Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi
Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of dru...
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| Veröffentlicht in: | Southeast Asian journal of tropical medicine and public health 2000-03, Vol.31 (1), p.80-84 |
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| creator | PRACHAYASITTIKUL, Virapong LAWUNG, Ratana BULOW, Leif |
| description | Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred resistance to various beta-lactam antibiotics including penicillin G, carbenicillin, piperacillin, cefazolin, cefoperazone, ampicillin-sulbactam, and amoxicillin-clavulanate but not to cefoxitin. Co-resistance to streptomycin, chloramphenicol and tetracycline was not detected. Beta-lactamase gene was located on the major pCb fragment of EcoRI and AatII cutting. |
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The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred resistance to various beta-lactam antibiotics including penicillin G, carbenicillin, piperacillin, cefazolin, cefoperazone, ampicillin-sulbactam, and amoxicillin-clavulanate but not to cefoxitin. Co-resistance to streptomycin, chloramphenicol and tetracycline was not detected. Beta-lactamase gene was located on the major pCb fragment of EcoRI and AatII cutting.</description><identifier>ISSN: 0125-1562</identifier><identifier>PMID: 11023070</identifier><identifier>CODEN: SJTMAK</identifier><language>eng</language><publisher>Bangkok: Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network</publisher><subject>Bacterial diseases ; Bacterial diseases of the genital system ; beta-Lactam Resistance - genetics ; beta-Lactamases - genetics ; Biological and medical sciences ; Cloning, Molecular ; Drug Resistance, Microbial - genetics ; Drug Resistance, Multiple - genetics ; Escherichia coli - genetics ; Haemophilus ducreyi - drug effects ; Haemophilus ducreyi - genetics ; Human bacterial diseases ; Infectious diseases ; Medical sciences ; Plasmids ; Transformation, Bacterial</subject><ispartof>Southeast Asian journal of tropical medicine and public health, 2000-03, Vol.31 (1), p.80-84</ispartof><rights>2000 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1502068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11023070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PRACHAYASITTIKUL, Virapong</creatorcontrib><creatorcontrib>LAWUNG, Ratana</creatorcontrib><creatorcontrib>BULOW, Leif</creatorcontrib><title>Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi</title><title>Southeast Asian journal of tropical medicine and public health</title><addtitle>Southeast Asian J Trop Med Public Health</addtitle><description>Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred resistance to various beta-lactam antibiotics including penicillin G, carbenicillin, piperacillin, cefazolin, cefoperazone, ampicillin-sulbactam, and amoxicillin-clavulanate but not to cefoxitin. Co-resistance to streptomycin, chloramphenicol and tetracycline was not detected. Beta-lactamase gene was located on the major pCb fragment of EcoRI and AatII cutting.</description><subject>Bacterial diseases</subject><subject>Bacterial diseases of the genital system</subject><subject>beta-Lactam Resistance - genetics</subject><subject>beta-Lactamases - genetics</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Drug Resistance, Multiple - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Haemophilus ducreyi - drug effects</subject><subject>Haemophilus ducreyi - genetics</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Plasmids</subject><subject>Transformation, Bacterial</subject><issn>0125-1562</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0L1OwzAUBWAPIFoKr4A8ILZI_oudjqgqFKmCBebo2rkWRnYS4mQoT08kgjqd5dPR0bkga8ZFWfBSixW5zvmLMcX4troiK86ZkMywNXnd9yF3CWk_dD5EzBTahqbOhhh-wEakFkcoIrgREuTZRcgpNDS09ACYuv4zxCnTZnIDnsINufQQM94uuSEfT_v33aE4vj2_7B6PRc9LPhYgoGLCce2cVwwroUFoJZVWwhuQXFitUHpmnLUePJOlNn7bbK3h4IXUckMe_nrn2d8T5rFOITuMEVrsplwbIYUyUs3wboGTTdjU_RASDKf6_4EZ3C8AsoPoB2hdyGdXMsF0JX8BYVVjWg</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>PRACHAYASITTIKUL, Virapong</creator><creator>LAWUNG, Ratana</creator><creator>BULOW, Leif</creator><general>Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200003</creationdate><title>Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi</title><author>PRACHAYASITTIKUL, Virapong ; LAWUNG, Ratana ; BULOW, Leif</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p151t-a2a802c16ccf40e826a26434642f7a312b64e3f07cbbfaf03567f9d9b71af2363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Bacterial diseases</topic><topic>Bacterial diseases of the genital system</topic><topic>beta-Lactam Resistance - genetics</topic><topic>beta-Lactamases - genetics</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Drug Resistance, Multiple - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Haemophilus ducreyi - drug effects</topic><topic>Haemophilus ducreyi - genetics</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Plasmids</topic><topic>Transformation, Bacterial</topic><toplevel>online_resources</toplevel><creatorcontrib>PRACHAYASITTIKUL, Virapong</creatorcontrib><creatorcontrib>LAWUNG, Ratana</creatorcontrib><creatorcontrib>BULOW, Leif</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Southeast Asian journal of tropical medicine and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PRACHAYASITTIKUL, Virapong</au><au>LAWUNG, Ratana</au><au>BULOW, Leif</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi</atitle><jtitle>Southeast Asian journal of tropical medicine and public health</jtitle><addtitle>Southeast Asian J Trop Med Public Health</addtitle><date>2000-03</date><risdate>2000</risdate><volume>31</volume><issue>1</issue><spage>80</spage><epage>84</epage><pages>80-84</pages><issn>0125-1562</issn><coden>SJTMAK</coden><abstract>Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred resistance to various beta-lactam antibiotics including penicillin G, carbenicillin, piperacillin, cefazolin, cefoperazone, ampicillin-sulbactam, and amoxicillin-clavulanate but not to cefoxitin. Co-resistance to streptomycin, chloramphenicol and tetracycline was not detected. Beta-lactamase gene was located on the major pCb fragment of EcoRI and AatII cutting.</abstract><cop>Bangkok</cop><pub>Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network</pub><pmid>11023070</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Bacterial diseases Bacterial diseases of the genital system beta-Lactam Resistance - genetics beta-Lactamases - genetics Biological and medical sciences Cloning, Molecular Drug Resistance, Microbial - genetics Drug Resistance, Multiple - genetics Escherichia coli - genetics Haemophilus ducreyi - drug effects Haemophilus ducreyi - genetics Human bacterial diseases Infectious diseases Medical sciences Plasmids Transformation, Bacterial |
| title | Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi |
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