Efficient phage display of polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein

We report that, contrary to common belief, polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein are functionally displayed on the phage surface. In a phagemid display system, carboxy-terminal fusion through optimized linker sequences resulted in display levels comparable t...

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Veröffentlicht in:	FEBS letters 2000-09, Vol.480 (2), p.231-234
Hauptverfasser:	Fuh, Germaine, Sidhu, Sachdev S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Artificial Gene Fusion Bacteriophage M13 - genetics Binding Sites Capsid Proteins Carboxy-terminus DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Endothelial Growth Factors - metabolism Lymphokines - metabolism M13 bacteriophage Minor coat protein Molecular Sequence Data P3, protein-3, the gene-3 minor coat protein of M13 phage P8, protein-8, the gene-8 major coat protein of M13 phage Peptide Library Peptides - genetics Peptides - metabolism Phage display Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Receptor Protein-Tyrosine Kinases - genetics Receptor Protein-Tyrosine Kinases - metabolism truncated P3, P3 residues 257–406 Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-1 Vascular Endothelial Growth Factors Viral Fusion Proteins - genetics Viral Fusion Proteins - metabolism
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