Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface
We have investigated the role that platelets may play in promoting adhesion of neutrophils to morphologically intact endothelium. Immortalized human microvascular endothelial cells (HMEC-1) were grown to confluence in a glass capillary (microslide) and incorporated in a flow-based assay that allowed...
Gespeichert in:
| Veröffentlicht in: | The Journal of laboratory and clinical medicine 2000-10, Vol.136 (4), p.303-313 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 313 |
|---|---|
| container_issue | 4 |
| container_start_page | 303 |
| container_title | The Journal of laboratory and clinical medicine |
| container_volume | 136 |
| creator | Kirton, Christopher M. Nash, Gerard B. |
| description | We have investigated the role that platelets may play in promoting adhesion of neutrophils to morphologically intact endothelium. Immortalized human microvascular endothelial cells (HMEC-1) were grown to confluence in a glass capillary (microslide) and incorporated in a flow-based assay that allowed video-microscopic quantitation of adhesive interactions of perfused, isolated neutrophils (wall shear rate 140 s–1). Platelets (with or without stimulation with thrombin) were first sedimented onto the HMEC-1 cells and formed discrete attachments covering |
| doi_str_mv | 10.1067/mlc.2000.109406 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72342146</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022214300355408</els_id><sourcerecordid>72342146</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-95d71ceb4e840174c25528604a13c5c20e52960037d5ef7f90c72c61305fb9e73</originalsourceid><addsrcrecordid>eNp1kU1vFSEYhYnR2Gt17c6wMO6m5WMYLsumqR9JEze6Jgy8eDEMXIGp6f_wB8vk3qRxIZsXyHNO4ByE3lJyRckkr5dorxgh20mNZHqGdlRwNkxi5M_RjhDGBkZHfoFe1fqzc4or-RJdUEq42gu6Q39ubAsPpoHDx9hHhFaxcQcokBpuGZuEQ2rGNgzJ5XaAGEzEFmLES045mkcoeA7JYR_z75B-4ARrK_l4CLE79XtIZo6Au3TZDFsxqfou2vYH-Me2rsUbC6_RC29ihTfneYm-f7z7dvt5uP_66cvtzf1gR75vgxJOUgvzCPuRUDlaJgTbT2Q0lFthGQHB1EQIl06Al14RK5mdKCfCzwokv0QfTr7Hkn-tUJteQt2-ZhLktWrJ-Njjmzp4fQJtybUW8PpYwmLKo6ZEb0XoXoTeitCnIrri3dl6nRdwT_w5-Q68PwOmWhN9T8WG-sQJ1pfqmDph0HN4CFB0tQGSBRcK2KZdDv99w1_G8qYY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72342146</pqid></control><display><type>article</type><title>Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Kirton, Christopher M. ; Nash, Gerard B.</creator><creatorcontrib>Kirton, Christopher M. ; Nash, Gerard B.</creatorcontrib><description>We have investigated the role that platelets may play in promoting adhesion of neutrophils to morphologically intact endothelium. Immortalized human microvascular endothelial cells (HMEC-1) were grown to confluence in a glass capillary (microslide) and incorporated in a flow-based assay that allowed video-microscopic quantitation of adhesive interactions of perfused, isolated neutrophils (wall shear rate 140 s–1). Platelets (with or without stimulation with thrombin) were first sedimented onto the HMEC-1 cells and formed discrete attachments covering <1% of the surface area. When neutrophils were perfused over the platelet-treated HMEC-1 cells, many short-lived adhesive interactions were seen (lasting ~0.3 seconds), whereas none were seen for monolayers without platelets. Few of these interactions converted to stationary adhesion, and only small numbers of neutrophils remained attached after a period of washout unless they were pre-stimulated with formyl peptide (fMLP; 10–7 mol/L). Then about 30% of adhesive interactions by activated neutrophils were seen to transform to a stationary adhesion, and numerous adherent cells remained after a period of washout. Studies with function-blocking antibodies showed that capture of the neutrophils was dependent on P-selectin exposed on platelets. Initial immobilization was mediated predominantly by the β2-integrin CD11b/CD18 expressed by neutrophils, but CD11a/CD18 also appeared to play a role in prolonged attachment. Visually, adhesion first occurred