Binding of external ligands onto an engineered virus capsid
The development of novel delivery systems for therapeutic substances includes targeting of the carriers to a specific site or tissue within the body of the recipient. This can be accomplished by appropriate receptor-binding domains and requires linking of these domains to the carrier. We have used r...
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| Veröffentlicht in: | Protein engineering 2001-10, Vol.14 (10), p.769-774 |
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| container_end_page | 774 |
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| container_issue | 10 |
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| container_title | Protein engineering |
| container_volume | 14 |
| creator | Schmidt, Uli Rudolph, Rainer Böhm, Gerald |
| description | The development of novel delivery systems for therapeutic substances includes targeting of the carriers to a specific site or tissue within the body of the recipient. This can be accomplished by appropriate receptor-binding domains and requires linking of these domains to the carrier. We have used recombinantly expressed polyomavirus-like particles as a model system and inserted the sequence of a WW domain into different surface loops of the viral capsid protein VP1. In one variant, the WW domain maintained its highly selective binding properties of proline-rich ligands and showed an increased affinity but also an accelerated association/dissociation equilibrium compared to the isolated WW domain, thus allowing a short-term coupling of external ligands onto the surface of the virus-like particles. |
| doi_str_mv | 10.1093/protein/14.10.769 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0269-2139 |
| ispartof | Protein engineering, 2001-10, Vol.14 (10), p.769-774 |
| issn | 0269-2139 1741-0126 1460-213X 1741-0134 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72346672 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Capsid - genetics Capsid - metabolism Capsid Proteins Carrier Proteins - genetics Carrier Proteins - metabolism cellular targeting Circular Dichroism Cloning, Molecular drug delivery Kinetics Ligands Mice Models, Molecular Polyomavirus - physiology polyomavirus VP1 Proline - metabolism Protein Binding protein design Protein Structure, Secondary Protein Structure, Tertiary Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism WW domain |
| title | Binding of external ligands onto an engineered virus capsid |
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