A gene expression screen in zebrafish embryogenesis
A screen for developmentally regulated genes was conducted in the zebrafish, a system offering substantial advantages for the study of the molecular genetics of vertebrate embryogenesis. Clones from a normalized cDNA library from early somitogenesis stages were picked randomly and tested by high-thr...
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| Veröffentlicht in: | Genome research 2001-12, Vol.11 (12), p.1979-1987 |
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| container_end_page | 1987 |
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| container_issue | 12 |
| container_start_page | 1979 |
| container_title | Genome research |
| container_volume | 11 |
| creator | Kudoh, T Tsang, M Hukriede, N A Chen, X Dedekian, M Clarke, C J Kiang, A Schultz, S Epstein, J A Toyama, R Dawid, I B |
| description | A screen for developmentally regulated genes was conducted in the zebrafish, a system offering substantial advantages for the study of the molecular genetics of vertebrate embryogenesis. Clones from a normalized cDNA library from early somitogenesis stages were picked randomly and tested by high-throughput in situ hybridization for restricted expression in at least one of four stages of development. Among 2765 clones that were screened, a total of 347 genes with patterns judged to be restricted were selected. These clones were subjected to partial sequence analysis, allowing recognition of functional motifs in 163 among them. In addition, a portion of the clones were mapped with the aid of the LN54 radiation hybrid panel. The usefulness of the in situ hybridization screening approach is illustrated by describing several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different regions of the developing brain. |
| doi_str_mv | 10.1101/gr.209601 |
| format | Article |
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The usefulness of the in situ hybridization screening approach is illustrated by describing several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different regions of the developing brain.</description><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Brain - physiology</subject><subject>Brain Chemistry - genetics</subject><subject>Chromosome Mapping - methods</subject><subject>Cloning, Molecular</subject><subject>Danio rerio</subject><subject>Databases, Genetic</subject><subject>DNA, Complementary - genetics</subject><subject>Egg Yolk - metabolism</subject><subject>Egg Yolk - physiology</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Embryonic Development</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Gene Library</subject><subject>Giant Cells - metabolism</subject><subject>Giant Cells - physiology</subject><subject>In Situ Hybridization - methods</subject><subject>Internet</subject><subject>Radiation Hybrid Mapping</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><issn>1088-9051</issn><issn>1054-9803</issn><issn>1549-5469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E9LAzEQBfAgiq3Vg19A9iR42JpJdrPJsRT_QcGLnkM2mdSV7m7NtGD99G5pwaOnmcOPx-Mxdg18CsDhfpmmghvF4YSNoSxMXhbKnA4_1zo3vIQRuyD65JzLQutzNgKoJBS6GjM5y5bYYYbf64RETd9l5BNilzVd9oN1crGhjwzbOu36vaSGLtlZdCvCq-OdsPfHh7f5c754fXqZzxa5L0BuchW9ls6DFy4IjV4JkMqrAEZHLQyHKMqhbCx9MC5IwZ3SoigwyqoOQQY5YbeH3HXqv7ZIG9s25HG1ch32W7KVkKCNhn8h6EEKrgZ4d4A-9UQJo12npnVpZ4Hb_ZR2mexhysHeHEO3dYvhTx63k7-CFW2B</recordid><startdate>20011201</startdate><enddate>20011201</enddate><creator>Kudoh, T</creator><creator>Tsang, M</creator><creator>Hukriede, N A</creator><creator>Chen, X</creator><creator>Dedekian, M</creator><creator>Clarke, C J</creator><creator>Kiang, A</creator><creator>Schultz, S</creator><creator>Epstein, J A</creator><creator>Toyama, R</creator><creator>Dawid, I B</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20011201</creationdate><title>A gene expression screen in zebrafish embryogenesis</title><author>Kudoh, T ; 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| language | eng |
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| subjects | Animals Brain - metabolism Brain - physiology Brain Chemistry - genetics Chromosome Mapping - methods Cloning, Molecular Danio rerio Databases, Genetic DNA, Complementary - genetics Egg Yolk - metabolism Egg Yolk - physiology Embryo, Nonmammalian - physiology Embryonic Development Gene Expression Profiling - methods Gene Expression Regulation, Developmental - genetics Gene Library Giant Cells - metabolism Giant Cells - physiology In Situ Hybridization - methods Internet Radiation Hybrid Mapping Zebrafish - embryology Zebrafish - genetics |
| title | A gene expression screen in zebrafish embryogenesis |
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