at sites occupied by platelets, but some activated neutrophils migrated onto the endothelial cells. These studies indicate that even small numbers of platelets that have adhered to morphologically intact endothelium have the potential to capture flowing neutrophils and facilitate their immobilization at the vessel wall and so promote inflammatory and thrombotic intercellular interactions. (J Lab Clin Med 2000; 136:303-13)</description><identifier>ISSN: 0022-2143</identifier><identifier>EISSN: 1532-6543</identifier><identifier>DOI: 10.1067/mlc.2000.109406</identifier><identifier>PMID: 11039851</identifier><identifier>CODEN: JLCMAK</identifier><language>eng</language><publisher>Saint Louis, MO: Mosby, Inc</publisher><subject>Adult ; Antibodies, Monoclonal ; Biological and medical sciences ; Blood Platelets - cytology ; CD18 Antigens - immunology ; CD18 Antigens - metabolism ; Cell Adhesion - drug effects ; Cell Adhesion - immunology ; Cell Communication - immunology ; Cell Line, Transformed ; Endothelium, Vascular - cytology ; Hematology ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Lymphocyte Function-Associated Antigen-1 - immunology ; Lymphocyte Function-Associated Antigen-1 - metabolism ; Macrophage-1 Antigen - immunology ; Macrophage-1 Antigen - metabolism ; Medical sciences ; N-Formylmethionine Leucyl-Phenylalanine - pharmacology ; Neutralization Tests ; Neutrophils - cytology ; Neutrophils - metabolism ; P-Selectin - immunology ; P-Selectin - metabolism ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Platelet Adhesiveness - physiology</subject><ispartof>The Journal of laboratory and clinical medicine, 2000-10, Vol.136 (4), p.303-313</ispartof><rights>2000 Mosby, Inc.</rights><rights>2000 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-95d71ceb4e840174c25528604a13c5c20e52960037d5ef7f90c72c61305fb9e73</citedby><cites>FETCH-LOGICAL-c438t-95d71ceb4e840174c25528604a13c5c20e52960037d5ef7f90c72c61305fb9e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1522229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11039851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kirton, Christopher M.</creatorcontrib><creatorcontrib>Nash, Gerard B.</creatorcontrib><title>Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface</title><title>The Journal of laboratory and clinical medicine</title><addtitle>J Lab Clin Med</addtitle><description>We have investigated the role that platelets may play in promoting adhesion of neutrophils to morphologically intact endothelium. Immortalized human microvascular endothelial cells (HMEC-1) were grown to confluence in a glass capillary (microslide) and incorporated in a flow-based assay that allowed video-microscopic quantitation of adhesive interactions of perfused, isolated neutrophils (wall shear rate 140 s–1). Platelets (with or without stimulation with thrombin) were first sedimented onto the HMEC-1 cells and formed discrete attachments covering <1% of the surface area. When neutrophils were perfused over the platelet-treated HMEC-1 cells, many short-lived adhesive interactions were seen (lasting ~0.3 seconds), whereas none were seen for monolayers without platelets. Few of these interactions converted to stationary adhesion, and only small numbers of neutrophils remained attached after a period of washout unless they were pre-stimulated with formyl peptide (fMLP; 10–7 mol/L). Then about 30% of adhesive interactions by activated neutrophils were seen to transform to a stationary adhesion, and numerous adherent cells remained after a period of washout. Studies with function-blocking antibodies showed that capture of the neutrophils was dependent on P-selectin exposed on platelets. Initial immobilization was mediated predominantly by the β2-integrin CD11b/CD18 expressed by neutrophils, but CD11a/CD18 also appeared to play a role in prolonged attachment. Visually, adhesion first occurred at sites occupied by platelets, but some activated neutrophils migrated onto the endothelial