S</au><au>Levantino, G</au><au>Houen, G</au><au>Jacobsen, S</au><au>Halberg, P</au><au>Ullman, S</au><au>Khamashta, M A</au><au>Asmussen, K</au><au>Oxholm, P</au><au>Jùrgensen, MK</au><au>van Venrooij, W J</au><au>Wiik, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of autoantibodies to annexin XI in systemic autoimmune diseases</atitle><jtitle>Lupus</jtitle><addtitle>Lupus</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>9</volume><issue>7</issue><spage>515</spage><epage>520</epage><pages>515-520</pages><issn>0961-2033</issn><eissn>1477-0962</eissn><abstract>Annexin XI, a calcyclin-associated protein, has been shown to be identical to a 56,000 Da antigen recognized by antibodies found in sera from patients suffering from systemic autoimmune diseases. In this work hexahistidine-tagged recombinant annexin XI (His6-rAnn XI) was used as antigen in ELISA experiments for determination of autoantibodies to annexin XI in sera of patients with systemic rheumatic autoimmune diseases. Immunoblotting with HeLa cell extract and with His6-rAnn XI as antigen was used for confirmation of positive ELISA results. We found eleven anti-annexin XI positive sera (3.9%) out of 282 sera from patients with systemic rheumatic diseases. The highest number of annexin XI positive sera were found in primary antiphospholipid syndrome (3=17), and in subacute lupus erythematosus (1=6), while lower frequencies of positive sera were found in patients with systemic sclerosis (5=137), rheumatoid arthritis (1=21), and systemic lupus erythematosus (1=58). Sera from healthy donors and patients with chronic infections were negative, except for one Salmonella typhimurium antibody positive serum. Autoantibodies to annexin XI were found to relate to thrombosis, but not to other clinical or laboratory features. A relation between antibodies to annexins and thrombosis has so far only been known for annexin V.</abstract><cop>Thousand Oaks, CA</cop><pub>SAGE Publications</pub><pmid>11035417</pmid><doi>10.1177/096120330000900707</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Aged annexin V Annexins - immunology Antibodies Antigens Antiphospholipid Syndrome - blood Antiphospholipid Syndrome - immunology Arthritis, Rheumatoid - blood Arthritis, Rheumatoid - immunology Autoantibodies - blood Autoimmune diseases Autoimmune Diseases - blood Autoimmune Diseases - immunology Biochemistry Enzyme-Linked Immunosorbent Assay Female HeLa Cells Humans Localization Lupus Lupus Erythematosus, Systemic - blood Lupus Erythematosus, Systemic - immunology Male Middle Aged Proteins Recombinant Proteins - immunology Reproducibility of Results Rheumatoid arthritis Rheumatology Scleroderma Scleroderma, Systemic - blood Scleroderma, Systemic - immunology Thrombosis |
| title | Determination of autoantibodies to annexin XI in systemic autoimmune diseases |
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