Identification of a Novel Fibroblast Growth Factor, FGF-23, Preferentially Expressed in the Ventrolateral Thalamic Nucleus of the Brain
We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus (∼24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect ce...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-10, Vol.277 (2), p.494-498 |
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| creator | Yamashita, Tetsuo Yoshioka, Masazumi Itoh, Nobuyuki |
| description | We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus (∼24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical (∼72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 (∼24% and ∼22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. Therefore, FGF-23 is expected a unique FGF that plays roles in the function of the ventrolateral thalamic nucleus. |
| doi_str_mv | 10.1006/bbrc.2000.3696 |
| format | Article |
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As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus (∼24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical (∼72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 (∼24% and ∼22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. 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As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus (∼24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical (∼72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 (∼24% and ∼22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. Therefore, FGF-23 is expected a unique FGF that plays roles in the function of the ventrolateral thalamic nucleus.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Baculoviridae - metabolism</subject><subject>Blotting, Western</subject><subject>Brain - metabolism</subject><subject>Cell Line</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 12</subject><subject>DNA, Complementary - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>FGF23 gene</subject><subject>Fibroblast Growth Factors - biosynthesis</subject><subject>Fibroblast Growth Factors - chemistry</subject><subject>Fibroblast Growth Factors - genetics</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Recombinant Proteins - metabolism</subject><subject>RNA, Complementary - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tissue Distribution</subject><subject>Ventral Thalamic Nuclei - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhyhH5xKlZ7NjrxEeomqVSVTgUxM1yxhOtkTdebKfQJ-C1SbQrcap6Gmnmm3-k-Qh5y9maM6Y-9H2Cdc0YWwul1TOy4kyzquZMPierua2qWvMfZ-RVzj8Z41wq_ZKccc5E3Ui9In-vHY7FDx5s8XGkcaCW3sZ7DLTzfYp9sLnQbYq_y452FkpMF7TbdlUtLujXhAOmZd-G8ECv_hwS5oyO-pGWHdLv8yjFYAsmG-jdzga790BvJwg45eXWQn1K1o-vyYvBhoxvTvWcfOuu7i4_VzdftteXH28qkKIpFWcbzRoJfeucs26juRUCaquV6AfXAMihVdgAAg6D062UPcq5XQPrLWgQ5-T9MfeQ4q8JczF7nwFDsCPGKZumFlKwVjwJ8ka1G6maGVwfQUgx5_kj5pD83qYHw5lZHJnFkVkcmcXRvPDulDz1e3T_8ZOUGWiPAM6PuPeYTAaPI6DzCaEYF_1j2f8AlaGiVw</recordid><startdate>20001022</startdate><enddate>20001022</enddate><creator>Yamashita, Tetsuo</creator><creator>Yoshioka, Masazumi</creator><creator>Itoh, Nobuyuki</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20001022</creationdate><title>Identification of a Novel Fibroblast Growth Factor, FGF-23, Preferentially Expressed in the Ventrolateral Thalamic Nucleus of the Brain</title><author>Yamashita, Tetsuo ; Yoshioka, Masazumi ; Itoh, Nobuyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-1059074cb8dddad591a33c2a963bfd7cc4f86e7ceceffd9844be47cc2c0bac9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Baculoviridae - metabolism</topic><topic>Blotting, Western</topic><topic>Brain - metabolism</topic><topic>Cell Line</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 12</topic><topic>DNA, Complementary - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>FGF23 gene</topic><topic>Fibroblast Growth Factors - biosynthesis</topic><topic>Fibroblast Growth Factors - chemistry</topic><topic>Fibroblast Growth Factors - genetics</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Recombinant Proteins - metabolism</topic><topic>RNA, Complementary - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tissue Distribution</topic><topic>Ventral Thalamic Nuclei - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamashita, Tetsuo</creatorcontrib><creatorcontrib>Yoshioka, Masazumi</creatorcontrib><creatorcontrib>Itoh, Nobuyuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamashita, Tetsuo</au><au>Yoshioka, Masazumi</au><au>Itoh, Nobuyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a Novel Fibroblast Growth Factor, FGF-23, Preferentially Expressed in the Ventrolateral Thalamic Nucleus of the Brain</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-10-22</date><risdate>2000</risdate><volume>277</volume><issue>2</issue><spage>494</spage><epage>498</epage><pages>494-498</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus (∼24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical (∼72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 (∼24% and ∼22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. 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| subjects | Amino Acid Sequence Animals Baculoviridae - metabolism Blotting, Western Brain - metabolism Cell Line Chromosome Mapping Chromosomes, Human, Pair 12 DNA, Complementary - metabolism Electrophoresis, Polyacrylamide Gel FGF23 gene Fibroblast Growth Factors - biosynthesis Fibroblast Growth Factors - chemistry Fibroblast Growth Factors - genetics Humans In Situ Hybridization Mice Molecular Sequence Data Phylogeny Recombinant Proteins - metabolism RNA, Complementary - metabolism RNA, Messenger - metabolism Sequence Homology, Amino Acid Tissue Distribution Ventral Thalamic Nuclei - metabolism |
| title | Identification of a Novel Fibroblast Growth Factor, FGF-23, Preferentially Expressed in the Ventrolateral Thalamic Nucleus of the Brain |
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