cells. These studies indicate that even small numbers of platelets that have adhered to morphologically intact endothelium have the potential to capture flowing neutrophils and facilitate their immobilization at the vessel wall and so promote inflammatory and thrombotic intercellular interactions. (J Lab Clin Med 2000; 136:303-13)</description><subject>Adult</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - cytology</subject><subject>CD18 Antigens - immunology</subject><subject>CD18 Antigens - metabolism</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - immunology</subject><subject>Cell Communication - immunology</subject><subject>Cell Line, Transformed</subject><subject>Endothelium, Vascular - cytology</subject><subject>Hematology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lymphocyte Function-Associated Antigen-1 - immunology</subject><subject>Lymphocyte Function-Associated Antigen-1 - metabolism</subject><subject>Macrophage-1 Antigen - immunology</subject><subject>Macrophage-1 Antigen - metabolism</subject><subject>Medical sciences</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Neutralization Tests</subject><subject>Neutrophils - cytology</subject><subject>Neutrophils - metabolism</subject><subject>P-Selectin - immunology</subject><subject>P-Selectin - metabolism</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Platelet Adhesiveness - physiology</subject><issn>0022-2143</issn><issn>1532-6543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1vFSEYhYnR2Gt17c6wMO6m5WMYLsumqR9JEze6Jgy8eDEMXIGp6f_wB8vk3qRxIZsXyHNO4ByE3lJyRckkr5dorxgh20mNZHqGdlRwNkxi5M_RjhDGBkZHfoFe1fqzc4or-RJdUEq42gu6Q39ubAsPpoHDx9hHhFaxcQcokBpuGZuEQ2rGNgzJ5XaAGEzEFmLES045mkcoeA7JYR_z75B-4ARrK_l4CLE79XtIZo6Au3TZDFsxqfou2vYH-Me2rsUbC6_RC29ihTfneYm-f7z7dvt5uP_66cvtzf1gR75vgxJOUgvzCPuRUDlaJgTbT2Q0lFthGQHB1EQIl06Al14RK5mdKCfCzwokv0QfTr7Hkn-tUJteQt2-ZhLktWrJ-Njjmzp4fQJtybUW8PpYwmLKo6ZEb0XoXoTeitCnIrri3dl6nRdwT_w5-Q68PwOmWhN9T8WG-sQJ1pfqmDph0HN4CFB0tQGSBRcK2KZdDv99w1_G8qYY</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Kirton, Christopher M.</creator><creator>Nash, Gerard B.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001001</creationdate><title>Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface</title><author>Kirton, Christopher M. ; Nash, Gerard B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-95d71ceb4e840174c25528604a13c5c20e52960037d5ef7f90c72c61305fb9e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - cytology</topic><topic>CD18 Antigens - immunology</topic><topic>CD18 Antigens - metabolism</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion - immunology</topic><topic>Cell Communication - immunology</topic><topic>Cell Line, Transformed</topic><topic>Endothelium, Vascular - cytology</topic><topic>Hematology</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lymphocyte Function-Associated Antigen-1 - immunology</topic><topic>Lymphocyte Function-Associated Antigen-1 - metabolism</topic><topic>Macrophage-1 Antigen - immunology</topic><topic>Macrophage-1 Antigen - metabolism</topic><topic>Medical sciences</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Neutralization Tests</topic><topic>Neutrophils - cytology</topic><topic>Neutrophils - metabolism</topic><topic>P-Selectin - immunology</topic><topic>P-Selectin - metabolism</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Platelet Adhesiveness - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Kirton, Christopher M.</creatorcontrib><creatorcontrib>Nash, Gerard B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of laboratory and clinical medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kirton, Christopher M.</au><au>Nash, Gerard B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface</atitle><jtitle>The Journal of laboratory and clinical medicine</jtitle><addtitle>J Lab Clin Med</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>136</volume><issue>4</issue><spage>303</spage><epage>313</epage><pages>303-313</pages><issn>0022-2143</issn><eissn>1532-6543</eissn><coden>JLCMAK</coden><abstract>We have investigated the role that platelets may play in promoting adhesion of neutrophils to morphologically intact endothelium. Immortalized human microvascular endothelial cells (HMEC-1) were grown to confluence in a glass capillary (microslide) and incorporated in a flow-based assay that allowed video-microscopic quantitation of adhesive interactions of perfused, isolated neutrophils (wall shear rate 140 s–1). Platelets (with or without stimulation with thrombin) were first sedimented onto the HMEC-1 cells and formed discrete attachments covering <1% of the surface area. When neutrophils were perfused over the platelet-treated HMEC-1 cells, many short-lived adhesive interactions were seen (lasting ~0.3 seconds), whereas none were seen for monolayers without platelets. Few of these interactions converted to stationary adhesion, and only small numbers of neutrophils remained attached after a period of washout unless they were pre-stimulated with formyl peptide (fMLP; 10–7 mol/L). Then about 30% of adhesive interactions by activated neutrophils were seen to transform to a stationary adhesion, and numerous adherent cells remained after a period of washout. Studies with function-blocking antibodies showed that capture of the neutrophils was dependent on P-selectin exposed on platelets. Initial immobilization was mediated predominantly by the β2-integrin CD11b/CD18 expressed by neutrophils, but CD11a/CD18 also appeared to play a role in prolonged attachment. Visually, adhesion first occurred at sites occupied by platelets, but some activated neutrophils migrated onto the endothelial cells. These studies indicate that even small numbers of platelets that have adhered to morphologically intact endothelium have the potential to capture flowing neutrophils and facilitate their immobilization at the vessel wall and so promote inflammatory and thrombotic intercellular interactions. (J Lab Clin Med 2000; 136:303-13)</abstract><cop>Saint Louis, MO</cop><pub>Mosby, Inc</pub><pmid>11039851</pmid><doi>10.1067/mlc.2000.109406</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2143 |
| ispartof | The Journal of laboratory and clinical medicine, 2000-10, Vol.136 (4), p.303-313 |
| issn | 0022-2143 1532-6543 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72342146 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Antibodies, Monoclonal Biological and medical sciences Blood Platelets - cytology CD18 Antigens - immunology CD18 Antigens - metabolism Cell Adhesion - drug effects Cell Adhesion - immunology Cell Communication - immunology Cell Line, Transformed Endothelium, Vascular - cytology Hematology Humans Investigative techniques, diagnostic techniques (general aspects) Lymphocyte Function-Associated Antigen-1 - immunology Lymphocyte Function-Associated Antigen-1 - metabolism Macrophage-1 Antigen - immunology Macrophage-1 Antigen - metabolism Medical sciences N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutralization Tests Neutrophils - cytology Neutrophils - metabolism P-Selectin - immunology P-Selectin - metabolism Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Platelet Adhesiveness - physiology |
| title | Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T08%3A56%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Activated%20platelets%20adherent%20to%20an%20intact%20endothelial%20cell%20monolayer%20bind%20flowing%20neutrophils%20and%20enable%20them%20to%20transfer%20to%20the%20endothelial%20surface&rft.jtitle=The%20Journal%20of%20laboratory%20and%20clinical%20medicine&rft.au=Kirton,%20Christopher%20M.&rft.date=2000-10-01&rft.volume=136&rft.issue=4&rft.spage=303&rft.epage=313&rft.pages=303-313&rft.issn=0022-2143&rft.eissn=1532-6543&rft.coden=JLCMAK&rft_id=info:doi/10.1067/mlc.2000.109406&rft_dat=%3Cproquest_cross%3E72342146%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72342146&rft_id=info:pmid/11039851&rft_els_id=S0022214300355408&rfr_iscdi=true |